Histochemistry

, Volume 88, Issue 1, pp 61–64 | Cite as

Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies

  • M. Geffard
  • S. Tuffet
  • N. Mons
  • J. -L. Chagnaud
Article

Summary

Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei. In this staining procedure, the first antigen was localized using 3,3′-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphtol basic dye (2-NBD) method. The two antigens were localized in different cells or structures. No overlap of the staining was observed, thus indicating that dopamine is not localized with serotonin, 5-methoxytryptamine or tryptamine.

Keywords

Public Health Dopamine Monoclonal Antibody Serotonin Specific Antibody 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • M. Geffard
    • 1
    • 2
  • S. Tuffet
    • 1
    • 2
  • N. Mons
    • 1
    • 2
  • J. -L. Chagnaud
    • 1
    • 2
  1. 1.Laboratoire de NeuroimmunologieInstitut de Biochimie CellulaireBordeaux CédexFrance
  2. 2.Neurochimie du CNRSUniversité de Bordeaux IIBordeaux CédexFrance

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