Summary
Neither rinsing nor short trypsin digestion did remove all steroid-3β-oldehydrogenase positive cells from the outer surface of teased seminiferous tubules of the rat testis. Unrinsed tubules contained great numbers of Leydig cells and similar cells were visible also after one or two weeks' culturing of the tubules. Leydig cells may greatly influence the results of studies concerning biosynthesis and metabolism of separated seminiferous tubules.
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Hovatta, O., Nykänen, M. & Kormano, M. Presence of steroid-3β-ol-dehydrogenase positive interstitial cells in teased rat seminiferous tubule preparations. Histochemistry 40, 25–30 (1974). https://doi.org/10.1007/BF00490270
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DOI: https://doi.org/10.1007/BF00490270