Advertisement

Archives of Microbiology

, Volume 147, Issue 1, pp 21–24 | Cite as

Isolation of an extremely thermophilic chemoorganotrophic anaerobe similar to Dictyoglomus thermophilum from new zealand hot springs

  • B. K. Patel
  • H. W. Morgan
  • J. Wiegel
  • R. M. Daniel
Original Papers

Abstract

A strain similar to Dictyoglomus thermophilum, isolated from a New Zealand hot spring, is described. This strictly anaerobic, Gram-negative, non-motile and nonsporulating bacterium usually exists as long thin filaments of 5 to 25 μm by 0.35 to 0.45 μm. Rotund bodies are commonly observed. Thin sections of the cells revealed a two-layered cell wall. The optimum temperature and pH for growth was 70°C and 7.0 and 7.5 respectively. No growth was observed at 40°C and 85°C or at pH 4.5 to pH 9.0. The organism fermented glucose, maltose, mannose, xylose, lactose, cellobiose, galactose and sucrose and produced acetate as the major end-product with significant amounts of lactate, H2 and CO2 and only traces of ethanol. The doubling time on glucose was 10 h. The DNA base composition was 29.5% guanine plus cytosine as determined by the thermal denaturation method. Growth was inhibited by penicillin, tetracycline and chloramphenicol indicating that the organism was a eubacterium. These features are in common with the newly described species Dictyoglomus thermophilum to which the New Zealand isolate belongs.

Key words

Dictyoglomus Fervidothrix Description Rotund bodies Extremely thermophilic Obligate anaerobe Fermentation products Physiology Distribution 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ben-Bassat A, Zeikus JG (1981) Thermobacteroides acetoethylicus gen. nov. and spec. nov., a new chemoorganotrophic, anaerobic thermophilic bacterium. Arch Microbiol 128:265–370Google Scholar
  2. Brock TD, Freeze H (1969) Thermus aquaticus gen. nov. and spec. nov., a non-sporulating extreme thermophile. J Bacteriol 98:289–297Google Scholar
  3. Gutmann I, Wahlefeld AW (1974) l-(+)-Lactate determination with lactate dehydrogenase and NAD. In: Bergmeyer HU, Gawehu K (eds) Methods of enzymatic analysis, vol 3. Academic Press, New York, pp 1464–1468Google Scholar
  4. Johnson EA, Madia A, Demain AL (1981) Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophilie Clostridium thermocellum. Appl Environ Microbiol 41:1060–1062Google Scholar
  5. Lundie LL, Drake HL (1984) Development of a minimally defined medium for the acetogen Clostridium thermoaceticum. J Bacteriol 159:700–703Google Scholar
  6. Morgan HW, Patel BKC, Daniel RM (1985) Comparison of a Thermoanaerobium sp. from a New Zealand hot spring with Thermoanaerobium brockii. FEMS Microbiol Letts 30:121–124Google Scholar
  7. Patel BKC, Morgan HW, Daniel RM (1985a) Fervidobacterium nodosum gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol 141:63–69Google Scholar
  8. Patel BKC, Morgan HW, Daniel RM (1985b) Thermophilic anaerobic spirochetes in New Zealand hot springs. FEMS Microbiol Letts 26:101–106Google Scholar
  9. Patel BKC, Morgan HW, Daniel RM (1985c) A simple and efficient method for preparing and dispensing anaerobic media. Biotech Letts 7:277–278Google Scholar
  10. Patel BKC, Morgan HW, Daniel RM (1986a) Studies on some thermophilic glycolytic anaerobic bacteria from New Zealand hot springs. Syst Appl Microbiol 8:128–136Google Scholar
  11. Patel BKC, Morgan HW, Daniel RM (1985b) Unusual microorganisms observed in New Zealand hot springs. Microb Ecol 12:181–186Google Scholar
  12. Saiki T, Kobayashi Y, Kawagoe K, Beppu T (1985) Dictyoglomus thermophilum gen. nov., sp. nov., a chemo-organotrophic, anaerobic, thermophilic bacterium. Int J Syst Bacteriol 35:253–259Google Scholar
  13. Wiegel J, Ljungdahl LG (1981) Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new extreme thermophilic, anaerobic bacterium. Arch Microbiol 128:343–348Google Scholar
  14. Zeikus JG, Wolfe RS (1972) Methanobacterium thermoautotrophicum sp. n., an anaerobic, autotrophic, extreme thermophile. J Bacteriol 109:707–713Google Scholar
  15. Zeikus JF, Hegge PW, Anderson MA (1979) Thermoanaerobium brockii gen. nov. and spec. nov., a new chemoorganotrophic, caldoactive, anaerobic bacterium. Arch Microbiol 122:41–48Google Scholar
  16. Zillig W, Gierl A, Schreiber G, Wunderl S, Janekovic D, Stetter KO, Klenk HP (1983) The archaebacterium Thermofilum pendens represents a novel genus of the thermophilic, anaerobic sulfur respiring Thermoproteales. Syst Appl Microbiol 4:79–87Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • B. K. Patel
    • 1
  • H. W. Morgan
    • 1
  • J. Wiegel
    • 2
    • 3
  • R. M. Daniel
    • 1
  1. 1.Department of Biological SciencesUniversity of WaikatoHamiltonNew Zealand
  2. 2.Department of MicrobiologyUniversity of GeorgiaAthensUSA
  3. 3.Centre for Biological Resource RecoveryUniversity of GeorgiaAthensUSA

Personalised recommendations