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Histochemical localization of acetylcholinesterase in the cerebellum and optic tectum of four freshwater teleosts

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Summary

A histochemical study has been carried out on the localization of acetylcholinesterase (AChE) in the cerebellum and optic tectum of four species of freshwater teleosts. AChE distribution in the cerebellar cortex of teleosts shows differences among the species examined and, in the trout, also differences between different cerebellar areas. This uneven kind of enzyme distribution corresponds to a similar variety of AChE patterns noticed in other vertebrates, especially mammals. AChE distribution in the optic tectum shows a prevalent pattern characterized by precise laminar distribution of enzymatic activity which is alternatively strong, weak or absent in the different tectal layers. The results suggest that most of sensitive imput and many systems of stimuli propagation may be mediated by cholinergic mechanisms in the optic tectum of teleosts.

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References

  • Ariens Kappers, C. U., Huber, G. C., Crosby, E. C.: The comparative anatomy of the nervous system of vertebrates, including man. Vol. II. New York: Hafner 1936 (reprinted in 1960)

    Google Scholar 

  • Arvy, L.: Cerebellar enzymology. Int. Rev. Cytol. 20, 277–359 (1966)

    Google Scholar 

  • Baumgarten, H. G.: Biogenic monoamines in the cyclostome and lower vertebrate brain. Progr. Histochem. Cytochem. 4, 1–90 (1972)

    Google Scholar 

  • Boell, E. J., Greenfield, P., Shen, S. C.: Development of cholinesterase in the optic lobes of the frog (Rana pipiens). Exp. Zool. 129, 415–451 (1955)

    Google Scholar 

  • Capurro, S., Zaccheo, D., Viale, G.: Recherches histochimiques sur l'activité cholinesterasique de l'ecorce cerebelleuse. C. R. Ass. Anat. 46, 146–151 (1960)

    Google Scholar 

  • Cavanagh, J. B., Holland, P.: Cholinesterase in the chicken nervous system. Nature (Lond.) 190, 735–736 (1961)

    Google Scholar 

  • Ciani, F., Leghissa, S., Villani, L.: Ultrastructural organization of the optic tectum of Poecilia reticulata (teleosts). Riv. Biol. (1975, in press)

  • Contestabile, A.: Histochemical study on the distribution of some enzymic activities in the vagal and facial lobes of the goldfish, Carassius auratus. Histochemistry 44, 123–132 (1975a)

    Google Scholar 

  • Contestabile, A.: Histochemical localization of acetylcholinesterase in the cerebellum of some seawater teleosts. Brain Res. (1975b, in press)

  • Contestabile, A., Minelli, G., Ciani, F.: Enzymic activities in dissociated neurons differentiated in cultures in vitro. J. Neurochem. 20, 691–698 (1973)

    Google Scholar 

  • Csillick, B., Joo, F., Kasa, P., tomity, I., Kalman, Gy.: Development of acetylcholinesterase active structures in the rat archicerebellar cortex. Acta biol. Acad. Sci. hung. 15, 11–17 (1964)

    Google Scholar 

  • Crawford, J. M., Curtis, D. R., Vorhoeve, P. E., Wilson, V. J.: Acetylcholine sensitivity of cerebellar neurones in the cat. J. Physiol. (Lond.) 186, 139–165 (1966)

    Google Scholar 

  • Crook, J. C.: Acetylcholinesterase activity of capillary blood vessels in the central nervous system of the rabbit. Nature (Lond.) 199, 41–43 (1963)

    Google Scholar 

  • Filogamo, G.: Recherches experimentales sur l'activité des cholinesterase specificque et non specifique, dans le developpement du lobe optique du poulet. Arc. Biol. (Liège) 71, 159–195 (1960)

    Google Scholar 

  • Flumerfelt, B. A., Lewis, P. R., Gwyn, D. G.: Cholinesterase activity of capillaries in the rat brain. A light and electron microscopic study. Histochem. J. 5, 67–77 (1973)

    Google Scholar 

  • Friede, R. L.: Topographic brain chemistry. New York: Academic Press 1966

    Google Scholar 

  • Friede, R. L., Fleming, M. L.: A comparison of cholinesterase distribution in the cerebellum of several species. J. Neurochem. 11, 1–9 (1964)

    Google Scholar 

  • Gerebtzoff, M. A.: Recherches histochimiques sur les acetylcholine et choline esterase. I. Introduction et tecnique. Acta anat. (Basel) 19, 366–379 (1953)

    Google Scholar 

  • Gerebtzoff, M. A.: Cholinesterases. London: Pergamon Press 1959

    Google Scholar 

  • Hebb, C. O., Raktovic, D.: Choline acetylase in the evolution of the brain in vertebrates. In: Comparative neurochemistry (D. Richter, ed.), p. 347–354. London: Pergamon Press 1964

    Google Scholar 

  • Ieradi, L. A., Cataldi, E.: Localizzazione delle colinesterasi nel cervelletto di uccelli. Riv. Histochim. norm. pat. 10, 413–420 (1964)

    Google Scholar 

  • Ito, H.: Fine structure of the carp optic tectum. J. Hirnforsch. 12, 325–354 (1971)

    Google Scholar 

  • Karnovsky, M. J., Roots, L.: A “direct coloring” thiocholine method for cholinesterases. J. Histochem. Cytochem. 12, 219–221 (1964)

    Google Scholar 

  • Kasa, P.: Ultrastructural localization of choline acetyltransferase and acetylcholinesterase in central and peripheral nervous tissue. Progr. Brain Res. 34, 337–344 (1971)

    Google Scholar 

  • Kasa, P., Csillick, B., Joo, F., Knyar, E.: Histochemical and ultrastructural alterations in the isolated archicerebellum of the rat. J. Neurochem. 13, 173–178 (1966)

