Histochemistry

, Volume 43, Issue 1, pp 73–79 | Cite as

Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei

  • Eberhard Unger
  • Igor B. Buchwalow
  • Manfred Hartmann
  • Wladimir M. Kleschtschinow
  • Nathan T. Raikhlin
Article

Summary

Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C. 2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition by formaldehyde/ethanol prefixation. In isolated mouse liver nuclei activity of NAD-pyrophosphorylase was found in nucleoli, in interchromatin granules, coiled bodies and strand-like structures in nucleoplasm.

Keywords

Public Health Pyrophosphate Mouse Liver Coiled Body ATPase Inhibition 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Eberhard Unger
    • 1
  • Igor B. Buchwalow
    • 2
  • Manfred Hartmann
    • 1
  • Wladimir M. Kleschtschinow
    • 2
  • Nathan T. Raikhlin
    • 2
  1. 1.Central Institute of Microbiology and Experimental TherapyAcademy of SciencesJenaGerman Democratic Republic
  2. 2.Institute of Experimental and Clinical OncologyAcademy of Medical SciencesMoscowUSSR

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