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Histochemistry

, Volume 92, Issue 2, pp 137–140 | Cite as

An electron spin resonance (ESR) study of free radicals formed from hematoxylin on tissue sections after blueing (alkalization)

  • G. Elek
  • A. Rockenbauer
  • L. Jókay
Article
  • 22 Downloads

Summary

A free radical signal of 12 G width and g=2.0045 can be observed in hematoxylin stained tissue blocks and sections. The amount of paramagnetic centres in stained specimens is significantly larger than in unstained ones. After alkalization simultaneously with the colour change the former free radical is detectable in hemalum precipitate and on stained paper strips. After solution of the stain in dioxane and alkalization, a well resolved hyperfine structure could be seen which could be assigned to three different radicals with the same g value as observed in the rigid matrix (tissue and paper). Quantitative evaluation of free radical concentration is also carried out for tissue sections.

Keywords

Public Health Free Radical Electron Spin Resonance Hematoxylin Tissue Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Boon ME, Drijver SJ (1986) Routine cytological staining techniques. Macmillan, London, pp 45, 60–63, and 125Google Scholar
  2. Clark G (1975) Absorbances of hematoxylin solutions at varying pH levels. Acta Histochem 54:78–83Google Scholar
  3. Dobbs AJ (1974) Factors of free radicals in fluid solution. In: Norman ROC (ed) Electron spin resonance, vol 2. The Chemical Society, London, pp 281–294Google Scholar
  4. Elek G, Rockenbauer A, Magyar É, Jòkay L (1987) A study of free radicals in paraffin embedded and deparaffinized human heart muscle tissue using ESR. Histochemistry 87:499–504Google Scholar
  5. Gabe M (1976) Histological techniques. Masson-Springer, New York Paris Heidelberg Berlin, p 196Google Scholar
  6. Geräte Information: EPR-Tomographie. Akademie der Wissenschaften der DDR, Zentrum für wissenschaftlichen Geräten, DDR-1199 Berlin, Rudower-Chaussee 6Google Scholar
  7. Gill GW, Frost JK, Miller KA (1974) A new formula for a half oxidized hematoxylin. Acta Cytol 18:300–311Google Scholar
  8. Imes NK, Sanders DC, Crane CR, Clark G (1969) Assaying actual hematein content of commercial hematoxylins and hemateins. Stain Technol 44:167–172Google Scholar
  9. Palmer RW, Lillie RD (1965) Spectroscopic and staining studies of the ripening and overripening of aluminium hematoxylins. Histochemie 5:44–54Google Scholar
  10. Pryor WA (1976) Free radicals in biology, vol 1. Academic Press, New York LondonGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • G. Elek
    • 1
  • A. Rockenbauer
    • 2
  • L. Jókay
    • 2
  1. 1.Department of Pathology of Railway HospitalBudapestHungary
  2. 2.Central Research Institute for Chemistry of the Hungarian Academy of SciencesBudapestHungary

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