Histochemistry

, Volume 80, Issue 1, pp 19–21 | Cite as

Ultrastructural localization of acetylcholinesterase activity by means of the electron dense precipitate derived from Koelle's cuprous thiocholine iodide by treatment with phosphomolybdic acid and osmium tetroxide

  • S. Tsuji
  • M. Fournier
Article

Summary

An osmium resistant, thermostable and electron dense precipitate was obtained from cuprous thiocholine iodide (Koelle's precipitate) by a joint interaction with phosphomolybdic acid and OsO4. No diffusion artifact due to the conversion of the primary precipitate to the secondary precipitate was observed, contrary to that seen after (NH4)2S or K3Fe+++ (CN)6 posttreatment of the cuprous thiocholine iodide. In addition, phosphomolybdic acid and OsO4 provided a counterstain effect on the ultrastructural background. By the present modification, Koelle's histochemical method becomes a useful cytochemical method for ultrastructural localization of acetylcholinesterase activity.

Keywords

Public Health Iodide Osmium Acetylcholinesterase Osmium Tetroxide 

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References

  1. Couteaux R (1963) The differentiation of synaptic areas. Proc R Soc (Lond) Sect B 158:457–480Google Scholar
  2. Couteaux R, Taxi J (1952) Recherches histochimiques sur la distribution des activités cholinestérasiques au niveau de la synapse myoneurale. Arch Anat Microsc Morphol Exp 41:352–392Google Scholar
  3. Gautron J (1982) Ultrastructural localization of acetylcholinesterase. A direct method for light and electron microscopy. Histochemistry 76:469–478Google Scholar
  4. Karnovsky HJ, Roots L (1964) A “direct coloring” thiocholine method for cholinesterase. J Histochem Cytochem 12:219–221Google Scholar
  5. Koelle GB, Friedenwald JS (1949) A histochemical method for localizing cholinesterase activity. Proc Soc Exp Biol Med 70:617–622Google Scholar
  6. Pearse AGV (1972) Histochemistry: Theoretical and applied, vol. 2, 3rd edn Churchill Livingstone, EdinburghGoogle Scholar
  7. Silver A (1974) The biology of cholinesterase. North-Holland, Elsevier, AmsterdamGoogle Scholar
  8. Souchay P, Tsuji S (1970) Contribution à l'étude de la réaction des sels de cuivre-thiocholine utilisée dans la détection histochimique des cholinestérases. Ann Histochim 15:263–271Google Scholar
  9. Tsuji S (1968) Sur la localisation des cholinestérases à l'aide d'iodure d'esters de thiocholine. CR Acad Sci (Paris) 267:801–803Google Scholar
  10. Tsuji S (1974) On the chemical basis of thiocholine methods for demonstration of acetylcholinesterase activities. Histochemistry 42:99–110Google Scholar
  11. Tsuji S, Alameddine HS, Nakanishi S, Ohoka T (1983) Molybdic and tungstic heteropolyanions for “ionic fixation” of acetylcholine in cholinergic motor nerve terminals. Histochemistry 77:51–56Google Scholar
  12. Tsuji S, Larabi Y (1983) A modification of thiocholine-ferricyanide method of Karnovsky and Roots for localization of acetylcholinesterase activity without interference by Koelle's copper thiocholine iodide precipitate. Histochemistry 78:317–323Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Tsuji
    • 1
  • M. Fournier
    • 2
  1. 1.Laboratoire de CytologieUniversité P. et M. CurieParis Cedex 05France
  2. 2.Laboratoire de Physico-chimie InorganiqueUniversité P. et M. CurieParis Cedex 05France

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