Histochemistry

, Volume 79, Issue 3, pp 335–344 | Cite as

Localization of lectin receptors on bovine epididymal spermatozoa using a colloidal gold technique

  • F. Sinowatz
  • A. E. Friess
Article

Summary

Mammalian spermatozoa that leave the testis are neither motile nor fertile. Maturation of spermatozoa occurs during epididymal transport. Among the changes during epididymal passage alterations of the surface properties of spermatozoa appear especially interesting. In this report we describe ultrastructural localization of Con A and WGA-binding sites on bovine spermatozoa from caput and cauda epididymides using a indirect lectin-horse-radish-peroxidase gold technique. With Con A the plasma-membrane covering the sperm head was heavily labelled with gold granules in caput as well as in cauda epididymal spermatozoa. A different distribution was observed for WGA-binding sites. The acrosomal region of caput and cauda spermatozoa was heavily labelled with gold, both in caput and cauda spermatozoa. The postacrosomal region was only sparsely marked in caput sperm whereas in sperm cells originating from the cauda a calix like membrane area displayed intense labelling. Some differences in the number of binding sites were also seen on sperm tails: those of caput spermatozoa show generally more Con A binding sites that those from cauda epididymal spermatozoa. No changes in the number of WGA-binding sites on sperm tails was observed during epididymal passage. The technical aspects of the indirect lectin-horse-radish-peroxidase gold technique are briefly discussed.

Keywords

Sperm Cell Colloidal Gold Membrane Area Sperm Head Epididymal Spermatozoon 

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References

  1. Barker LDS, Amann RP (1971) Epididymal physiology II. Immunofluorescent analyses of epithelial secretion and absorption, and of bovine sperm maturation. J Reprod Fert 26:319–332Google Scholar
  2. Bedford JM (1963) Morphological changes in rabbit spermatozoa during passage through the epididymis. J Reprod Fert 5:169–177Google Scholar
  3. Bedford JM (1975) Maturation, transport and fate of spermatozoa in the epididymis. In: Hamilton DW, Greep RO (eds) Handbook of physiology, Vol 5. Williams and Wilkins, Baltimore, pp 303–317Google Scholar
  4. Bedford JM, Cooper GW (1978) Membrane fusion events in the fertilization of vertebrate eggs. In: Poste G, Nicolson GL (eds) Membrane fusion, cell surface reviews, vol 5, North Holland, Amsterdam New York, pp 65–125Google Scholar
  5. Bedford JM, Nicander L (1971) Ultrastructural changes in the acrosome and sperm membrane during maturation of spermatozoa in the testis and epididymidis of the rabbit and monkey. J Anat 108:527–543Google Scholar
  6. Cooper GW, Bedford JM (1971) Acquisition of surface charge by the plasma membrane of mammalian spermatozoa during epididymal maturation. Anat Rec 169:300–301Google Scholar
  7. Edelman GM, Millette CF (1971) Molecular probes of spermatozoan structures. Proc Natl Acad Sci USA 68:2436–2440Google Scholar
  8. Flechon JE (1981) Ultrastructural and cytochemical modifications of rabbit spermatozoa during epididymal transport. In: Hafez ESE, Thibault CG (eds) The biology of spermatozoa. Transport, survival and fertilizing ability. Karger, Basel, pp 36–45Google Scholar
  9. Flechon JE, Morstin J (1975) Localisation des glycoproteines et des charges négatives et positives dans le revêtement de surface des spermatozoiides ejaculés de lapin et de taureau. Ann Histochim 20:291–300Google Scholar
  10. Gasic GJ, Berwick L, Sorretino M (1968) Positive and negative colloidal iron as cell surface electron stains. Lab Invest 18:63–71Google Scholar
  11. Geoghegan WD, Ackerman GA (1977) Adsorption of horseradish peroxidase, ovomucoid and antiimmunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin on cell surfaces at the electron microscopic level: a new method, theory and application. J Histochem Cytochem 25:1187–1200Google Scholar
  12. Gordon M, Dandekar PV, Bartoszewicz W (1975) The surface coat of epididymal, ejaculated and capacitated sperm. J Ultrastruct Res 50:199–200Google Scholar
  13. Horisberger M (1981) Colloidal gold: a cytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. Scanning Electron Microsc 2:9–31Google Scholar
  14. Horisberger M, Rosset J (1977) Colloidal gold, a useful marker for transmission and scanning electron microscopy. J Histochem Cytochem 25:295–305Google Scholar
  15. Killian GJ, Amann RP (1973) Immunoelectrophoretic characterization of fluid and sperm entering and leaving the bovine epididymis. Biol Reprod 9:489–499Google Scholar
  16. Koehler JK (1972) Human sperm head ultrastructure: a freeze-etching study. J Ultrastruct Res 39:520–539Google Scholar
  17. Koehler JK (1978) The mammalian sperm surface: studies with specific labelling techniques. Int Rev Cytol 54:73–10Google Scholar
  18. Nicolson GL, Yanagimachi R (1974) Mobility and the restriction of mobility of plasma membrane lectin-binding components. Science 184:1294–1296Google Scholar
  19. Nicolson GL, Usui N, Yanagimachi R, Yanagimachi H, Smith JR (1977) Lectin-binding sites on the plasma membranes of rabbit spermatozoa. Changes in surface receptors during epididymal maturation and after ejaculation. J Cell Biol 74:950–962Google Scholar
  20. Nicolson GL, Yanagimachi R (1979) Cell surface changes associated with epididymal maturation of mammalian spermatozoa. In: Fawcett DW, Bedford JM (eds) The spermatozoon. Urban and Schwarzenberg, Baltimore München, pp 187–194Google Scholar
  21. Olson GE, Hamilton DW (1976) Morphological changes in the midpiece of wooly oppossum spermatozoa during epididymal transit. Anat Rec 186:387–404Google Scholar
  22. Olson GE, Hamilton DW (1978)Characterization of the surface glycoproteins of rat spermatozoa. Biol Reprod 19:26–35Google Scholar
  23. Olson GE, Danzo BJ (1981) Surface changes in rat spermatozoa during epididymal transit. Biol Reprod 24:431–443Google Scholar
  24. O'Rand MG (1980) Antigens of spermatozoa and their environment. In: Dhindsa DS, Schumacher GFB (eds) Immunological aspects of infertility and fertility regulation. Elsevier North Holland, Amsterdam New York, pp 155–171Google Scholar
  25. O'Rand MG, Romrell LJ (1981) Localization of a single membrane autoantigen (RSA-1) on spermatogenic cells and spermatozoa. Dev Biol 84:326–331Google Scholar
  26. Plattner H (1971) Bull spermatozoa: an investigation by freeze-etching using widely different cryofixation procedures. J Submicrosc Cytol 3:19–32Google Scholar
  27. Voglmayr JK, Fairbanks G, Jackowitz MA, Colella JR (1980) Posttesticular developmental changes in the ram sperm cell surface and their relationship to luminal fluid proteins of the reproductive tract. Biol Reprod 22:655–667Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • F. Sinowatz
    • 1
  • A. E. Friess
    • 1
  1. 1.Institut für Anatomie der Universität RegensburgRegensburgGermany

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