Histochemistry

, Volume 43, Issue 4, pp 333–348 | Cite as

Aldehyde bisulfite-toluidine blue (ABT) staining as a topo-optical reaction for demonstration of linear order of vicinal OH groups in biological structures

  • Gy. Romhányi
  • Gy. Deák
  • J. Fischer
Article

Summary

The aldehyde-bisulfite-toluidine blue reaction followed by poststaining stabilization with potassium ferricyanide (ABT) is described as a topo-optical, oriented staining reaction of the vicinal OH groups of complex carbohydrates in biological structures such as polysaccharides, glycoproteins and glycolipids.

The birefringence as induced by the oriented dye binding as a result of ABT is indicative of linear order of the vicinal OH groups and, in turn, provides information on the ultrastructural pattern of carbohydrate moieties in biological substances, which pattern is often not demonstrable by other ultrastructural methods.

The possibilities of this new approach to the ultrastructural analysis of complex carbohydrates with ABT in a great number of biological substances is demonstrated and its practical value in histopathology discussed.

Keywords

Public Health Potassium Carbohydrate Aldehyde Polysaccharide 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Gy. Romhányi
    • 1
  • Gy. Deák
    • 1
  • J. Fischer
    • 1
  1. 1.Institute of Pathological AnatomyUniversity Medical SchoolPécsHungary

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