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Histochemistry

, Volume 81, Issue 2, pp 149–151 | Cite as

Calcium in myocardial tissue and isolated mitochondria from a teleost

  • I. L. Leknes
  • T. S. Sætersdal
Article

Summary

A method is presented for isolating cardiac mitochondria from bony-fish. Calcium levels in ventricular whole tissue and isolated mitochondria of Gadus virens L. are determined by atomic absorption flame spectroscopy, and were found to be about 8 and 16 nmolCa/mg prot., respectively. In conclusion, the calcium concentration within the myocardial mitochondria in this species may be nearly three times higher than at the outside, and probably these structures serve as a “calcium sink”. The results are compared with those previously reported for mammals.

Keywords

Public Health Calcium Atomic Absorption Calcium Concentration Calcium Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bhattacharya SK, Williams JC, Palmieri GMA (1979) Determination of calcium and magnesium in cardiac and skeletal muscle by atomic absorption spectroscopy using stoichiometric nitrous oxide-acetylene flame. Anal Lett 12 (B 14):1451–1475Google Scholar
  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254Google Scholar
  3. Dhalla NS, Ziegelhoffer A, Harrow JAC (1977) Regulatory role of membrane systems in heart function. Can J Physiol Pharmacol 55:1211–1234Google Scholar
  4. Harrow JAC, Dhalla NS (1978) Influence of quinidine on ATP-linked calcium binding by heart mitochondria and microsomes. In: Kobayashi T, Sano T, Dhalla NS (eds) Recent advances in studies on cardiac structure and metabolism. Vol 2. University Park Press, Baltimore London Tokyo, pp 457–465Google Scholar
  5. Hayat MA (1972) Basic electron microscopy techniques. Van Nostrand Reinhold, New York LondonGoogle Scholar
  6. Justesen N-P, Sætersdal T, Myklebust R, Engedal H (1978) Calcium in isolated mitochondria from the left ventricular wall. Histochemistry 56:203–211Google Scholar
  7. Leknes IL (1980) Ultrastructure of atrial endocardium and myocardium in three species of Gadidae (Teleostei). Cell Tissue Res 210:1–10Google Scholar
  8. Leknes IL (1984) Morphometric analysis of myocardial cells in a teleost. Anat Anzeig 155:169–172Google Scholar
  9. Lentz RW, Harrison CE Jr, Dewey JD, Barnhorst DA, Danielson GK, Pluth JR (1978) Functional evaluation of cardiac sarcoplasmic reticulum and mitochondria in human pathologic states. J Mol Cell Cardiol 10:3–30Google Scholar
  10. Moore CL (1971) Specific inhibition of mitochondrial Ca2+ transport by ruthenium red. Biochem Biophys Res Commun 42:298–305Google Scholar
  11. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212Google Scholar
  12. Rossi CS, Vasington FD, Carafoli E (1973) The effect of ruthenium red on the uptake and release of Ca2+ by mitochondria. Biochem Biophys Res Commun 50:846–852Google Scholar
  13. Sulakhe PV, Dhalla NJ (1971) Excitation-contraction coupling in heart. VII: Calcium accumulation in subcellular particles in congestive heart failure. J Clin Invest 50:1019–1027Google Scholar
  14. Sætersdal T, Engedal H, Röli J, Myklebust R (1980) Calcium and magnesium levels in isolated mitochondria from human cardiac biopsies. Histochemistry 68:1–8Google Scholar
  15. Sætersdal T, Engedal H, Röli J, Jodalen H, Rotevatn S (1981) Calcium and magnesium levels in isolated cardiac mitochondria from mice injected with isoproterenol. Cell Tissue Res 215:13–19Google Scholar
  16. Sætersdal T, Röli J, Engedal H, Jodalen H, Rotevatn S (1982) Protective effects of verapamil against isoprenaline-induced mobilization of mitochondrial calcium and cellular lipid droplets in the myocardium. Res Exp Med 181:39–47Google Scholar
  17. Thakar JH, Wrogemann K, Blanchaer MC (1973) Effect of ruthenium red on oxidative phosphorylation and the calcium and magnesium content of skeletal muscle mitochondria of normal and Bio 14.6 dystrophic hamsters. Biochim Biophys Acta 314:8–14Google Scholar
  18. Wrogemann K, Nylen EG (1978) Mitochondrial calcium overloading in cardiomyopathic hamsters. J Mol Cell Cardiol 10:185–195Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • I. L. Leknes
    • 1
  • T. S. Sætersdal
    • 1
  1. 1.Cellular Cardiology Research Group, Institute of AnatomyUniversity of BergenBergenNorway

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