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Histochemistry

, Volume 38, Issue 1, pp 35–41 | Cite as

The immunofluorescent demonstration of cathepsin B1 in tissue sections

  • B. Sylvén
  • O. Snellman
Article

Summary

A direct immunohistochemical method of high specificity is presented for the demonstration of sites of cathepsin B1. Antisera were raised in rabbits against pure enzyme preparations. The specific antibodies were subsequently isolated and labelled according to methods previously published. Sections from tissues specially rich in this enzyme like liver, kidney, intestinal epithelia and the active macrophages of talcum granulomas were studied. The results suggest a localization of this enzyme mainly to lysosomal organelles and phagocytic vacuoles, which are known from chemical data to contain this enzyme. The prerequisite for a positive tissue reaction is that the enzyme must be present in a form available for antigen-antibody interaction.

Keywords

Public Health Tissue Section Specific Antibody High Specificity Enzyme Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • B. Sylvén
    • 1
    • 2
    • 3
  • O. Snellman
    • 1
    • 2
  1. 1.The Cancer Research Division of RadiumhemmetKarolinska InstituteStockholmSweden
  2. 2.the Cancer Research Division, Institute of PathologyUniversity of ZürichZürichSwitzerland
  3. 3.Abteilung für KrebsforschungInstitut für Pathologische AnatomieZürichSwitzerland

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