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Die Feinstruktur der Gallenblase und der Gallengänge

On the fine structure of the gall bladder and biliary ducts

V. Histochemische Lokalisierung von Mukosubstanzen im menschlichen Gallenblasenepithel

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Summary

In three cases of cholesterosis the localization of mucosubstances in the principal cells of the human gallbladder was studied with the light microscope using the PAS-and the Alcian blue methods, and with the electron microscope applying the PA-TCH-silverproteinate and colloidal iron methods.

The Golgi complex was positive only with PAS, whereas secretory granules and antennulae microvillares stained with both PAS and Alcian blue (pH 1.2). After drastic methylation the Alcian blue reaction remained negative.

Silver particles (PA-TCH-silverproteinate method) could be observed in the transition elements between rough surfaced endoplasmic reticulum and Golgi complex, Golgi vesicles and saccules, secretory granules, dense bodies and antennulae microvillares. The Golgi complex displayed a distinct polarity. A positive reaction was observed also in vesicles and tubules located at the apex in the cells. After omission of TCH-treatment, a negative reaction in all these components was obtained.

In the Golgi complex iron particles (colloidal iron oxide method) could be seen only in the innermost saccules; secretory granules, dense bodies, vesicles and tubules of the apical portion of the cells as well as antennulae microvillares exhibited again a positive reaction, provided that no methylation was performed prior to staining.

Our histochemical results indicate that the mucosubstances of the human gall bladder epithelium probably contain acid (sulfated) glycoproteins.

Zusammenfassung

In den Hauptzellen des menschlichen Gallenblasenepithels (3 Cholesterose-Fälle) wurden Mukosubstanzen lichtmikroskopisch mit Hilfe der PAS- und Alcianblau-Reaktionen, elektronenmikroskopisch unter Anwendung der PA-TCH-Silberproteinat-und Ferrihydrosol-Methoden dargestellt.

Die PAS-Reaktion ist im Golgi-Komplex, in den Sekretgranula und auf den Antennulae microvillares positiv, ebenso die Alcianblau-Reaktion (bei pH=1,2) in den Sekretgranula und auf den Antennulae microvillares. Nach Methylierung wird die Alcianblau-Reaktion negativ.

Silberpartikel (PA-TCH-Silberproteinat-Methode) lassen sich in Übergangsvesikeln, Golgi-Komplex, Sekretionsgranula, dichten Körpern und Antennulae microvillares nachweisen; dabei zeigt der Golgi-Komplex deutlich eine Polarität. Außerdem ist diese Reaktion in Vesikeln und Tubuli im apikalen Anteil der Zelle auffallend. Wird die TCH-Behandlung unterlassen, so bleibt an den obengenannten Stellen die Reaktion negativ.

Eisen-Partikel (Ferrihydrosol-Methode) liegen erst in den innersten Sacculi des Golgi-Komplexes; Sekretionsgranula, dichte Körper, Vesikel und Tubuli im apikalen Anteil der Zelle sowie Antennulae microvillares zeigen ebenfalls eine positive Reaktion, jedoch nicht nach vorausgehender Methylierung der Schnitte.

Die Ergebnisse zeigen, daß vermutlich die histochemisch dargestellten Mukosubstanzen saure (sulfatierte) Glykoproteine enthalten.

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Literatur

  • Aschoff, L., Bacmeister, A.: Die Cholelithiasis. Jena: Gustav Fischer 1909

    Google Scholar 

  • Beams, H. W., Kessel, R. G.: The Golgi apparatus, structure and function. Int. Rev. Cytol. 23, 209–276 (1968)

    Google Scholar 

  • Bennett, G.: Migration of glycoprotein from Golgi apparatus to cell coat in the columnar cells of the duodenal epithelium. J. Cell Biol. 45, 668–673 (1970)

    Google Scholar 

  • Bennett, G., Leblond, C. P.: Formation of cell coat material for the whole surface of columnar cells in the rat small intestine, as visualized by radioautography with L-fucose-3H. J. Cell Biol. 46, 409–416 (1970)

