Summary
In this paper we describe the development of basement membrane (BM) reactive monoclonal antibodies (MA), by immunization of mice with intact denuded BM. The MA raised against denuded amniotic BM (clones 1052, 1053 and 1065) showed heterogeneous staining patterns. MA 1052 and 1053 reacted with epithelial BM of the epidermis and epidermal adnexa and furthermore with the epithelial alveolar BM in the lung and the superficial part of the epithelial BM in the gastrointestinal tract. MA 1065 showed immunoreactivity with the epithelial BM of epidermis and epidermal adnexa and the epithelial BM of trachea and oesophagus, and furthermore pericellular staining of the basal keratinocytes and basal corneal epithelial cells. MA 1087, raised against human glomerular BM, showed numunoreactivity with all BM, except the central epithelial BM in the cornea. The precise localization of the target epitopes in the BM was investigated on chemically cleaved human skin. Reactivity for the MA occurred predominantly in the BM lamina adherent to the dermis, suggesting that the target epitopes reside in the lamina densa and/or lamina fibroreticularis.
We furthermore examined the nature of the epitopes by preincubation of tissue sections with various enzymes prior to immunohistochemistry. The reactivity of the target epitopes was not affected by bacterial collagenase, but after various protease treatments the reactivity disappeared, suggesting that the epitopes are not localized on the triple helical part of collagenous proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bosman FT, Havenith MG, Cleutjens JPM (1985) Basement membranes in cancer. Ultrastruct Pathol 8:291–304
Bronckers AIJJ, Gay S, Lyaruu DM, Gay R, Miller EJ (1986) Localization of type V collagen with monoclonal antibodies in developing dental and peridental tissues of the rat and the hamster. Coll Relat Res 6:1–13
Butkowski RJ, Langeveld JPM, Wieslander J, Hamilton J, Hudson BG (1987) Localization of the Goodpasture epitope to a novel chain of basement membrane collagen. J Biol Chem 262:7874–7877
Cleutjens JPM, Havenith M, Beek C, Vallinga M, Bosman FT (1989) Basement membrane heterogeneity detected by monoclonal antibodies. Acta Histochem (in press)
Courtoy PJ, Timpl R, Farquhar MG (1982) Comparative distribution of laminin, type IV collagen, and fibronectin in the rat glomerulus. J Histochem Cytochem 30:874–886
Damjanov I (1987) Biology of disease: Lectin cytochemistry and histochemistry. Lab Invest 57:5–20
Farquhar MG (1981) The glomerular basement membrane. A selective macromolecular filter. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press, New York, pp 335–378
Fine JD (1985) Cicatricial pemphigoid, bullous pemphigoid and epidermolysis bullosa acquisita antigens. Differences in organ and species specificities and localization in chemically-separated human skin of three basement membrane antigens. Coll Relat Res 5:369–377
Fine JD, Redmar DA, Goodman AL (1987) Sequence of reconstitution of seven basement membrane components following split-thickness wound induction in primate skin. Arch Dermatol 123:1174–1178
Fitch JM, Linsenmayer TF (1983) Monoclonal antibody analysis of ocular basement membranes during development. Dev Biol 95:137–153
Foidart JM, Bere EW, Yaar M, Rennard SI, Gullino M, Martin GR, Katz SI (1980) Distribution and immunoelectron microscopic localization of laminin, a noncollagenous basement membrane glycoprotein. Lab Invest 42:336–342
Goodpasture EW (1919) The significance of certain pulmonary lesions in relation to the etiology of influenza. Am J Med Sci 158:864–870
Havenith MG, Cleutjens JPM, Beek C, van der Linden E, De Goeij AFPM, Bosman FT (1987) Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87:123–128
Hessle H, Sakai L, Hollister D, Burgeson R, Engvall E (1984) Basement membrane diversity detected by monoclonal antibodies. Differentiation 26:49–54
Hinsch K-D, Hansen D, Zimmermann A, Bruchhausen v. F (1988) Production and immunohistochemical characterization of monoclonal antibodies directed against renal basement membranes of rats. Histol Histopathol 3:315–322
Keene DR, Sakai LY, Lunstrum GP, Morris NP, Burgeson RE (1987) Type VII collagen forms an extended network of anchoring fibrils. J Cell Biol 104:611–621
Kleppel MM, Michael AF, Fish AJ (1986) Comparison of noncollagenous type IV collagen in the human glomerulus and EHS tumor. Biochim Biophys Acta 883:178–189
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Langeveld JPM, Veerkamp HH (1981) Chemical characterization of glomerular and tubular basement membranes of various mammalian species. Comp Biochem Physiol 688:31–40
Lee M-C, Damjanov I (1984) Lectin histochemistry of human placenta. Differentiation 28:123–128
Leu F, Engvall E, Damjanov I (1986) Heterogeneity of basement membranes of the human genitourinary tract revealed by sequential immunofluorescence staining with monoclonal antibodies to laminin. J Histochem Cytochem 34:483–489
Liotta LA, Lee CW, Morakis DJ (1980) New method for preparing large surfaces of intact human basement membrane for tumor invasion studies. Cancer Lett 11:141–152
Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:656–677
Ohno M, Martinez-Hernandez A, Ohno N, Kefalides NA (1983) Isolation of laminin from human placental basement membranes, amnion, chorion and chorionic microvessels. Biochem Biophys Res Commun 112:1091–1098
Sakai L, Keene D, Morris N, Burgeson R (1986) Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol 103:1577–1586
Verrando P, Ortonne J-P, Pautrat G, Hsi B-L, Yeh CJ (1986) Identification of a 37 kilodalton protein at the epidermal basement membrane by an antiserum to human amnion. J Invest Dermatol 87:190–196
Wewer U, Albrechtsen R, Manthorpe M, Varon S, Engvall E, Ruoslahti E (1983) Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis. J Biol Chem 258:12654–12660
Wieslander J, Barr JF, Butkowski RJ, Edwards SH, Bygren P, Heinegard D, Hudson BG (1984a) Goodpasture antigen of the glomerular basement membrane: localization to noncollagenous regions of type IV collagen. Immunology 81:3838–3842
Wieslander J, Bygren P, Heinegard D (1984b) Isolation of the specific glomerular basement membrane antigen involved in Goodpasture syndrome. Proc Natl Acad Sci USA 81:1544–1548
Wieslander J, Langeveld J, Butkowski R, Jodlowski M, Noelken M, Hudson BG (1985) Physical and immunochemical studies of the globular domain of type IV collagen. J Biol Chem 260:8564–8570
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cleutjens, J.P.M., Havenith, M.G., Vallinga, M. et al. Monoclonal antibodies to native basement membranes reveal heterogeneous immunoreactivity patterns. Histochemistry 92, 407–412 (1989). https://doi.org/10.1007/BF00492498
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00492498