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Cytochemical localization of cholinesterase activity at the giant synapse of the squid

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Summary

The giant synapse of squid stellate ganglion is a chemical synapse where the transmitter substance is not known. The components of the ACh-system are present in squid nervous tissue in large quantities. However externally applied cholinergic drugs have no effect on junctional transmission. Using the Copper thiocholine method for electron microscopic cytochemistry the reaction product was found at the axolemmal surface, in the cisternae of the endoplasmic reticulum of neurons and occasionally between the infoldings of the sheat cells surounding the axons. Abundant deposits of end product are observed in the extracellular space in the proximity to junctional region. However, the localization of the cytochemical end product at the junctional region proper was observed frequently, but not consistently.

Radiometric measurements of enzyme activity have revealed that neither specific inhibitors nor specific substrates generaly used for differentiation of cholinesterases in mammalian nervous tissue can be employed for differentiation of squid enzymes. Considering the permeability barriers imposed for external acetylcholine by cytoplasmic processes and the high enzyme activity of structures surrounding the giant synapse, the possibility that acetylcholine may still be a candidate for the missing transmitter is discussed.

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References

  • Bryant, S. H., Brzin, M.: Cholinesterase activity of isolated giant synapses. J. Cell Physiol. 68, 107–108 (1966)

    Google Scholar 

  • Brzin, M., Dettbarn, W.-D., Rosenberg, P., Nachmansohn, D.: Cholinesterase activity per unit surface area of conducting membranes. J. Cell Biol. 26, 353–364 (1965)

    Google Scholar 

  • Brzin, M., Tennyson, V. M., Duffy, P. E.: Acetylcholinesterase in frog sympathetic and dorsal root ganglia. A study by electron microscope cytochemistry and microgasometric analysis with the magnetic diver. J. Cell Biol. 31, 215–242 (1966)

    Google Scholar 

  • Bulock, T. H., Hagiwara, S.: Intracellular recording from the giant synapse of the squid. J. gen. Physiol. 40, 565–577 (1957)

    Google Scholar 

  • Bulock, T. H., Nachmansohn, D.: Cholinesterase in primitive nervous systems. J. cell. comp. Physiol. 20, 239–242 (1942)

    Google Scholar 

  • Castejon, O. J., Villegas, C. M.: Fine structure of the synaptic contacts in the stellate ganglion of the squid. J. Ultrastruct. Res. 10, 585–598 (1964)

    Google Scholar 

  • Florey, E.: Comparative physiology: transmittersubstances. Ann. Rev. Physiol. 23, 501–528 (1961)

    Google Scholar 

  • Hagiwara, S., Tasaki, I.: A study on the mechanism of impulse transmission across the giant synapse of the squid. J. Physiol. (Lond.) 143, 114–137 (1958)

    Google Scholar 

  • Hama, K.: Some observations on the fine structure of the giant synapse in the stellate ganglion of the squid. Z. Zellforsch. 56, 437–444 (1962)

    Google Scholar 

  • Koelle, G. B., Friedenwald, J. S.: A histochemical method for localizing cholinesterase activity. Proc. Soc. exp. Biol. (N. Y.) 70, 617–622 (1949)

    Google Scholar 

  • de Lorenzo, A. J. D., Brzin, M., Dettbarn, W.-D.: Fine structure and organization of nerve fibers and giant axons in Homarus americanus. J. Ultrastruct. Res. 24, 367–384 (1968)

    Google Scholar 

  • Nachmansohn, D.: Proteins in excitable membranes. Science 168, 1059–1066 (1970)

    Google Scholar 

  • Rosenberg, P., Dettbarn, W. D., Brzin, M.: Acetylcholine and choline acetylase in squid giant axon, ganglia and retina. Nature (Lond.) 210, 858–859 (1966)

    Google Scholar 

  • Smith, D. S., Treherne, J. E.: The electron microscopic localization of cholinesterase activity in the central nervous system of an insect, Periplaneta americana L. J. Cell Biol. 26, 445–465 (1965)

    Google Scholar 

  • Villegas, G. M., Villegas, J.: Acetylcholinesterase localization in the giant nerve fiber of the squid. J. Ultrastruct. Res. 46, 149–163 (1974)

    Google Scholar 

  • Webb, G. D., Dettbarn, W.-D., Brzin, M.: Biochemical and pharmacological aspects of the synapses of the squid stellate ganglion. Biochem. Pharmacol. 15, 1813–1819

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Authors and Affiliations

  1. Institute of Pathophysiology, University of Ljubljana, Yugoslavia

    Miro Brzin, Virginia M. Tennyson & Wolf-Dietrich Dettbarn

  2. Departments of Neurology and Pathology (div. of Neuropathology), Columbia University, New York

    Miro Brzin, Virginia M. Tennyson & Wolf-Dietrich Dettbarn

  3. Department of Pharmacology, Vanderbilt University, Nashville

    Miro Brzin, Virginia M. Tennyson & Wolf-Dietrich Dettbarn

Authors
  1. Miro Brzin
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  2. Virginia M. Tennyson
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  3. Wolf-Dietrich Dettbarn
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Supported in part by Boris Kidrič Foundation, Ljubljana, Yugoslavia, and NIH Grant No. 02-008-1.

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Brzin, M., Tennyson, V.M. & Dettbarn, WD. Cytochemical localization of cholinesterase activity at the giant synapse of the squid. Histochemistry 43, 305–311 (1975). https://doi.org/10.1007/BF00490189

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  • DOI: https://doi.org/10.1007/BF00490189

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