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Lectins as markers for eosinophilic leukocytes

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Summary

Lectin from Griffonia simplicifolia (GSA-I) and soybean (SBA) are reliable markers for human eosinophils. In this study we have shown that fluorochrome labeled GSA-I and SBA can be used for specific labeling of eosinophils in paraffin embedded tissue sections, in peripheral blood smears and in cell suspensions prepared for flow cytometry. These two lectins are useful diagnostic reagents which could be applied for further characterization of cytoplasmic components selectively found in human eosinophils.

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References

  • Ekblom P, Miettinen M, Rapola J, Foidart JM (1982) Demonstration of laminin, a basement membrane glycoprotein in routinely processed formalin fixed human tissues. Histochemistry 75:301–307

    Google Scholar 

  • Elias L, Van Epps DE (1984) Analysis of myelomonocytic leukemic differentiation by a cell surface marker panel including a fucose-binding lectin from Lotus tetragonolobus. Blood 63:1285–1290

    Google Scholar 

  • Gartner I (1980) Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll). Immunology 40:133–136

    Google Scholar 

  • Gleich GJ, Loegering DA (1984) Immunobiology of eosinophils. Annu Rev Immunol 2:429–445

    Google Scholar 

  • Goldstein IJ, Hayes CE (1978) The lectins: carbohydrate binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35:127–340

    Google Scholar 

  • Hsu S-M, Ree HJ (1983) Histochemical studies on lectin binding in reactive lymphoid tissues. J Histochem Cytochem 31:538–546

    Google Scholar 

  • Lee MC, Damjanov I (1984) Lectin histochemistry of human placenta. Differentiation 28:123–128

    Google Scholar 

  • Lee MC, Talerman A, Oosterhuis JW, Damjanov I (1985) Lectin histochemistry of classic and spermatocytic seminoma. Arch Pathol Lab Med 109:938–942

    Google Scholar 

  • Malin-Berdel J, Valet G, Thiel E, Forrester JA, Gurtler L (1984) Flow cytometric analysis of the binding of elevent lectins to human T- and B-cells and to human T- and B-cell lines. Cytometry 5:204–209

    Google Scholar 

  • Nicola NA, Morstyn G, Metcalf D (1980) Lectin receptors on human blood and bone marrow cells and their use in cell separation. Blood Cells 6:563–579

    Google Scholar 

  • Peters MS, Rodriguez M, Gleich GJ (1986) Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy. Lab Invest 54:656–662

    Google Scholar 

  • Peters MS, Schroeter AL, Kephart GM, Gleich GJ (1983) Localization of eosinophil granule major basic protein in chronic urticaria. J Invest Dermatol 81:39–42

    Google Scholar 

  • Pinkus GS, Said JW (1986) Leu-MI Immunoreactivity in nonhematopoietic neoplasms and myeloproliferative disorders: An immunoperoxidase study of paraffin sections. Am J Clin Pathol 85:278–282

    Google Scholar 

  • Raghuprasad PK (1982) A rapid simple method of basophil purification by density centrifugation on Percoll. J Immunol 129:2128–2123

    Google Scholar 

  • Robak T, Goldman JM (1985) Monoclonal antibodies reacting with myeloid cells. Br J Haematol 61:1–9

    Google Scholar 

  • Saito H, Yamada K, Breard J, Yoshie O, Mathe G (1986) A monoclonal antibody reactive with human eosinophils. Blood 67:50–55

    Google Scholar 

  • Strauchen JA (1982) Fluoresceinated lectins as probe for cell surface changes associated with lymphocyte transformation. AM J Hematol 12:227–232

    Google Scholar 

  • Tai P-C, Spry CJF, Peterson C, Venge P, Olsson I (1984) Monoclonal antibodies distinguish between storage and secreted form of eosinophil cationic protein. Nature 309:182–184

    Google Scholar 

  • Warnke RA, Gatter KC, Falini B, et al. (1983) Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 309:1275–1281

    Google Scholar 

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Lee, M.C., Turcinov, D. & Damjanov, I. Lectins as markers for eosinophilic leukocytes. Histochemistry 86, 269–273 (1987). https://doi.org/10.1007/BF00490257

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  • DOI: https://doi.org/10.1007/BF00490257

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