Histochemistry

, Volume 91, Issue 6, pp 527–530 | Cite as

Variability in gold bead density in cells

Quantitative immunocytochemistry
  • I. Hammel
  • M. Elmalek
  • M. Castel
  • M. Kalina
Article

Summary

Variability in gold bead distribution between individual cells was demonstrated in both pituitary melanotrophic cells immunocytochemically reacted for ACTH and in neurohypophysia terminals reacted for oxytocin-neurophysin. Gold beads were confined to the secretory granules compartment of both tissues. Density of gold beads in melanotrophic cells reacted for ACTH varied from 100–480 gold beads/μm2. A much narrower range of gold beads distribution (460–900 gold beads/μm2) was observed in axons of the neurohypophysis reacted with anti-oxytocin-neurophysin. These results indicate that the labeling density varies from cell to cell (as well as axon terminals) within morphologically homogeneous population. Thus, it may reflect certain physiological differences between cells. A suggestion is being made that mean gold bead density coefficient of variation should be calculated by comparison between individual cells.

Keywords

Public Health Gold Narrow Range Individual Cell Secretory Granule 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bendayan M (1984) Concentration of amylase along its secretory pathway in the pancreatic acinar cells as revealed by high resolution immunocytochemistry. Histochem J 16:85–108Google Scholar
  2. Bendayan M, Stephens H (1984) Double labelling cytochemistry applying the protein A-gold technique. In: Polak JM, Varndell IM (eds) Immunolabeling for electron microscopy. Elsevier, New York, pp 143–151Google Scholar
  3. Bendayan M, Zollinger M (1983) Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. J Histochem Cytochem 31:101–109Google Scholar
  4. Bendayan M, Bruneau A, Morisset J (1985) Morphometrical and immunocytochemical studies on rat pancreatic aciner cells under control and experimental conditions. Biol Cell 54:227–234Google Scholar
  5. Castel M, Morris JF, Whitnall MH, Sivan N (1986) Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by the use of immuno-gold technique. Cell Tissue Res 243:193–202Google Scholar
  6. Crine P, Gianoulakis C, Seidah NG, Gossard F, Pezalla PD, Lis M, Chretien M (1978) Biosynthesis of β-endorphin from β-lipo-protein and a larger molecular weight precursor in rat pars intermedia. Proc Natl Acad Sci USA 75:4719–4722Google Scholar
  7. De Mey J (1983) Colloidal gold probes in immunocytochemistry. In: Polak JM, van Noorden S (eds) Immunocytochemistry. PSG Wright, London 82–112Google Scholar
  8. Hammel I, Dvorak AM, Galli SJ (1987) Defective cytoplasmic granule formation abnormalities affecting tissue mast cells and pancreatic acinar cells of beige mice. Lab Invest 56:321–328Google Scholar
  9. Heap PF, Jones CW, Morris JF, Pickering BT (1975) Movement of Neurosecretory product through the anatomical compartments of the neural lobe of the pituitary gland. Cell Tissue Res. 156:483–497Google Scholar
  10. Kalina M, Elmalek M, Hammel I, (1988) Intragranular processing of pro-opiomelanocortin in the intermediate cells of the rat pituitary glands. A quantitative immunocytochemical approach. Histochemistry 89:193–198Google Scholar
  11. Mains RE, Eipper BA, Ling N (1977) Common precursor to corticotropins and endorphins. Proc Natl Acad Sci USA 74:3014–3018Google Scholar
  12. Mroz MA, Lechene C (1986) Pancreatic zymogen granules differ markedly in protein composition. Science 232:871–873Google Scholar
  13. Roth J (1982) The protein A-gold (pAg) technique — a qualitative and quantitative approach for antiger localization on thin sections. In: Bullock GR, Petrusz P (eds) Techniques in immunocytochemistry, vol 1. Academic Press, New York, pp 107–133Google Scholar
  14. Sokal RR, Rohlf FJ (1969) In biometry. Freeman, San FranciscoGoogle Scholar
  15. Varndell IM, Tapia FJ, Probert L, Buchman AMJ, Gu J, De May J, Bloom SR, Polak JM (1982) Immunogold staining procedure for the localization of regulatory peptides. Peptides 3:259–272Google Scholar
  16. Varndell IM, Sikri KL, Hennessy RJ, Kalina M, Goodman RH, Benoit R, Diani AR, Polak JM (1986) Mammalian somatostatin-containing D cells exhibits rat somatostatin cryptic peptide (RSCP) immunoreactivity: Electron microscopical study. Cell Tissue Res 246:196–204Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • I. Hammel
    • 1
  • M. Elmalek
    • 1
  • M. Castel
    • 3
  • M. Kalina
    • 2
  1. 1.Department of Pathology, Sackler School o MedicineTel-Aviv UniversityTel-AvivIsrael
  2. 2.Department of Histology and Cell Biology, Sackler School o MedicineTel-Aviv UniversityTel-AvivIsrael
  3. 3.Department of experimental ZoologyThe Hebrew UniversityJerusalemIsrael

Personalised recommendations