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Histochemical staining of exogenous chromium and iron with the catalytic oxidation of phenylamines

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Summary

A highly sensitive and specific method for staining exogenous chromium and iron in tissues is described. This method is superior to conventional complex-forming methods with regard to its sensitivity and specificity for these metals. The staining reaction is based on the metalcatalysed oxidation of phenylamines. Tissue sections were incubated in a medium containing hydrogen peroxide and phenylamines, p-phenylenediamine or phenylhydrazine. Results obtained from test-tube experiments concerning the catalytic activities of metals indicated that the staining reactions depends on the activities of metals in tissues.

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Authors and Affiliations

  1. First Department of Anatomy, St. Marianna, University School of Medicine, Sugao, Miyamae-ku, 213, Kawasaki, Japan

    Y. Sumi, Y. Koyama, T. Muraki & T. Suzuki

Authors
  1. Y. Sumi
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  2. Y. Koyama
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  3. T. Muraki
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  4. T. Suzuki
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Sumi, Y., Koyama, Y., Muraki, T. et al. Histochemical staining of exogenous chromium and iron with the catalytic oxidation of phenylamines. Histochemistry 83, 509–512 (1985). https://doi.org/10.1007/BF00492452

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  • DOI: https://doi.org/10.1007/BF00492452

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