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Histochemistry

, Volume 87, Issue 6, pp 619–622 | Cite as

Cytochemical investigation into the Ca-dependence of positive and negative hormonal imprinting in Tetrahymena

  • P. Kovács
  • G. Csaba
Article

Summary

Insulin gives rise to positive imprinting in Tetrahymena pyriformis, but to negative imprinting in T. thermophila, as revealed by the respective increases and decreases in the insulin-binding capacity of these organisms observed during later interactions with this hormone. We found that changes in insulin-binding capacity exhibited parallelism with fluctuations of the levels of free, intracellular Ca2+ detectable by Quin-2 labeling. An exception was the second interaction of T. thermophila with insulin, which although showing a positive trend, produced a relatively small increase in the level of intracellular Ca2+. These observations suggest an interrelationship between hormone-binding capacity and the fluctuation of intracellular Ca2+ levels. Either hormone binding depends on the availability of Ca2+, or, alternatively, the latter depends on the binding capacity. Further studies are required to elucidate the true nature of this interdependence.

Keywords

Public Health Binding Capacity Positive Trend True Nature Hormone Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • P. Kovács
    • 1
  • G. Csaba
    • 1
  1. 1.Department of BiologySemmelweis University of MedicineBudapestHungary

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