, Volume 42, Issue 3, pp 233–237 | Cite as

Exogenous acetylcholinesterase as tracer for extracellular pathways in the brain

  • Georg W. Kreutzberg
  • Hisanobu Kaiya


Exogenous acetylcholinesterase from the electric eel has been used to trace extracellular pathways of the brain. After pretreatment with DFP, which inhibits irreversibly the endogenous AChE, the enzyme is injected into the brain tissue or the lateral ventricles. From the site of injection the enzyme spreads into the brain along extracellular pathway, so that the extracellular space can be demonstrated easily with this method. AChE shows special affinity towards basal laminae (basement membrane) constituents.


Public Health Brain Tissue Basement Membrane Extracellular Space Acetylcholinesterase 
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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Georg W. Kreutzberg
    • 1
  • Hisanobu Kaiya
    • 1
  1. 1.Max-Planck-Institut für PsychiatrieMünchen 40Federal Republic of Germany

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