Histochemistry

, Volume 75, Issue 1, pp 53–65 | Cite as

No classical type IIB fibres in dog skeletal muscle

  • D. H. Snow
  • R. Billeter
  • F. Mascarello
  • E. Carpene
  • A. Rowlerson
  • E. Jenny
Article

Summary

To analyse the fibre type composition of adult dog skeletal muscle, enzyme histochemistry, immunohistochemistry for type I, IIA and IIB myosins, and peptide mapping of myosin heavy chains isolated from typed single fibres were combined. Subdivision of type II fibres into two main classes according to the activity of the m-ATPase after acidic and alkaline preincubation proved to be rather difficult and was only consistently achieved after a very careful adjustment of the systems used. One of these sub-classes of type II fibres stained more strongly for m-ATPase activity after acidic and alkaline preincubation, was oxidative-glycolytic and showed a strong reaction with an anti-type IIA myosin. The other one, however, although unreactive with anti-IIA myosin, was also oxidative-glycolytic, and only showed a faint reaction with an anti-type IIB myosin. Peptide mapping of the myosin heavy chains of typed single fibres revealed two populations of heavy chains among the type II fibre group. Thus, in dog muscle, we are confronted with the presence of two main classes of type II fibres, both oxidative-glycolytic, but differing in the structure of their myosin heavy chains. In contrast to some reports in the literature, no classical type IIB fibres could be detected.

