Histochemistry

, Volume 42, Issue 3, pp 247–256 | Cite as

Relation between the nuclei and the nucleoli during cell differentiation in roots of Vicia faba volumetric and cytochemical analysis

  • Alain Bregnard
  • Fritz Ruch
Article

Summary

The behaviour of the nuclei and the nucleoli of roots of Vicia faba during cell differentiation was studied quantitatively. The relations between these cell constituents and the polyploidy was analysed. The study was made on isolated nuclei and nucleoli and on plastic sections. A method for the isolation of nuclei and nucleoli of secondary roots fixed in formol was modified and another developed for material fixed in ethanol/acetic acid mixture. The volumetric investigation showed that the nuclear volume increases while the nucleolar decreases during cell differentiation. The mean number of nucleoli decreases. In Vicia faba there is no relation between the ploidy and the volume of nuclei and nucleoli; the protein synthesis rate has an influence on the size of these organelles. Quantitative investigation has shown the proportionality of dry weight, DNA, total protein, histone, protein-bound lysine and arginine content of the nuclei and their ploidy. The same experiments made on nucleoli showed linear relation between their content and volume. The concentration of analysed substances in constant in nucleoli.

Keywords

Lysine Arginine Cell Differentiation Formol Linear Relation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Alain Bregnard
    • 1
  • Fritz Ruch
    • 1
  1. 1.Department of General BotanySwiss Federal Institute of TechnologyZürichSwitzerland

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