    Google Scholar 

  • Berlin, J. D.: The localization of acid mucopolysaccharides in the Golgi complex of intestinal goblet cells. J. Cell Biol. 32, 760–766 (1967)

    Google Scholar 

  • Delaquerrière, L., Tremblay, G., Riopelle, J.: Argentaffine cells in chronic cholecystitis. Arch. Path. 74, 142–151 (1962)

    Google Scholar 

  • Drochmans, P.: Morphologie du glycogène. Etude au microscope électronique de colorations négatives du glycogène particulaire. J. Ultrastruct. Res. 6, 141–163 (1962)

    Google Scholar 

  • Eglitis, J. A., Hayes, E. R.: Free surface of the gall bladder epithelium with the light microscope. Anat. Rec. 140, 61–69 (1961)

    Google Scholar 

  • Elfving, G.: Crypts and ducts in the gallbladder wall. Acta path. microbiol. scand., Suppl. 135, 49, 5–43 (1960)

    Google Scholar 

  • Esterly, J. R., Spicer, S. S.: Mucin histochemistry of human gallbladder: changes in adenocarcinoma, cystic fibrosis, and cholecystitis. J. nat. Cancer Inst. 40, 1–12 (1968)

    Google Scholar 

  • Evett, R. D., Higgins, J. A., Brown, A. L., Jr.: The fine structure of normal mucosa in human gall bladder. Gastroenterology 47, 49–60 (1964)

    Google Scholar 

  • Favard, P.: The Golgi apparatus. In: Handbook of molecular cytology, Lima de Faria, A. (ed.), p. 1130–1155. Amsterdam: North Holland Publishing Co. 1969

    Google Scholar 

  • Häkkinen, I., Laitio, M.: Epithelial glycoproteins of human gallbladder. Arch. Path. 90, 137–147 (1970)

    Google Scholar 

  • Hardin, J. H., Spicer, S. S.: Ultrastructural localization of dialyzed iron-reactive mucosubstance in rabbit heterophils, basophils, and eosinophils. J. Cell Biol. 48, 368–386 (1971)

    Google Scholar 

  • Hayward, A. F.: The structure of gallbladder epithelium. Int. Rev. gen. exp. Zool. 3, 205–239 (1968)

    Google Scholar 

  • Hayward, A. F., Freston, J. W., Bouchier, I. A. D.: Changes in the ultrastructure of gall bladder epithelium in rabbits with experimental gallstones. Gut 9, 550–556 (1968)

    Google Scholar 

  • Ito, S.: The enteric surface coat on cat intestinal microvilli. J. Cell Biol. 27, 475–491 (1965)

    Google Scholar 

  • Ito, S.: Structure and function of the glycocalyx. Fed. Proc. 28, 12–25 (1969)

    Google Scholar 

  • Ito, S., Revel, J.-P.: Autoradiographic studies of the enteric surface coat. In: Gastrointestinal radiation injury, Sullivan, M. F. (ed.), p. 27–41. Amsterdam: Excerpta Med. Found. 1968

    Google Scholar 

  • Jahn, K.: Über histochemische Aldehydreaktionen nach Fixation mit Glutaraldehyd. Acta histochem. (Jena) 39, 298–301 (1971)

    Google Scholar 

  • Jennings, M. A., Florey, H. W.: Autoradiographic observations on the mucous cells of the stomach and intestine. Quart. J. exp. Physiol. 41, 131–152 (1956)

    Google Scholar 

  • Kantor, T. G., Schubert, M.: Method for the desulfation of chondroitin sulfate. J. Amer. chem. Soc. 79, 152–153 (1957)

    Google Scholar 

  • Laitio, M., Nevalainen, T.: Scanning and transmission electron microscope observations on human gallbladder epithelium. I. Adult structure. Z. Anat. Entwickl.-Gesch. 136, 319–325 (1972)

    Google Scholar 

  • Lane, N., Caro, L., Otero-Vilardebó, L. R., Godman, G. C.: On the site of sulfatation in colonic goblet cells. J. Cell Biol. 21, 339–351 (1964)

