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Histochemistry

, Volume 38, Issue 1, pp 57–70 | Cite as

Die Feinstruktur der Gallenblase und der Gallengänge

V. Histochemische Lokalisierung von Mukosubstanzen im menschlichen Gallenblasenepithel
  • L. Luciano
  • E. Reale
  • C. Wolpers
Article

Zusammenfassung

In den Hauptzellen des menschlichen Gallenblasenepithels (3 Cholesterose-Fälle) wurden Mukosubstanzen lichtmikroskopisch mit Hilfe der PAS- und Alcianblau-Reaktionen, elektronenmikroskopisch unter Anwendung der PA-TCH-Silberproteinat-und Ferrihydrosol-Methoden dargestellt.

Die PAS-Reaktion ist im Golgi-Komplex, in den Sekretgranula und auf den Antennulae microvillares positiv, ebenso die Alcianblau-Reaktion (bei pH=1,2) in den Sekretgranula und auf den Antennulae microvillares. Nach Methylierung wird die Alcianblau-Reaktion negativ.

Silberpartikel (PA-TCH-Silberproteinat-Methode) lassen sich in Übergangsvesikeln, Golgi-Komplex, Sekretionsgranula, dichten Körpern und Antennulae microvillares nachweisen; dabei zeigt der Golgi-Komplex deutlich eine Polarität. Außerdem ist diese Reaktion in Vesikeln und Tubuli im apikalen Anteil der Zelle auffallend. Wird die TCH-Behandlung unterlassen, so bleibt an den obengenannten Stellen die Reaktion negativ.

Eisen-Partikel (Ferrihydrosol-Methode) liegen erst in den innersten Sacculi des Golgi-Komplexes; Sekretionsgranula, dichte Körper, Vesikel und Tubuli im apikalen Anteil der Zelle sowie Antennulae microvillares zeigen ebenfalls eine positive Reaktion, jedoch nicht nach vorausgehender Methylierung der Schnitte.

Die Ergebnisse zeigen, daß vermutlich die histochemisch dargestellten Mukosubstanzen saure (sulfatierte) Glykoproteine enthalten.

On the fine structure of the gall bladder and biliary ducts

Summary

In three cases of cholesterosis the localization of mucosubstances in the principal cells of the human gallbladder was studied with the light microscope using the PAS-and the Alcian blue methods, and with the electron microscope applying the PA-TCH-silverproteinate and colloidal iron methods.

The Golgi complex was positive only with PAS, whereas secretory granules and antennulae microvillares stained with both PAS and Alcian blue (pH 1.2). After drastic methylation the Alcian blue reaction remained negative.

Silver particles (PA-TCH-silverproteinate method) could be observed in the transition elements between rough surfaced endoplasmic reticulum and Golgi complex, Golgi vesicles and saccules, secretory granules, dense bodies and antennulae microvillares. The Golgi complex displayed a distinct polarity. A positive reaction was observed also in vesicles and tubules located at the apex in the cells. After omission of TCH-treatment, a negative reaction in all these components was obtained.

In the Golgi complex iron particles (colloidal iron oxide method) could be seen only in the innermost saccules; secretory granules, dense bodies, vesicles and tubules of the apical portion of the cells as well as antennulae microvillares exhibited again a positive reaction, provided that no methylation was performed prior to staining.

Our histochemical results indicate that the mucosubstances of the human gall bladder epithelium probably contain acid (sulfated) glycoproteins.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • L. Luciano
    • 1
  • E. Reale
    • 1
  • C. Wolpers
    • 1
    • 2
  1. 1.Anatomisches Institut, Abteilung ElektronenmikroskopieMedizinische Hochschule HannoverHannoverBundesrepublik Deutschland
  2. 2.LübeckBundesrepublick Deutschland

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