Histochemistry

, Volume 43, Issue 1, pp 59–61 | Cite as

Are all the neuronal nuclei polyploid?

  • Alain Bregnard
  • Alois Knüsel
  • Clive C. Kuenzle
Article

Summary

The DNA analysis of rat brain nuclei by two independent cytochemical methods, namely microfluorometry and UV-absorption, brings completely different results to those published previously by many investigators. The neuronal nuclei possess twice as much DNA as the glial nuclei.

Keywords

Public Health Neuronal Nucleus Brain Nucleus Cytochemical Method Nucleus Polyploid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altman, J.: DNA metabolism and cell proliferation. In: Handbook of neurochemistry, ed. Abel Lajtha, vol. 2, p. 137–182. New York, London: Plenum Press 1969Google Scholar
  2. Burdman, J.A.: Incorporation in vivo of radioactive leucine into neuronal and glial nuclear proteins of rat brain. J. Neurochem. 17, 1555–1562 (1970)Google Scholar
  3. Cohen, J., Mares, V., Lodin, Z.: DNA content of purified preparations of mouse Purkinje neurons isolated by a velocity sedimentation technique. J. Neurochem. 20, 651–657 (1973)Google Scholar
  4. Fujita, S.: DNA cytofluorometry on large and small cell nuclei stained with pararosaniline Feulgen. Histochemie 36, 193–199 (1973)Google Scholar
  5. Knüsel, A., Lehner, B., Kuenzle, C.C., Kistler, G.S.: Isolation of neuronal nuclei from rat brain cortex. J. Cell Biol. 59, 762–765 (1973)Google Scholar
  6. Lentz, R.D., Lapham, L.W.: Postnatal development of tetraploid DNA content in rat Purkinje cells: A quantitative cytochemical study. J. Neuropath. exp. Neurol. 29, 43–56 (1970)Google Scholar
  7. Novakova, V., Sandritter, W., Schlueter, G.: DNA content of neurons in rat central nervous system. Exp. Cell Res. 60, 454–456 (1970)Google Scholar
  8. Ruch, F.: Determination of DNA content by microfluorometry. In: Introduction to quantitative cytochemistry, ed. Wied, G.L., p. 281–294. New York: Acad. Press Inc. 1966Google Scholar
  9. Ruch, F., Trapp, L.: Ein Mikroskopfluorometer mit Kurzzeit-Anregung und Schaltfolgesteuerung. Zeiss Informationen 81, (1973)Google Scholar
  10. Sandritter, W., Novakova, V., Pilny, J., Kiefer, G.: Cytophotometrische Messungen des Nukleinsäure- und Proteingehaltes von Ganglienzellen der Ratte während der postnatalen Entwicklung und im Alter. Z. Zellforsch. 80, 145–152 (1967)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Alain Bregnard
    • 1
  • Alois Knüsel
    • 2
  • Clive C. Kuenzle
    • 2
  1. 1.Department of General BotanySwiss Federal Institute of TechnologyZürichSwitzerland
  2. 2.Department of Pharmacology and Biochemistry, School of Veterinary MedicineUniversity of ZürichZürichSwitzerland

Personalised recommendations