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Histochemistry

, Volume 63, Issue 3, pp 303–310 | Cite as

Immunocytochemical demonstration of the neurosecretory X-organ complex in the eyestalk of the crab Carcinus maenas

  • P. P. Jaros
Article

Summary

An antiserum was obtained by immunizing rabbits with sinus gland extracts from Carcinus maenas. The antiserum is almost exclusively directed against neurosecretory material in the medulla terminalis X-organ (MTGXO), as demonstrated by the peroxidase—antiperoxidase (PAP) staining method in light and electron microscopic studies. Radioimmunological binding studies indicate the presence of antibodies against the crustacean hyperglycemic hormone (CHH) or the black pigment dispersing hormone (BPDH) in the antiserum. The results suggest that the neurosecretory perikarya of the MTGXO are the sites of production of CHH and/or BPDH.

Keywords

Public Health Microscopic Study Electron Microscopic Study Binding Study Staining Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adiyodi, K.G., Adiyodi, R.G.: Endocrine control of reproduction in decapod crustacea. Biol. Bull. 45, 121–165 (1970)Google Scholar
  2. Andrew, R.D., Orchard I., Saleuddin, A.S.M.: Structural re-evaluation of the neurosecretory system in the crayfish eyestalk. Cell Tissue Res. 190, 235–246 (1978)Google Scholar
  3. Andrew, R.D., Shivers, R.R.: Ultrastructure of neurosecretory granule exocytosis by crayfish sinus gland induced with ionic manipulation. J. Morphol. 150, 253–278 (1976)Google Scholar
  4. Bliss, D.E., Welsh, J.H.: The neurosecretory system of brachyuran crustacea. Biol. Bull. 103, 157–169 (1952)Google Scholar
  5. Carlisle, D.B., Dohrn, P.F.R.: Studies on Lysmata seticaudata Risso (Crustacea, Decapoda). II. Experimental evidence for a growth- and moult-accelerating factor obtainable from eyestalks. Pubbl. Staz. Zool. Napoli 24, 69–83 (1953)Google Scholar
  6. Davis, B.J.: Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–422 (1969)Google Scholar
  7. Enami, M.: The sources and activities of two chromatophorotropic hormones in crabs of the genus Sesarma. II. Histology of incretory elements. Biol. Bull. 101, 241–258 (1951)Google Scholar
  8. Guyselman, J.B.: An analysis of the molting process in the fiddler crab, Uca pugilator. Biol. Bull. 104, 115–137 (1953)Google Scholar
  9. Hanström, B.: Neue Untersuchungen über Sinnesorgane und Nervensysteme der Crustaceen II. Zool. Jahrb. (Abt. Anat.) 58, 367–520 (1933)Google Scholar
  10. Hunter, W.M., Greenwood, F.C.: Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature 194, 495–496 (1962)Google Scholar
  11. Jaros, P.P.: Tracing of neurosecretory neurons in crayfish optic ganglia by cobalt iontophoresis. Cell Tissue Res. 194, 297–302 (1978)Google Scholar
  12. Keller, R.: Comparative electrophoretic studies of crustacean neurosecretory hyperglycemic and melanophore-stimulating hormones from isolated sinus glands. J. Comp. Physiol. 122, 359–373 (1977)Google Scholar
  13. Kleinholz, L.M., Keller, R.: Endocrine regulation in crustacea. In: Hormones and evolution. Barrington, E.J.W. (ed.). New York, London: Academic Press 1979Google Scholar
  14. Kurup, N.G., Scheer, B.T.: Control of protein synthesis in an anomuran crustacean. Comp. Biochem. Physiol. 18, 971–973 (1966)Google Scholar
  15. Lenel, R., Veillet, A.: Effects de l'ablation des pédoncules oculaires sur les pigments caroténoides du crabe Carcinus maenas. C.R. Acad. Sci 233, 1064–1065 (1951)Google Scholar
  16. Meusy, J.-J.: Precisions nouvelles sur l'ultrastructure de la glande du sinus d'un crustaceé décapode brachyoure, Carcinus maenas L. Bull. Soc, Zool. Fr. 93, 291–299 (1968)Google Scholar
  17. Moriarty, G.C., Halmy, N.S.: Adrenocorticotropin production by the intermediate lobe of the rat pituitary. An electron microscopic-immunohistochemical study. Z. Zellforsch. 132, 1–14 (1972)Google Scholar
  18. Nordmann, J.J.: Ultrastructural appearance of neurosecretory granules in the sinus gland of the crab after different fixation procedures. Cell Tissue Res., 186, 557–563 (1977)Google Scholar
  19. Passano, L.: The X-organ sinus gland system in crabs. Anat. Rec. 111, 502 (1951a)Google Scholar
  20. Passano, L.: The X-organ, a neurosecretory gland controlling molting in crabs. Anat. Rec. 111, 559 (1951b)Google Scholar
  21. Rehm, M.: Observation on the localization and chemical constitution of neurosecretory material in nerve terminals in Carcinus maenas. Acta Histochem. (Jena) 7, 88–106 (1959)Google Scholar
  22. Smith, G.: The ultrastructure of the sinus gland of Carcinus maenas (Crustacea: Decapoda). Cell Tissue Res. 155, 117–125 (1974)Google Scholar
  23. Smith, G., Naylor, E.: The neurosecretory system of the eyestalk of Carcinus maenas (Crustacea: Decapoda). J. Zool. 166, 313–321 (1972)Google Scholar
  24. Sternberger, L.A.: Immunocytochemistry. Englewood Cliffs, NJ: Prentice Hall 1974Google Scholar
  25. Strolenberg, G.E.C.M., Van Helden, H.P.M., Van Herp, F.: The ultrastructure of the sinus gland of the crayfish Astacus leptodactylus (Nordmann). Cell Tissue Res. 180, 203–210 (1977)Google Scholar
  26. Werner, W., Rey, H.G., Wielinger, H.: Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/POD-Methode. Z. Anal. Chem. 252, 224–228 (1970)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • P. P. Jaros
    • 1
  1. 1.Institut für ZoophysiologieUniversität BonnBonnFederal Republic of Germany

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