Summary
Estradiol was found to stimulate the activity of adenylate cyclase in the cervicovaginal epithelium of neonatal mice, demonstrated with adenylyl-imidodiphosphate and also with adenosine triphosphate (ATP) as substrate. Measures were taken to exclude interference by other ATP-degrading enzymes. The deposits of the reaction product, indicating enzyme activity, were localized on the plasma membrane of all sides of the epithelial cells. A similar distribution of enzyme activity was recorded in both controls and estradiol-stimulated animals with either of the two substrates; however, the deposits appeared most prominent after estradiol-treatment. In view of a previous report on increased activity of the cyclic AMP-degrading enzyme phosphodiesterase after estradiol-treatment, it was concluded that the parallel elevation in activity of both adenylate cyclase and cyclic AMP-phosphodiesterase indicates a role of cyclic AMP in the action mechanism of estradiol.
Similar content being viewed by others
References
Åbro, A., Kvinnsland, S.: Immunocytological studies on an estradiol sensitive antigen in the cervicovaginal epithelium of neonatal mice. Z. Zellforsch. 133, 559–569 (1972)
Barka, T., Van der Noen, H.: Adenylate cyclase activity in rat submandibular gland during postnatal development. Life Sci. 14, 267–280 (1974)
Cohen, K. L., Bitensky, M. W.: Inhibitory effects of alloxan on mammalian adenyl cyclase. J. Pharmacol. exp. Ther. 169, 80–86 (1969)
DePierre, J. W., Karnovsky, M. L.: Plasma membranes of mammalian cells. A review of methods for their characterization and isolation. J. Cell Biol. 56, 275–303 (1973)
Drummond, G. I., Duncan, L.: Adenyl cyclase in cardiac tissue. J. biol. Chem. 245, 976–983 (1970)
Dupont-Mairesse, N., Van Sande, J., Rooryck, J., Fastrez-Boute, A., Galand, P.: Mechanism of estrogen action-independence of several responses of the rat uterus from the early increase in adenosine 3′,5′-cyclic monophosphate. J. Steroid Biochemistry 5, 173–178 (1974)
Eide, A., Höisæter, P. Å., Kvinnsland, S.: Estradiol receptor in uterine tissue from neonatal mice. Influence by cyclic AMP. J. Steroid Biochemistry (in press)
Glick, N. B.: Inhibition of transport reactions. A. Inhibitors of ATPase: NaK-ATPase and related enzymic activities. In: Metabolic inhibitors (Hochster, R. M., Kates, M., Quastel, J. H., eds.), vol. 3, p. 1–45. London-New York: Academic Press 1972
Greengard, P., McAfee, D. A., Kebabian, J. W.: On the mechanism of action of cyclic AMP and its role in synaptic transmission. In: Advances in cyclic nucleotide research (Greengard, P., Robinson, G. A., Paoletti, R., eds.), vol. 1, p. 337–355. New York: Raven Press 1972
Higazi, M. G., Kvinnsland, S.: Oestradiol-17β: its influence on cyclic 3′,5′-adenosine monophosphate phosphodiesterase and 5′-nucleotidase in the cervicovaginal epithelium of neonatal mice. J. Reprod. Fertil. 36, 135–139 (1974)
Howell, S. L., Whitfield, M.: Cytochemical localization of adenyl cyclase activity in rat islets of Langerhans. J. Histochem. Cytochem. 20, 873–879 (1972)
Iqbal, S. J., Weakley, B. S.: The effects of different preparative procedures on the ultrastructure of the hamster ovary. I. Effects of various fixative solutions on ovarian oocytes and their granulosa cells. Histochemistry 38, 95–122 (1974)
Kanamori, T., Hayakawa, T., Nagatsu, T.: Adenosine 3′,5′-monophosphate-dependent protein kinase and amylase secretion from rat parotid gland. Biochem. biophys. Res. Commun. 57, 394–398 (1974)
Korenman, S. G., Sanborn, B. M., Bhalla, R. C.: Adenyl cyclase and the cyclic AMP responsive system in the uterus. In: Advances in experimental medicine and biology (O'Malley, B. W., Means, A. R., eds.), vol. 36, p. 241–261. London-New York: Plenum Press 1973
Kvinnsland, S.: Estradiol-17β, cyclic AMP and prostaglandins: In vivo and in vitro studies on the cervicovaginal epithelium from neonatal mice. Life Sci. 12, Part I, 373–384 (1973)
Kvinnsland, S., Åbro, A.: Cyclic AMP: its effect on an estrogen-sensitive antigen in organ cultures of the cervicovaginal epithelium from neonatal mice. Experientia (Basel) 28, 846–848 (1972)
