Skip to main content
SpringerLink
Log in

Immunocytochemical demonstration of the neurosecretory X-organ complex in the eyestalk of the crab Carcinus maenas

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

An antiserum was obtained by immunizing rabbits with sinus gland extracts from Carcinus maenas. The antiserum is almost exclusively directed against neurosecretory material in the medulla terminalis X-organ (MTGXO), as demonstrated by the peroxidase—antiperoxidase (PAP) staining method in light and electron microscopic studies. Radioimmunological binding studies indicate the presence of antibodies against the crustacean hyperglycemic hormone (CHH) or the black pigment dispersing hormone (BPDH) in the antiserum. The results suggest that the neurosecretory perikarya of the MTGXO are the sites of production of CHH and/or BPDH.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adiyodi, K.G., Adiyodi, R.G.: Endocrine control of reproduction in decapod crustacea. Biol. Bull. 45, 121–165 (1970)

    Google Scholar 

  • Andrew, R.D., Orchard I., Saleuddin, A.S.M.: Structural re-evaluation of the neurosecretory system in the crayfish eyestalk. Cell Tissue Res. 190, 235–246 (1978)

    Google Scholar 

  • Andrew, R.D., Shivers, R.R.: Ultrastructure of neurosecretory granule exocytosis by crayfish sinus gland induced with ionic manipulation. J. Morphol. 150, 253–278 (1976)

    Google Scholar 

  • Bliss, D.E., Welsh, J.H.: The neurosecretory system of brachyuran crustacea. Biol. Bull. 103, 157–169 (1952)

    Google Scholar 

  • Carlisle, D.B., Dohrn, P.F.R.: Studies on Lysmata seticaudata Risso (Crustacea, Decapoda). II. Experimental evidence for a growth- and moult-accelerating factor obtainable from eyestalks. Pubbl. Staz. Zool. Napoli 24, 69–83 (1953)

    Google Scholar 

  • Davis, B.J.: Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–422 (1969)

    Google Scholar 

  • Enami, M.: The sources and activities of two chromatophorotropic hormones in crabs of the genus Sesarma. II. Histology of incretory elements. Biol. Bull. 101, 241–258 (1951)

    Google Scholar 

  • Guyselman, J.B.: An analysis of the molting process in the fiddler crab, Uca pugilator. Biol. Bull. 104, 115–137 (1953)

    Google Scholar 

  • Hanström, B.: Neue Untersuchungen über Sinnesorgane und Nervensysteme der Crustaceen II. Zool. Jahrb. (Abt. Anat.) 58, 367–520 (1933)

    Google Scholar 

  • Hunter, W.M., Greenwood, F.C.: Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194, 495–496 (1962)

    Google Scholar 

  • Jaros, P.P.: Tracing of neurosecretory neurons in crayfish optic ganglia by cobalt iontophoresis. Cell Tissue Res. 194, 297–302 (1978)

    Google Scholar 

  • Keller, R.: Comparative electrophoretic studies of crustacean neurosecretory hyperglycemic and melanophore-stimulating hormones from isolated sinus glands. J. Comp. Physiol. 122, 359–373 (1977)

    Google Scholar 

  • Kleinholz, L.M., Keller, R.: Endocrine regulation in crustacea. In: Hormones and evolution. Barrington, E.J.W. (ed.). New York, London: Academic Press 1979

    Google Scholar 

  • Kurup, N.G., Scheer, B.T.: Control of protein synthesis in an anomuran crustacean. Comp. Biochem. Physiol. 18, 971–973 (1966)

    Google Scholar 

  • Lenel, R., Veillet, A.: Effects de l'ablation des pédoncules oculaires sur les pigments caroténoides du crabe Carcinus maenas. C.R. Acad. Sci 233, 1064–1065 (1951)

    Google Scholar 

  • Meusy, J.-J.: Precisions nouvelles sur l'ultrastructure de la glande du sinus d'un crustaceé décapode brachyoure, Carcinus maenas L. Bull. Soc, Zool. Fr. 93, 291–299 (1968)

    Google Scholar 

  • Moriarty, G.C., Halmy, N.S.: Adrenocorticotropin production by the intermediate lobe of the rat pituitary. An electron microscopic-immunohistochemical study. Z. Zellforsch. 132, 1–14 (1972)

    Google Scholar 

  • Nordmann, J.J.: Ultrastructural appearance of neurosecretory granules in the sinus gland of the crab after different fixation procedures. Cell Tissue Res., 186, 557–563 (1977)

    Google Scholar 

  • Passano, L.: The X-organ sinus gland system in crabs. Anat. Rec. 111, 502 (1951a)

    Google Scholar 

  • Passano, L.: The X-organ, a neurosecretory gland controlling molting in crabs. Anat. Rec. 111, 559 (1951b)

    Google Scholar 

  • Rehm, M.: Observation on the localization and chemical constitution of neurosecretory material in nerve terminals in Carcinus maenas. Acta Histochem. (Jena) 7, 88–106 (1959)

    Google Scholar 

  • Smith, G.: The ultrastructure of the sinus gland of Carcinus maenas (Crustacea: Decapoda). Cell Tissue Res. 155, 117–125 (1974)

    Google Scholar 

  • Smith, G., Naylor, E.: The neurosecretory system of the eyestalk of Carcinus maenas (Crustacea: Decapoda). J. Zool. 166, 313–321 (1972)

    Google Scholar 

  • Sternberger, L.A.: Immunocytochemistry. Englewood Cliffs, NJ: Prentice Hall 1974

    Google Scholar 

  • Strolenberg, G.E.C.M., Van Helden, H.P.M., Van Herp, F.: The ultrastructure of the sinus gland of the crayfish Astacus leptodactylus (Nordmann). Cell Tissue Res. 180, 203–210 (1977)

    Google Scholar 

  • Werner, W., Rey, H.G., Wielinger, H.: Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/POD-Methode. Z. Anal. Chem. 252, 224–228 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Zoophysiologie, Universität Bonn, Endenicher Allee 11-13, D-5300, Bonn, Federal Republic of Germany

    P. P. Jaros

Authors
  1. P. P. Jaros
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by the Deutsche Forschungsgemeinschaft (Ke 206/2)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jaros, P.P. Immunocytochemical demonstration of the neurosecretory X-organ complex in the eyestalk of the crab Carcinus maenas . Histochemistry 63, 303–310 (1979). https://doi.org/10.1007/BF00490058

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00490058

Keywords

Search

Navigation