Keywords

Peptide Heavy Chain Fibre Type Myosin Heavy Chain Single Fibre 

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References

  1. Aquin L, Banchero N (1981) The cytoarchitecture and capillary supply in the skeletal muscle of growing dogs. J Anat 132:341–356Google Scholar
  2. Billeter R, Weber H, Lutz H, Howald H, Eppenberger HM, Jenny E (1980) Myosin types in human skeletal muscle fibres. Histochemistry 65:249–259Google Scholar
  3. Billeter R, Heizmann CW, Howald H, Jenny E (1981) Analysis of myosin light and heavy chain types in single human skeletal muscle fibres. Eur J Biochem 116:389–395Google Scholar
  4. Braund KG, Hoff EJ, Richardson KEJ (1978) Histochemical identification of fibre types in canine skeletal muscle. Am J Vet Res 39:561–565Google Scholar
  5. Brooke MH, Kaiser KK (1970) Muscle fibre types: How many and what kind? Arch Neurol 23:369–379Google Scholar
  6. Bruggmann S, Jenny E (1975) The immunological specificity of myosins from cross-striated muscles as revealed by quantitative microcomplement fixation and enzyme inhibition by antisera. Biochim Biophys Acta 412:39–50Google Scholar
  7. Burke RE, Levine DN, Tsairis P, Zajac FE (1973) Physiological types and histochemical profiles in motor units of the cat gastrocnemius. J Physiol 234:723–748Google Scholar
  8. Carpenè E, Mascarello F, Veggetti A (1980) Masticatory muscle fibres in mammals: enzymatic pattern and immunohistochemical specificity for antisera to slow twitch myosin. J Muscle Res Cell Motil 1:481Google Scholar
  9. Carpenè E, Mascarello F, Veggetti A (1981) Purificazione della miosina dal massetere di pecora e di cavia e produzione di anticorpi specific. Basic Appl Histochem Suppl 25:48Google Scholar
  10. Cerretelli P, Piiper J, Mangili F, Ricci B (1964) Aerobic and anaerobic metabolism in exercising dogs. J Appl Physiol 19:25–28Google Scholar
  11. Dalla Libera L, Sartore S, Pierobon-Bormioli S, Schiaffino S (1980) Fast-white and fast-red isomyosins in guinea pig muscle. Biochem Biophys Res Commun 96:1662–1670Google Scholar
  12. Dill DB, Edwards HT, Talbott JH (1932) Studies in muscular activity. VII. Factors limiting the capacity for work. J Physiol Lond) 77:49–62Google Scholar
  13. Garnett RAF, O'Donovan MJ, Stephens JA, Taylor A (1978) Motor unit organization of human medial gastrocnemius. J Physiol (Lond) 287:33–43Google Scholar
  14. Gunn HM (1978) Differences in the histochemical properties of skeletal muscles of different breeds of horses and dogs. J Anat 127:615–634Google Scholar
  15. Guy PS, Snow DH (1981) Skeletal muscle fibre composition in the dog and its relationship to athletic ability. Res Vet Sci 31:244–248Google Scholar
  16. Kugelberg E (1976) Adaptive transformation of rat soleus motor units during growth. J Neurol Sci 27:269–289Google Scholar
  17. Lewis DM, Rowlerson A, Webb SN (1982) Motor units and immunohistochemistry of cat soleus muscle after long periods of cross-reinnervation. J Physiol 325:403–418Google Scholar
  18. Lojda Z, Gossrau R, Scheibler TH (1976) Enzymhistochemische Methoden. Springer, Berlin Heidelberg New York, pp 233–259Google Scholar
  19. Lutz H, Ermini M, Jenny E, Bruggmann S, Joris F, Weber E (1978) The size of the fibre populations in rabbit skeletal muscles as revealed by indirect immunofluorescence with antimyosin sera. Histochemistry 57:223–235Google Scholar
  20. Lutz H, Weber H, Billeter R, Jenny E (1979) Fast and slow myosin within single skeletal muscle fibres of adult rabbits. Nature 281:142–144Google Scholar
  21. Mascarello F, Veggetti A (1979) A comparative histochemical study of intrinsic laryngeal muscles of ungulates and carnivores. Basic Appl Histochemistry 23:103–125Google Scholar
  22. Maxwell LC, Barclay JK, Mohrmann DE, Faulkner JA (1977) Physiological characteristics of skeletal muscles of dogs and cats. Am J Physiol 133:C14-C18Google Scholar
  23. Nemeth P, Pette D (1981) Succinate dehydrogenase activity in fibres classified by myosin ATPase in three hind limb muscles of rat. J Physiol (Lond) 320:73–80Google Scholar
  24. Peter JB, Barnard RJ, Edgerton VR, Gillespie CA, Stempel KE (1972) Metabolic profiles of three fibre types of skeletal muscle in guinea pigs and rabbits. Biochemistry 11:2627–2633Google Scholar
  25. Reichmann H, Pette DA (in press) A comparative microphotometric study of succinate dehydrogenase activity levels in type I, IIA and IIB fibres of mammalian and human muscles. Histochemistry 74:27–41Google Scholar
  26. Rowlerson A, Pope B, Murray J, Whalen B, Weeds AG (1981) A novel myosin present in the cat jaw-closing muscles. J Muscle Res Cell Motil 2:415–438Google Scholar
  27. Seekerman HJ, Taylor RC, Maloiy GMD, Armstrong BR (1981) Design of the mammalian respiratory system. II. Measuring maximum aerobic capacity. Respir Physiol 44:11–23Google Scholar
  28. Snow DH, Billeter R, Jenny E (1981) Myosin types in equine skeletal muscle fibres. Res Vet Sci 30:381–382Google Scholar
  29. Taylor CR (1978) Why change gaits? Recruitment of muscles and muscle fibres as a function of speed and gait. Am Zool 18:153–161Google Scholar
  30. Trevino AS, Demaree RS, Sanders V, O'Donnell TA (1973) Needle biopsy of skeletal muscle in dogs: Light and electron microscopy of resting muscle. Am J Vet Res 34:507–514Google Scholar
  31. Van de Graaf KM, Frederick EC, Williamson RG, Goslow GE (1977) Motor units and fibre types of primary ankle extensors of the skunk (Mephitis mephitis). J Neurophysiol 40:1424–1431Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • D. H. Snow
    • 1
  • R. Billeter
    • 2
  • F. Mascarello
    • 3
  • E. Carpene
    • 4
  • A. Rowlerson
    • 2
  • E. Jenny
    • 2
  1. 1.Department of Veterinary PharmacologyUniversity of Glasgow, Veterinary SchoolGlasgowUK
  2. 2.Institute of Pharmacology and BiochemistryUniversity of ZürichZürichSwitzerland
  3. 3.Instituto di Anatomia degli Animali Domestici con Istologia col EmbriologiaUniversità di MilanoMilanoItaly
  4. 4.Istituto di Biochimica, Facoltà di Medicina VeterinariaUniversità di BolognaBolognaItaly

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