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Histochemistry

, Volume 42, Issue 4, pp 333–344 | Cite as

Adenylate cyclase in an estradiol sensitive tissue: A cytochemical study

  • Arnold Åbro
  • Stener Kvinnsland
Article

Summary

Estradiol was found to stimulate the activity of adenylate cyclase in the cervicovaginal epithelium of neonatal mice, demonstrated with adenylyl-imidodiphosphate and also with adenosine triphosphate (ATP) as substrate. Measures were taken to exclude interference by other ATP-degrading enzymes. The deposits of the reaction product, indicating enzyme activity, were localized on the plasma membrane of all sides of the epithelial cells. A similar distribution of enzyme activity was recorded in both controls and estradiol-stimulated animals with either of the two substrates; however, the deposits appeared most prominent after estradiol-treatment. In view of a previous report on increased activity of the cyclic AMP-degrading enzyme phosphodiesterase after estradiol-treatment, it was concluded that the parallel elevation in activity of both adenylate cyclase and cyclic AMP-phosphodiesterase indicates a role of cyclic AMP in the action mechanism of estradiol.

Keywords

Public Health Plasma Membrane Enzyme Activity Epithelial Cell Adenosine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Arnold Åbro
    • 1
  • Stener Kvinnsland
    • 1
  1. 1.Institute of AnatomyUniversity of BergenBergenNorway

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