Histochemistry

, Volume 40, Issue 1, pp 31–37 | Cite as

Intracellular aluminum binding; A histochemical study

  • U. De Boni
  • J. W. Scott
  • D. R. Crapper
Article

Summary

Although aluminum is neurotoxic, the mechanisms and sites of action are unknown. Using the histochemical stains, morin and Solochrome Azurine, intracellular binding of aluminum was examined in brain tissues of animals with an aluminum induced encephalopathy, in human lymphocytes and in cells of plant meristems. Accumulation of aluminum occurred on chromatin of interphase nuclei and on chromosomes of mitotic cells. These findings suggest that neurofibrillary degeneration following the intracerebral injection of aluminum salts in experimental animals may be the results of interactions between aluminum and chromatin.

Keywords

Public Health Aluminum Experimental Animal Brain Tissue Morin 

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References

  1. Berlyne, G.M., Ben Ari, J., Knopf, E., Yagil, R., Weinberger, G., Danovitch, G.M.: Aluminum toxicity in rats. Lancet, March 11, (1972)Google Scholar
  2. Bowen, H.J.M.: In: Trace elements in biochemistry, p. 76. London-New York: Acad. Press. 1966Google Scholar
  3. Clifford, A.F.: In: Inorganic chemistry of qualitative analysis, 486–487. Englewood Cliffs, N.J.: Prentice Hall Inc. 1961Google Scholar
  4. Crapper, D.R.: Experimental neurofibrillary degeneration and altered electrical activity. Electroenceph. clin. Neurophysiol. 35, 575 (1973)Google Scholar
  5. Crapper, D.R., Dalton, A.J.: Alterations in short term retention, conditioned avoidance response acquisition and motivation following aluminum induced neurofibrillary degeneration. Physiol. Behav. 10, 925–933, 1973(a)Google Scholar
  6. Crapper, D.R., Dalton, A.J.: Aluminum induced neurofibrillary degeneration, brain electrical activity and alterations in acquisition and retention. Physiol. Behav. 10, 935–945, 1973(b)Google Scholar
  7. Crapper, D.R., Krishnan, S.S., Dalton, A.J.: Brain aluminum distribution in Alzheimer's disease and experimental neurofibrillary degeneration. Science 180, 511–513 (1973)Google Scholar
  8. Embree, L.J., Hamberger, A., Sjöstrand, J.: Quantitative cytochemical studies and histochemistry in experimental neurofibrillary degeneration. J. Neuropath. exp. Neurol. 26, 427–436 (1967)Google Scholar
  9. Exss, E., Summer, G.K.: Basic proteins in neurons containing fibrillary deposits. Brain Res. 49, 151–164 (1973)Google Scholar
  10. Gelfant, S.: Inhibition of cell division: a critical and experimental analysis. Rev. Cytol. 14, 1–39 (1963)Google Scholar
  11. Guilbault, G.G.: In: Practical fluorescence. Theory, methods and techniques, p. 223–230. New York: Marcel Dekker Inc. Publ. 1973Google Scholar
  12. Harris, B.A.: Ultrastructure and histochemistry of alumina in cortex. Exp. Neurol. 38, 33–63 (1973)Google Scholar
  13. Horecker, B.L., Stotz, E., Hogness, T.: The promoting effects of Al, Cr and rare earths in the succinic dehydrogenase cytochrome system. J. biol. Chem. 128, 251–256 (1939)Google Scholar
  14. Klatzo, I., Wiśniewski, H., Streicher, E.: Experimental production of neurofibrillary degeneration. J. Neropath. exp. Neurol. 24, 187–199 (1965)Google Scholar
  15. Kopeloff, L.M., Barrera, S.E., Kopeloff, N.: Recurrent convulsive seizures in animals produced by immunologic and chemical means. Amer. J. Psychiat. 98, 881–902 (1942)Google Scholar
  16. Patôcka, J.: The influence of Al+++ on cholinesterase and acetylcholinesterase activity. Acta biol. med. germ. 26, 845–846 (1971)Google Scholar
  17. Pearse, A.G.E.: Solochrome dyes in histochemistry with particular reference to nuclear staining. Acta histochem. (Jena) 4, 95–101 (1957)Google Scholar
  18. Pearse, A.G.E.: In: Histochemistry, 2nd edn. London: J. and A. Churchill Publ. 1960Google Scholar
  19. Terry, R.D., Peña, C.: Experimental production of neurofibrillary degeneration. (2) Electronmicroscopy, phosphatase histochemistry and electron probe analysis. J. Neuropath. exp. Neurol. 24, 200–210 (1965)Google Scholar
  20. Wiśniewski, H., Narkiewicz, O., Wiśniewska, K.: Topography and dynamics of neurofibrillar degeneration in aluminum encephalopathy. Acta neuropath. (Berl.) 9, 127–133 (1967)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • U. De Boni
    • 1
    • 2
  • J. W. Scott
    • 1
    • 2
  • D. R. Crapper
    • 1
    • 2
  1. 1.Department of PhysiologyUniversity of TorontoTorontoCanada
  2. 2.Department of MedicineUniversity of TorontoTorontoCanada

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