Summary
Several segments of the gastrointestinal tract of the white-belly opossum Didelphis albiventris were investigated immunocytochemically for the occurrence of polypeptide YY (PYY) and enteroglucagon (GLU). PYY- and GLU-immunoreactive cells were observed in the lower part of the ileum, cecum and colon. These cells were seen to emit cytoplasmic basal processes to the neighbouring cells with a number of them reaching the glandular lumen via apical cytoplasmic process. GLU-immunoreactive cells were also present in the oxyntic mucosae and in the pancreatic duct. Staining of consecutive sections for the two polypeptides, respectively, reaveled the coexistence of immunoreactivity for PYY and GLU in the same cell type.
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Ali-Rachedi A, Varndell IM, Adrian TE, Gapp TA, Van Noorden S, Bloom SR, Polak JM (1984) Peptide YY (PYY) immunoreactivity is costored with glucagon related immunoreactants in endocrine cells of the gut and pancreas. Histochemistry 80:487–491
Alumets J, Sundler F, Häkanson R (1977) Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res 185:465–479
Bloom SR, Polak JM (1982) Enteroglucagon and the gut hormone profile of intestinal adaptation. In: Robinson JWL, Dowling RH, Riecken EO (eds) Mechanism of intestinal adaptation. MTP Press, Lancaster Boston, pp 189–199
Böttcher G, Sjölund K, Ekblad E, Håkanson R, Schwartz TW, Sundler F (1984) Coexistence of peptide YY and glicentin immunoreactivity in endocrine cells of the gut. Regul Pept 8:261–266
Calingason NY, Kitamura N, Yamada J, Oomori Y, Yamashita T (1984) Immunocytochemical study of the gastroenteropancreatic endocrine cells of the sheep. Acta Anat 118:171–180
El-Salhy M, Wilander E, Grimelius L, Terenius L, Lundberg JM, Tatemoto K (1982) The distribution of polypeptide YY (PYY)- and pancreatic polypeptide (PP)-immunoreactive cells in the domestic fowl. Histochemistry 75:25–30
El-Salhy M, Wilander E, Juntti-Berggren L, Grimelius L (1983a) The distribution and ontogeny of polypeptide YY (PYY)- and pancreatic polypeptide (PP)-immunoreactive cells in the gastrointestinal tract of the rat. Histochemistry 78:53–60
El-Salhy M, Grimelius L, Wilander E, Ryberrg B, Terenius L, Lundberg JM, Tatemoto K (1983b) Immunocytochemical identification of polypeptide YY (PYY) cells in the human gastrointestinal tract. Histochemistry 77:15–23
Helmstaedter V, Taugner Ch, Feurle GE, Forssmann WG (1977) Localization of neurotensin-immunoreactive cells in the small intestine of man and various mammals. Histochemistry 53:35–41
Johnson CR (1977) New aspects of the trophic action of gastrointestinal hormones. Gastroenterology 72:788–792
Kitamura N, Yamada J, Yamashita T, Yanaihara N (1982) Endocrine cells in the gastrointestinal tract of the cat. Biomed Res 3:612–622
Kitamura N, Yamada J, Calingason Y, Iamashita T (1984) Immunocytochemical distribution of endocrine cells in the gastrointestinal tract of the horse. Equine Vet J 16:103–107
Krause WJ, Yamada J, Cutts JH (1985) Quantitative distribution of enteroendocrine cells in the gastrointestinal tract of the adult opossum, Didelphis virginiana. J Anat 140:591–605
Krause WJ, Yamada J, Cutts JH (1986) Enteroendocrine cells in the developing opossum stomach. J Anat 148:47–57
Larsson LI, Holst J, Håkanson R, Sundler F (1975) Distribution and properties of glucagon immunoreactivity in the digestive tract of various mammals: An immunocytochemical and immunochemical study. Histochemistry 44:281–290
Larsson LI, Sundler F, Alumets J, Håkanson R, Schaffalitzky de Muckadell OB, Fahrenkrug J (1977) Distribution, ontogeny and ultrastructure of the mammalian secretin cell. Cell Tissue Res 181:361–368
Lehy T, Gres L, Ferreira de Castro E (1979) Quantitation of gastrin and somatostatin cell population in the antral mucosa of the rat. Comparative distribution and evolution through different life stages. Cell Tissue Res 198:325–333
Lundberg JM, Tatemoto K, Terenius L, Hellström PM, Mutt V, Hökfelt T (1982) Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc Natl Acad Sci USA 79:4471–4475
Polak JM, Pearse AGE, Bloom SR, Buchan AMJ, Rayford PL, Thompson JC (1975) Identification of cholecystokinin-secreting cells. Lancet 2:1016–1018
Ravazzola M, Siperstein A, Moddly AJ, Sundby F, Jacobsen H, Orci L (1979) Glicentin immunoreactive cells. Their relationship to glucagon-producing cells. Endocrinology 63:784–800
Simpson GG (1945) Principles of classification and a classification of mammals. Bull Am Mus Nat Hist 85:1–350
Sternberger LA (1986) Immunocytochemistry, 3rd edn. John Wiley & Sons, New York, pp 90–209
Tatemoto K, Mutt V (1980) Isolation of two novel candidate hormones using a chemical method for finding naturally occurring polypeptides. Nature 285:417–418
Tatemoto K (1982) Isolation and characterization of peptide YY (PYY), a candidate gut hormone that inhibits pancreatic exocrine secretion. Proc Natl Acad Sci USA 79:2414–2518
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Work supported by grants from FINEP and CNPq (Brazil)
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Barbosa, A.J.A., Nogueira, J.C., Penna, F.J. et al. Distribution of enteroglucagon- and polypeptide YY-immunoreactive cells in the gastrointestinal tract of the white-belly opossum (Didelphis albiventris). Histochemistry 88, 37–40 (1987). https://doi.org/10.1007/BF00490164
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DOI: https://doi.org/10.1007/BF00490164