    Google Scholar 

  • Koelle, J. B.: The histochemical identification of acetylcholinesterase in cholinergic, adrenergic and sensory neurons. J. Pharmacol. exp. Ther. 114, 167–184 (1955)

    Google Scholar 

  • Kusunoki, T.: The chemoarchitectonics of the turtle brain. Yokoama med. Bull. 22, 1–29 (1971)

    Google Scholar 

  • Kusunoki, T., Masai, H.: Chemoarchitectonics in the central nervous system of goldfish. Arch. hist. jap. 27, 363–371 (1966)

    Google Scholar 

  • Kusunoki, T., Tsuda, Y., Takashima, F.: The chemoarchitectonics of the shark brain. J. Hirnforsch. 14, 13–26 (1973)

    Google Scholar 

  • Laufer, M., Vanegas, H.: The optic tectum of a perciform teleost. II. Fine structure. J. comp. Neurol. 154, 61–96 (1974a)

    Google Scholar 

  • Laufer, M., Vanegas, H.: The optic tectum of a perciform teleost. III. Electron microscopy of degenerating retino-tectal afferents. J. comp. Neurol. 154, 97–115 (1974b)

    Google Scholar 

  • Leghissa, S.: La structtura microscopica e la citoarchitettonica del tetto ottico dei pesci teleostei. Z. Anat. Entwickl.-Gesch. 118, 427–463 (1955)

    Google Scholar 

  • Leghissa, S., Contestabile, A., Poli, A.: Lo sviluppo della attività enzimatica nel tetto ottico di uccelli a prole atta (Coturnix). Atti Accad. Sci. Bologna, Ser. XII, 10, 26–32 (1973)

    Google Scholar 

  • McDonald, D. M., Rasmussen, C. L.: Ultrastructural characteristics of synaptic endings in the cochlear nucleus having acetylcholinesterase activity. Brain Res. 28, 1–18 (1971)

    Google Scholar 

  • Minelli, G.: Histochemical studies on certain enzymatic activities of the encephalon of Testudo graeca and Coturnix coturnix. Riv. Biol. 63, 61–86 (1970)

    Google Scholar 

  • Minelli, G., Ciani, F., Contestabile, A.: The occurrence of some enzymatic activities in the differentiation of nerve tissue of Gallus embryo in cultures in vitro. Histochemie 28, 160–169 (1971)

    Google Scholar 

  • Minelli, G., Quaglia, A.: The fine localization of acetylcholinesterase in the tectum mesencephali of Triturus cristatus carnifex. Riv. Biol. 61, 63–77 (1968)

    Google Scholar 

  • Navaratnam, V., Lewis, P. R.: Cholinesterase containing neurons in the spinal cord of the rat. Brain Res. 18, 411–425 (1970)

    Google Scholar 

  • Phillis, J. W.: Cholinergic mechanisms in the cerebellum. Brit. med. Bull. 21, 26–29 (1965)

    Google Scholar 

  • Phillis, J. W.: Acetylcholinesterase in the feline cerebellum. J. Neurochem. 15, 691–698 (1968)

    Google Scholar 

  • Sakharova, A. V.: Changes in cholinesterase in the cells of the cat cerebellum during development. Tsitologiya 8, 54–59 (1966)

    Google Scholar 

  • Schmatolla, E.: Dependence of tectal neuron differentiation on optic innervation in teleost fish. J. Embryol. exp. Morph. 27, 555–576 (1972)

    Google Scholar 

  • Sharma, S.: The retinal projections in the goldfish. An experimental study. Brain Res. 39, 213–223 (1972)

    Google Scholar 

  • Shen, S. C., Greenfield, P., Boell, E. J.: The distribution of cholinesterase in the frog brain. J. comp. Neurol. 102, 717–744 (1955)

    Google Scholar 

  • Shimizu, N., Seiji, I.: Electron microscopic histochemistry of acetylcholinesterase of the rat brain by Karnovsky method. Histochemie 6, 24–33 (1966)

    Google Scholar 

  • Silver, A.: Cholinesterases of the central nervous system with special reference to the cerebellum. Int. Rev. Neurobiol. 10, 57–109 (1967)

    Google Scholar 

  • Vanegas, H., Essayag-Millan, E., Laufer, M.: Laminar profile analysis of the tectal evoked response in the teleost Eugerres plumieri. Acta cient. venez. 22, 82–85 (1971)

    Google Scholar 

  • Vanegas, H., Laufer, M., Amat, J.: The optic tectum of a perciform teleost. I. General configuration and cytoarchitecture. J. comp. Neurol. 154, 43–60 (1974)

    Google Scholar 

  • Wachtler, K.: The distribution of acetylcholinesterase in the cyclostome brain. I. Lampetra planeri. Z. Zellforsch. 152, 259–270 (1974)

    Google Scholar 

  • Wawrzyniak, M.: Chemoarchitektonische Studien am Tectum opticum von Teleostiern unter normalen und experimentellen Bedingungen. Z. Zellforsch. 58, 234–264 (1962)

    Google Scholar 

  • Winkelmann, E., Winkelmann, L.: Vergleichend histologische Untersuchungen zur funktionellen Morphologie des Tectum opticum verschiedener Teleostier. J. Hirnforsch. 10, 1–16 (1968)

    Google Scholar 

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Authors and Affiliations

  1. Institute of Comparative Anatomy, University of Bologna, Bologna, Italy

    Antonio Contestabile & Nives Zannoni

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  1. Antonio Contestabile
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  2. Nives Zannoni
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Contestabile, A., Zannoni, N. Histochemical localization of acetylcholinesterase in the cerebellum and optic tectum of four freshwater teleosts. Histochemistry 45, 279–288 (1975). https://doi.org/10.1007/BF00492630

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