    Google Scholar 

  • Lev, R., Spicer, S. S.: Specific staining of sulphate groups with alcian blue at low pH. J. Histochem. Cytochem. 12, 309 (1964)

    Google Scholar 

  • Lev, R., Spicer, S. S.: A histochemical comparison of human epithelial mucins in normal and in hypersecretory states including pancreatic cystic fibrosis. Amer. J. Path. 46, 23–47 (1965)

    Google Scholar 

  • Luciano, L., Wermbter, G., Reale, E.: Die Feinstruktur der Gallenblase und der Gallengänge. III. Beobachtungen an gefriergeätzten Präparaten der Gallenblase der Maus unter besonderer Berücksichtigung der dichten Körper und des Verbindungskomplexes. Cytobiologie 7, 76–88 (1973)

    Google Scholar 

  • Luciano, L., Wolpers, C.: Die Feinstruktur der Gallenblase und der Gallengänge. IV. Die Cholesterose der menschlichen Gallenblasenschleimhaut. Virchows Arch. Abt. B Zellpath. 14, 147–158 (1973)

    Google Scholar 

  • Mayor, H. D., Hampton, J. C., Rosario, B.: A simple method for removing the resin from epoxy-embedded tissue. J. Cell Biol. 9, 909–910 (1961)

    Google Scholar 

  • Mollenhauer, H.H., Whaley, W.G.: An observation on the functioning of the Golgi apparatus. J. Cell Biol. 17, 222–225 (1963)

    Google Scholar 

  • Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963)

    Google Scholar 

  • Morré, D.J., Mollenhauer, H.H., Bracker, C.E.: Origin and continuity of Golgi apparatus. In: Origin and continuity of cell organelles, Reinert, J. and Ursprung, H. (eds.), p. 82–126. Berlin-Heidelberg-New York: Springer 1971

    Google Scholar 

  • Mowry, R.W.: Improved procedure for the staining of acidic polysaccharides by Müller's colloidal (hydrous) ferric oxide and its combination with the Feulgen and the periodic acid-Schiff reactions. Lab. Invest. 7, 566–576 (1958)

    Google Scholar 

  • Müller, G.: Über eine Vereinfachung der Reaktion nach Hale (1946). Acta histochem. (Jena) 2, 68–70 (1955)

    Google Scholar 

  • Neutra, M., Leblond, C.P.: The Golgi apparatus. Sci. Amer. 220, 100–107 (1969)

    Google Scholar 

  • Novikoff, A.B.: Lysosomes and related particles. In: The cell, Brachet, J. and A.E. Mirsky (eds.), vol. II, p. 423–488. New York: Academic Press 1961

    Google Scholar 

  • Novikoff, P.M., Novikoff, A.B., Quintana, N., Hauw, J.-J.: Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry. J. Cell Biol. 50, 859–886 (1971)

    Google Scholar 

  • Pearse, A.G.E.: Histochemistry, theoretical and applied, vol. I. London: J. & A. Churchill 1968

    Google Scholar 

  • Pfuhl, W.: Die Gallenblase und die extrahepatischen Gallengänge. In: Handbuch der mikroskopischen Anatomie des Menschen hrsg. von W.v. Möllendorff, Bd. 5, S. 426–462. Berlin: Springer 1932

    Google Scholar 

  • Pigman, W., Tsuiki, S.: The nature of the epithelial mucins. Int. dent. J. 9, 502–516 (1959)

    Google Scholar 

  • Rambourg, A.: Morphological and histochemical aspects of glycoproteins at the surface of animal cells. Int. Rev. Cytol. 31, 57–63 (1971)

    Google Scholar 

  • Rambourg, A., Chretien, M.: Emploi de coupes épaisses pour l'étude de l'appareil de Golgi en microscopie électronique. In: Microscopie électronique, Favard, P. (ed.), vol. III, p. 83–84. Paris: Soc. Franç. Micr. El. 1970

    Google Scholar 

  • Rambourg, A., Hernandez, W., Leblond, C.P.: Detection of complex carbohydrates in the Golgi apparatus of rat cells. J. Cell Biol. 40, 395–414 (1969)

