, Volume 87, Issue 6, pp 619–622 | Cite as

Cytochemical investigation into the Ca-dependence of positive and negative hormonal imprinting in Tetrahymena

  • P. Kovács
  • G. Csaba


Insulin gives rise to positive imprinting in Tetrahymena pyriformis, but to negative imprinting in T. thermophila, as revealed by the respective increases and decreases in the insulin-binding capacity of these organisms observed during later interactions with this hormone. We found that changes in insulin-binding capacity exhibited parallelism with fluctuations of the levels of free, intracellular Ca2+ detectable by Quin-2 labeling. An exception was the second interaction of T. thermophila with insulin, which although showing a positive trend, produced a relatively small increase in the level of intracellular Ca2+. These observations suggest an interrelationship between hormone-binding capacity and the fluctuation of intracellular Ca2+ levels. Either hormone binding depends on the availability of Ca2+, or, alternatively, the latter depends on the binding capacity. Further studies are required to elucidate the true nature of this interdependence.


Public Health Binding Capacity Positive Trend True Nature Hormone Binding 
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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • P. Kovács
    • 1
  • G. Csaba
    • 1
  1. 1.Department of BiologySemmelweis University of MedicineBudapestHungary

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