, Volume 77, Issue 3, pp 353–363 | Cite as

Precursors of monoamine-storage organelles in developing megakaryocytes of the rat

  • T. Daimon
  • H. David


Identification and distribution of the precursors of aminestorage organelles in rat megakaryocytes during cell maturation were studied, using the uranaffin reaction for adenine nucleotide. The precursors of the amine-storage organelles appeared as 200–300 nm vesicles having an uranaffin electron dense granule, whereas they appeared as empty vesicles by conventional glutaraldehyde-OsO4 fixation. X-ray probe microanalysis confirmed the existence of U and P in the uranaffin reaction positive vesicles. The precursors appeared in the immature megakaryocytes, especially at the trans(mature) face of the Golgi apparatus, and rapidly increased in number in the maturing cells. The size of the uranaffin granules in the precursory organelles increased gradually during cell maturation and became almost equivalent to the dense body of blood platelets in the final stage of cell maturation.


Public Health Nucleotide Adenine Final Stage Golgi Apparatus 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • T. Daimon
    • 1
  • H. David
    • 2
  1. 1.Department of Anatomy, School of MedicineTeikyo UniversityTokyoJapan
  2. 2.Department of Electron Microscopy of the CharitéInstitute of Pathology of the Humboldt University BerlinBerlin

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