Summary
In situ hybridisation detection of mRNAs using riboprobes has become a widely used technique. However, the identification of cells producing closely-related yet distinct mRNAs is difficult with the usual size probes. More-over, it is not always easy to obtain the required cDNA essential for cRNA probe synthesis. To avoid these problems, we have used synthetic oligodeoxynucleotides to generate short, single stranded RNA probes (“oligo-riboprobes”). These probes can be labelled to very high (109 cpm/μg) specific activity and can be prepared for any published nucleotide sequence. We have used these probes to localise β (preprotachykinin) PPT mRNA producing neurons in rat hypothalamus and bowel. The results were compared to that obtained with cRNA probes generated from β preprotachykinin cDNA.
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Denny, P., Hamid, Q., Krause, J.E. et al. Oligo-riboprobes. Histochemistry 89, 481–483 (1988). https://doi.org/10.1007/BF00492605
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DOI: https://doi.org/10.1007/BF00492605