Abstract
Sera from 35 apparently normal humans, 37 compromised human patients, 30 hedgehogs and 30 sheep, were examined for precipitating antibodies to four opportunistic fungi — Absidia corymbifera, Aspergillus fumigatus, Candida albicans and Rhizopus arrhizus — using Counterimmunoelectrophoresis (CIE).
Precipitins to A. fumigatus were almost exclusively confined to specimens obtained from the compromised human group (51% of those examined) while Candida precipitating antibodies were detected in the sera of both normal (26%) and compromised (49%) humans and in 10% of the hedgehog specimens. Serum precipitins against the two phycomycetes included in the investigations were rare.
Because of the complexity of most fungal antigen extracts, it appears essential that sera be tested against a number of different antigen concentrations if CIE is to be used with confidence in fungal serology.
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References
Dee, T. H. 1975. Detection of Aspergillus fumigatus serum precipitins by counterimmunoelectrophoresis. J. clin. Microbiol. 2: 482–485.
Dee, T. H. and M. W. Rytel. 1975. Clinical application of counterimmunoelectrophoresis in detection of Candida serum precipitins. J. lab. clin. Med. 85: 161–166.
Faux, J. A., V. C. Stanley, H. R. Buckley and B. M. Partridge. 1975. A comparison of different extracts of Candida albicans in agar gel double diffusion techniques. J. immunol. Meth. 6: 235–247.
Flaherty, D. K., J. Barboriak, D. Emanuel, J. Fink, J. Marx, V. Moore, C. E. Reed and R. Roberts. 1974. Multilaboratory comparison of three immunodiffusion methods used for the detection of precipitating antibodies in hypersensitivity pneumonitis. J. lab. clin. Med. 84: 298–306.
Galussio, J. C., J. L. Fridman and R. Negroni. 1973. Rapid diagnosis of pulmonary mycosis by counterelectrophoresis. Mycopathologia mycol. appl. 51: 143–146.
Gordon, M. A., R. E. Almy, C. H. Greene and J. W. Fenton. 1971. Diagnostic mycoserology by immunoelectroosmophoresis: a general, rapid and sensitive microtechnic. Amer. J. clin. Path. 56: 471–474.
Gumma, S. A. and E. S. Mahgoub. 1975. Counterimmunoelectrophoresis in the diagnosis of mycetoma and its sensitivity as compared to immunodiffusion. Sabouraudia 13: 309–315.
Kleger, B. and L. Kaufman. 1973. Detection and identification of diagnostic Histoplasma capsulatum precipitates by counterelectrophoresis. Appl. Microbiol. 26: 231–238.
Kumar, U. N., B. Varkey and F. B. Landis. 1975. Allergic bronchopulmonary aspergillosis: an increasing clinical problem. Postgrad. Med. 58: 141–145.
Longbottom, J. L., J. Pepys and F. Temple Clive. 1964. Diagnostic precipitin test in aspergillus pulmonary mycetoma. Lancet 1: 588–589.
Longbottom, J. L. and J. Pepys. 1975. Diagnosis of Fungal Diseases. In Clinical Aspects of Immunology, 3rd ed. P. G. H. Gell, R. R. A. Coombs and P. J. Lachmann eds. Blackwell, Oxford.
Mackenzie, D. W. R. and C. M. Philpot. 1975. Counterimmunoelectrophoresis as a routine mycoserological procedure. Mycopathologia 57: 1–7.
Murray, I. G., H. R. Buckley and G. Turner. 1969. Serological evidence of Candida infection after open heart surgery. J. med. Microbiol. 2: 463–469.
Odds, F. C., E. G. V. Evans and K. T. Holland. 1975. Detection of Candida precipitins. A comparison of double diffusion and counterimmunoelectrophoresis. J. immunol. Meth. 7: 211–218.
Parsons, E. R. and E. Nassau. 1974. Candida serology in open-heart surgery. J. med. Microbiol. 7: 415–423.
Phanuphak, P., J. Salvaggio, J. Fink and P. Kohler. 1975. Incidence of serum precipitins against organicdust antigens in different populations by counterimmunoelectrophoresis. Chest 68: 753–758.
Remington, J. S., J. D. Gaines and M. A. Gilmer. 1972. Demonstration of Candida precipitins in human sera by counterimmunoelectrophoresis. Lancet 1: 413.
Shifrine, M., D. Pappagianis and J. Neves. 1975. Double electroimmunodiffusion: a rapid diagnostic test for canine coccidioidomycosis. Am. J. vet. Res. 36: 819–820.
Smith, J. M. B. 1968. Diseases of hedgehogs. Vet. Bull. 38: 425–430.
Smith, J. M. B. 1976. The investigation of opportunistic mycoses: A review. Pathology 8: 29–41.
Smith, J. M. B. 1976. Unpublished data.
Taschdjian, C. L., P. J. Kozinn, A. Okas, L. Caroline and M. A. Halle. 1967. Serodiagnosis of systemic candidiasis. J. infect. Dis. 117: 180–187.
Turner-Warwick, M., K. M. Citron, K. B. Carroll, B. E. Heard, D. N. Mitchell, J. Pepys, J. G. Scadding and C. A. Soutar. 1975. Immunologic lung disease due to aspergilli. Chest 68: 346–355.
Ward, G. W. and P. F. Kohler. 1973. Counterelectrophoresis as a rapid method for the detection of aspergillus precipitins in pulmonary disease. Chest 63:49S-51S.
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Smith, J.M.B. Counterimmunoelectrophoresis and opportunistic fungal infections. Mycopathologia 60, 99–104 (1977). https://doi.org/10.1007/BF00490379
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DOI: https://doi.org/10.1007/BF00490379