    Google Scholar 

  • Rambourg, A., Leblond, C.P.: Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cell in the rat. J. Cell Biol. 32, 27–53 (1967)

    Google Scholar 

  • Revel, J.-P., Ito, S.: The surface components of cells. In: The specifity of cell surfaces, Davis, B.D. and Warren, L. (eds.), p. 211–234. Englewood Cliffs, N.J.: Prentice-Hall 1967

    Google Scholar 

  • Scott, J.E., Mowry, R.W.: Alcian blue—A consumers guide. J. Histochem. Cytochem. 18, 842 (1970)

    Google Scholar 

  • Spicer, S.S., Hardin, J.H.: Ultrastructure, cytochemistry, and function of neutrophil leukocyte granules. Lab. Invest. 20, 488–497 (1969)

    Google Scholar 

  • Spicer, S.S., Leppi, T.J., Stoward, P.J.: Suggestions for a histochemical terminology of carbohydrate-rich tissue components. J. Histochem. Cytochem. 13, 599–603 (1965)

    Google Scholar 

  • Spicer, S.S., Staley, M.W., Wetzel, M.G., Wetzel, B.K.: Acid mucosubstance and basic protein in mouse Paneth cells. J. Histochem. Cytochem. 15, 225–242 (1967)

    Google Scholar 

  • Spiro, R.G.: Glycoproteins. Ann. Rev. Biochem. 39, 599–638 (1970)

    Google Scholar 

  • Thiéry, J.-P.: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopie 6, 987–1018 (1967)

    Google Scholar 

  • Thiéry, J.-P.: Role de l'appareil de Golgi dans la synthèse des mucopolysaccharides, étude cytochimique. I. Mise en évidence de mucopolysaccharides dans les vésicules de transition entre l'ergastoplasme et l'appareil de Golgi. J. Microscopie 8, 689–708 (1969)

    Google Scholar 

  • Venable, J.H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407 (1965)

    Google Scholar 

  • Wallraff, J.: Gallengangsystem, Gallenblase und Galle. In: Handbuch der mikroskopischen Anatomie, hrsg. von, W. Bargmann, Bd. V/4, S. 277–363. Berlin-Heidelberg-New York: Springer 1969

    Google Scholar 

  • Watson, M.L.: Staining of tissue section for ellectron microscopy with heavy metals. J. biophys. biochem. Cytol. 4, 475–478 (1958)

    Google Scholar 

  • Wetzel, M.G., Wetzel, B.K., Spicer, S.S.: Ultrastructural localization of acid mucosubstances in the mouse colon with iron-containing stains. J. Cell Biol. 30, 299–315 (1966)

    Google Scholar 

  • Wolf-Heidegger, G., Stäubli, W., Hess, R.: Zur Ultrastruktur und Histochemie der Gallenblasenschleimhaut des Menschen und der Katze. Acta anat. (Basel) 62, 606–618 (1965)

    Google Scholar 

  • Yamada, K., Hoshino, M.: Morphochemical analysis of epithelial mucosubstances in the gall bladder to the fowl (Gallus domesticus). Histochemie 29, 120–128 (1972)

    Google Scholar 

  • Zeigel, R.F., Dalton, A.J.: Speculations based on the morphology of the Golgi systems in several types of protein-secreting cells. J. Cell Biol. 15, 45–54 (1962)

    Google Scholar 

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Authors and Affiliations

  1. Anatomisches Institut, Abteilung Elektronenmikroskopie, Medizinische Hochschule Hannover, Hannover, Bundesrepublik Deutschland

    L. Luciano, E. Reale & C. Wolpers

  2. Kronsforder Allee 3b, D-2400, Lübeck, Bundesrepublick Deutschland

    C. Wolpers

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  1. L. Luciano
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  2. E. Reale
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Luciano, L., Reale, E. & Wolpers, C. Die Feinstruktur der Gallenblase und der Gallengänge. Histochemistry 38, 57–70 (1974). https://doi.org/10.1007/BF00490220

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