, Volume 80, Issue 1, pp 45–48 | Cite as

Colloidal iron hydroxide staining of surface carbohydrates after glycerol treatment of uterine epithelial cells

  • C. R. Murphy
  • S. Bradbury


The structural relationships between intramembranous particles (IMPs) and surface carbohydrates has been studied in uterine epithelial cells with a colloidal iron hydroxide (CIH) technique. To aggregate IMPs, glycerol treatment of unfixed cells was used and this treatment also caused some patching of CIH deposits on the cell surface. We conclude that some of the CIH receptors may be the surface expresion of the IMPs.


Public Health Iron Epithelial Cell Glycerol Carbohydrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bearer EL, Friend DS (1980) Anionic lipid domains: correlation with functional topography in a mammalian cell membrane. Proc Natl Acad Sci USA 77:6601–6605Google Scholar
  2. Bradbury S, Billington WD, Kirby DRS, Williams EA (1970) Histochemical characterization of the surface mucoprotein of normal and abnormal human trophoblast. Histochem J 2:263–274Google Scholar
  3. Bullivant S (1977) Evaluation of membrane structure facts and artefacts during freeze-fracturing. J Microsc 111:101–116Google Scholar
  4. Everett JW (1948) Progresterone and estrogen in the experimental control of ovulation time and other features of the estrous cycle in the rat. Endocrinology 43:389–405Google Scholar
  5. Gasic GJ, Berwick L, Sorrentino M (1968) Positive and negative colloidal iron as cell surface electron stains. Lab Invest 18:63–71Google Scholar
  6. Lillie RD (1965) Methylation procedures. In: Histopathologic technic and practical histochemistry, 3rd edn. McGraw-Hill, New York, p 283Google Scholar
  7. Martinez-Palomo A, De Souza W, Gonzalez-Robles A (1976) Topographical differences in the distribution of surface coat components and intramembrane particles. A cytochemical and freeze-fracture study in culture forms of Trypanosoma cruzi. J Cell Biol 69:507–513Google Scholar
  8. Maurer A, Muhlethaler K (1982) Isolation and localization of plasma membrane-bound inventase in yeast (Saccharomyces cerevisiae). Eur J Cell Biol 216:219–227Google Scholar
  9. Murphy CR, Rogers AW (1981) Effects of ovarian hormones on cell membranes in the rat uterus. III. The surface carbohydrates at the apex of the luminal epithelium. Cell Biophys 3:305–320Google Scholar
  10. Murphy CR, Swift JG (1983) Relationships between intramembranous particles and surface coat carbohydrates in cells of a compact tissue. J Cell Sci (in press)Google Scholar
  11. Murphy CR, Swift JG, Mukherjee TM, Rogers AW (1982a) Loss of microvilli caused by glycerol treatment of uterine epithelial cells. Acta Anat 114:361–366Google Scholar
  12. Murphy CR, Swift JG, Mukherjee TM, Rogers AW (1982b) Fluidity of integral and peripheral membrane components in uterine epithelial cells. Micron 13:349–350Google Scholar
  13. Nicolson GL (1972) A rapid method for determining the topological distribution of anionic sites on membrane surfaces. J Supramol Struct 1:159–164Google Scholar
  14. Pinto da Silva P, Douglas SD, Branton D (1971) Localization of A antigen sites on human erythrocyte ghosts. Nature 232:194–196Google Scholar
  15. Pinto da Silva P, Martinez-Palomo A (1974) Induced redistribution of membrane particles, anionic sites and con A receptors in Entamoeba histolytica. Nature 249:170–171Google Scholar
  16. Pinto da Silva P, Nicolson GL (1974) Freeze-etch localization of concanavalin A receptors to the membrane intercalated particles of human erythrocyte ghost membranes. Biochim Biophys Acta 363:311–319Google Scholar
  17. Sandoz D, Boisvieux-Ulrich E, Chailley B (1979) Relationships beteen intramembrane particles and glycoconjugates in the ciliary membrane of quail oviduct. Biol Cell 36:267–280Google Scholar
  18. Schrevel J, Gros D, Monsigny M (1981) Cytochemistry of cell glycoconjugates. Prog Histochem Cytochem 14:1–269Google Scholar
  19. Tillack TW, Scott RE, Marchesi VT (1972) The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembrane particles. J Exp Med 135:1209–1227Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • C. R. Murphy
    • 1
  • S. Bradbury
    • 1
  1. 1.Department of Human AnatomyOxfordEngland

Personalised recommendations