, Volume 42, Issue 3, pp 247–256 | Cite as

Relation between the nuclei and the nucleoli during cell differentiation in roots of Vicia faba volumetric and cytochemical analysis

  • Alain Bregnard
  • Fritz Ruch


The behaviour of the nuclei and the nucleoli of roots of Vicia faba during cell differentiation was studied quantitatively. The relations between these cell constituents and the polyploidy was analysed. The study was made on isolated nuclei and nucleoli and on plastic sections. A method for the isolation of nuclei and nucleoli of secondary roots fixed in formol was modified and another developed for material fixed in ethanol/acetic acid mixture. The volumetric investigation showed that the nuclear volume increases while the nucleolar decreases during cell differentiation. The mean number of nucleoli decreases. In Vicia faba there is no relation between the ploidy and the volume of nuclei and nucleoli; the protein synthesis rate has an influence on the size of these organelles. Quantitative investigation has shown the proportionality of dry weight, DNA, total protein, histone, protein-bound lysine and arginine content of the nuclei and their ploidy. The same experiments made on nucleoli showed linear relation between their content and volume. The concentration of analysed substances in constant in nucleoli.


Lysine Arginine Cell Differentiation Formol Linear Relation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Birnstiel, M. L., Chipchase, M. I. H., Flamm, W. G.: On the chemistry and organization of the nucleolar proteins. Biochem. biophys. Acta (Amst.) 87, 111–122 (1964)Google Scholar
  2. Bregnard, A.: Etude cytophotométrique des noyaux et des nucléoles des racines latérales de Vicia faba durant la différenciation cellulaire. Diss. No 4897 ETH, Zürich, 1972Google Scholar
  3. Busch, H., Smetana, K.: The nucleolus. New York: Acad. Press, 1970Google Scholar
  4. Deeley, E. M., Davies, H. G., Chayen, J.: The DNA content of cells in the root of Vicia faba. Expt. Cell Res. 12, 582–591 (1957)Google Scholar
  5. Heitz, E.: Nukleolen und Chromosomen in der Gattung Vicia. Planta, Arch. Wiss. Bot. 15, 495–505 (1931)Google Scholar
  6. Leemann, U., Ruch, F.: Cytophotometric determination of basic and total proteins with Sulfaflavine. J. Histochem. Cytochem., 20, 659–671 (1972)Google Scholar
  7. Leemann, U., Weiss, S., Schmutz, E.: A centrifugation technique for cytochemical preparations. J. Histochem. Cytochem. 12, 758–760 (1971)Google Scholar
  8. McLeish, J.: DNA in plant nucleoli. Nature (Lond.) 204, 36–39, 1964Google Scholar
  9. Müller, W.: Untersuchungen zur interferenzmikroskopischen Massenbestimmungen an inhomogenen Objekten. Diss. Nr 4272 ETH, Zürich, 1968Google Scholar
  10. Rees, H., Jones, R.N.: The origin of the wide species variation in nuclear DNA content. Int. Rev. Cytol. 32, 53–92 (1972)Google Scholar
  11. Rosselet, A.: Mikrofluorometrische Argininbestimmung. Z. wiss. Mikr. 68, 22–41 (1963)Google Scholar
  12. Rosselet, A., Ruch, F.: Cytophotometric determination of lysine with dansylchloride. J. Histochem. Cytochem., 16, 459–466 (1968)Google Scholar
  13. Ruch, F.: Determination of DNA content by microfluorometry. In: Introduction to quantitative cytochemistry, ed. Wied, G. L., p. 281–294. New York: Acad. Press Inc. 1966Google Scholar
  14. Ruch, F., Trapp, L.: Ein Mikroskopfluorometer mit Kurzzeit-Anregung und Schaltfolgesteuerung. Zeiss Informationen 81, 1973Google Scholar
  15. Sundermann, N., McLeish, J.: Nucleic acid content and concentration in root cells of higher plants. Expt. Cell Res. 24, 541–554, 1961Google Scholar
  16. Wallace, H.: Nucleolar growth and fusion during cellular differentiation. J. Morph. 112, 261–278, 1963Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Alain Bregnard
    • 1
  • Fritz Ruch
    • 1
  1. 1.Department of General BotanySwiss Federal Institute of TechnologyZürichSwitzerland

Personalised recommendations