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Histochemistry

, Volume 86, Issue 1, pp 35–41 | Cite as

Silver-enhanced colloidal gold probes as markers for scanning electron microscopy

  • L. Seopsi
  • L. -I. Larsson
  • L. Bastholm
  • M. Hartvig Nielsen
Article

Summary

Silver enlargement of small colloidal gold particles has been extensively used for the light microscopical visualization of gold probes. Very recently, a few investigators have employed physical developers in electron microscopy (both pre-embedding and on-grid staining methods). We now demonstrate that physical development of small colloidal gold particles advantageously can be exploited for labelling biological surfaces in scanning electron microscopy. This novel application of silver enhancement of colloidal gold particles is characterized by a high detection efficiency. Thus, specimens are labelled with small gold probes affording high immunocytochemical efficiency but being impossible to detect with the present scanning microscopes. These particles are subsequently scanning electronmicro-scopically visualized by silver enhancement.

Keywords

Gold Scanning Electron Microscopy Scanning Microscope Detection Efficiency Staining Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • L. Seopsi
    • 1
  • L. -I. Larsson
    • 1
  • L. Bastholm
    • 2
  • M. Hartvig Nielsen
    • 2
  1. 1.Unit of HistochemistryUniversity Institute of Pathological AnatomyCopenhagen ØDenmark
  2. 2.Department of Electron MicroscopyUniversity Institute of Pathological AnatomyCopenhagen ØDenmark

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