Archives of Microbiology

, Volume 147, Issue 1, pp 64–67 | Cite as

Characterization of the amine oxidase involved in the growth of Trichosporon cutaneum X4 on ethylamine as source of carbon, nitrogen and energy

  • P. J. Large
  • L. A. Sherlock
Original Papers


The amine oxidase from Trichosporon cutaneum X4 grown on ethylamine as carbon, nitrogen and energy source was purified to near homogeneity. The purified enzyme showed the highest resistance to heat of any amine oxidase hitherto characterized from a yeast (half-life at 62°C, 14 min). Measurement of kinetic parameters as a function of carbon chain length showed results typical of a benzylamine oxidase. Both non-denaturing- and sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed multiple bands, and dimethyl suberimidate cross-linking studies revealed that the enzyme consisted of multimers of two polypeptide chains of Mr respectively 19,000 and 26,000. The smallest structure to show activity probably contained two of each kind of subunit.

Key words

Trichosporon cutaneum X4 Ethylamine Benzylamine oxidase Heat resistance Subunit composition Dimethyl suberimidate cross-linking 



sodium dodecyl sulphate


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • P. J. Large
    • 1
  • L. A. Sherlock
    • 1
  1. 1.Department of BiochemistryUniversity of HullHullUK

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