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Staining and relative determination of elemental contents by the laser microprobe

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Summary

An improved laser microprobe procedure is developed and applied to the measurement of calcium content in microareas of right and left subepicardial muscles. The staining of canine cardiac muscles by Methylene Blue solution (1% w/v) was found to improve sampling efficiency. Elemental content is proportional to T −1/γ, where T is the transmittance of the characteristic emission line of the element of the photographic plate and γ is its contrast. In the present system, the calcium content is analyzed using T −3.2. We find that the staining of samples and the determination of T −1/γ are useful procedures in the application of laser microprobe to the study of elemental content in biologic microareas.

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Authors and Affiliations

  1. Department of Physiology, Yokohama City University School of Medicine, 232, Yokohama, Japan

    H. Gotoh

  2. Research Fellow of Japan Electron Optics Laboratory, Akishima City, Tokyo, Japan

    M. Murota

  3. Department of Physiology, Teikyo University School of Medicine, Itabashiku, Tokyo, Japan

    A. Kamiyama

Authors
  1. H. Gotoh
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  2. M. Murota
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  3. A. Kamiyama
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Gotoh, H., Murota, M. & Kamiyama, A. Staining and relative determination of elemental contents by the laser microprobe. Histochemistry 63, 323–328 (1979). https://doi.org/10.1007/BF00490060

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  • DOI: https://doi.org/10.1007/BF00490060

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