Maurer, H. R.: Cleavage of ATP by mouse uterine chromatin after in vivo administration of oestradiol. Z. Naturforsch. 27b, 1267–1270 (1972)
Mietkiewski, K., Domka, F., Malendowicz, L., Malendowicz, J.: Studies on ATP hydrolysis in medium for histochemical demonstration of ATPase activity. Histochemie 24, 343–353 (1970)
Moses, H. L., Rosenthal, A. S.: Pitfalls in the use of lead ion for histochemical localization of nucleoside phosphatases. J. Histochem. Cytochem. 16, 530–539 (1968)
North, R. J.: The localization by electron microscopy of nucleoside phosphatase activity in guinea pig phagocytic cells. J. Ultrastruct. Res. 16, 83–95 (1966)
Øye, I., Sutherland, E. W.: The effect of epinephrine and other agents on adenyl cyclase in the cell membrane of avian erythrocytes. Biochim. biophys. Acta (Amst.) 127, 347–354 (1966)
Perkins, J. P.: Adenyl cyclase. In: Advances in cyclic nucleotide research (Greengard, P., Robison, G. A., eds.), vol. 3, p. 1–64. New York: Raven Press 1973
Reik, L., Petzold, G. L., Higgins, J. A., Greengard, P., Barrnett R. J.: Hormone-sensitive adenyl cyclase: cytochemical localization in rat liver. Science 168, 382–384 (1970)
Reimann, E. M., Walsh, D. A., Krebs, E. G.: Purification and properties of rabbit skeletal muscle adenosine 3′,5′-monophosphate-dependent protein kinases. J. biol. Chem. 246, 1986–1995 (1971)
Rodbell, M., Birnbaumer, L., Pohl, S. L., Krans, H. M. J.: The glucagon-sensitive adenyl cyclase system in plasma membranes of rat liver. V. An obligatory role of guanyl nucleotides in glucagon action. J. biol. Chem. 246, 1877–1882 (1971)
Sananes, N., Psychoyos, A.: Cytochemical localization of adenyl cyclase in the rat uterus. J. Reprod. Fertil. 38, 181–183 (1974)
Schmidt, M. J., Palmer, E. C., Dettbarn, W. D., Robison, G. A.: Cyclic AMP and adenyl cyclase in the developing rat brain. Develop. Psychobiology 3, 53–67 (1970)
Schulze, W., Krause, E. G., Wollenberger, A.: Cytochemical demonstration and localization of adenyl cyclase in skeletal and cardiac muscle. In: Advances in cyclic nucleotide research (Greengard, P., Robison, G. A., Paoletti, R., eds.), vol. 1, p. 249–260. New York: Raven Press 1972
Singhal, R. L.: Cyclic adenosine 3′,5′-monophosphate and estrogenic stimulation of uterine metabolism. In: Advances in pharmacology and chemotherapy (Garattini, S., Hawking, F., Goldin, A., Kopin, I. J., eds.), vol. 11, p. 99–150. London-New York: Academic Press 1973
Szego, C. M., Davis, J. S.: Adenosine 3′,5′-monophosphate in rat uterus: acute elevation by estrogen. Proc. nat. Acad. Sci. (Wash.) 58, 1711–1718 (1967)
Tu, J. C., Malhotra, S. K.: Histochemical localization of adenyl cyclase in the fungus Phycomyces blakesleeanus. J. Histochem. Cytochem. 21, 1041–1046 (1973)
Wachstein, M., Meisel, E.: Histochemistry of hepatic phosphatases at a physiological pH with special reference to the demonstration of bile canaliculi. Amer. J. clin. Path. 27, 13–23 (1957)
Wagner, R. C., Kreiner, P., Barrnett, R. J., Bitensky, M. W.: Biochemical characterization and cytochemical localization of catecholamine-sensitive adenylate cyclase in isolated capillary endothelium. Proc. nat. Acad. Sci. (Wash.) 69, 3175–3179 (1972)
Weiss, B., Crayton, J.: Gonadal hormones as regulators of pineal adenyl cyclase activity. Endocrinology 87, 527–533 (1970)
Yoshida, H., Nagai, K., Kamei, M., Nakagawa, Y.: Irreversible inactivation of (Na+−K+-dependent ATPase and K+-dependent phosphatase by fluoride. Biochim. biophys. Acta (Amst.) 150, 162–164 (1968)
Yount, R. G., Babcock, D., Ballantyne, W., Ojala, D.: Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P-N-P linkage. Biochemistry 10, 2484–2489 (1971)
Zor, U., Koch, J., Lamprecht, S. A., Ausher, J., Lindner, A. R.: Mechanism of oestradiol action on the rat uterus: independence of cyclic AMP, prostaglandin E2 and β-adrenergic mediation. J. Endocr. 58, 525–533 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Åbro, A., Kvinnsland, S. Adenylate cyclase in an estradiol sensitive tissue: A cytochemical study. Histochemistry 42, 333–344 (1974). https://doi.org/10.1007/BF00492681
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00492681