, Volume 76, Issue 4, pp 425–438 | Cite as

Fine structural details of human muscle fibres after fibre type specific glycogen depletion

  • M. Sjöström
  • J. Fridén
  • B. Ekblom


Type 1 and Type 2 fibres of skeletal muscle (human m. vastus lateralis), selectively depleted of glycogen by sustained submaximal muscular exercise (running 30 km), were identified at light and electron microscopical level by examination of thin and ultra-thin serial sections treated particularly for visualization of glycogen. Averaged images, obtained by lateral smearing of depleted fibres (Type 1) exhibited five clearly visible cross-bridges in the M-band and had broad Z-bands. Nondepleted fibres (Type 2) showed either three central strong and two weak outer lines in the M-band and intermediate Z-bands (Type 2A), or only three central strong lines in the M-band and narrow Z-bands (Type 2B). The depleted fibres had no subsarcolemmal accumulation of glycogen particles and practically no intermyofibrillar particles. The remaining particles were small in size and seemed almost rudimentary. In nonexercised individuals, a peculiar distribution of individual glycogen particles in the I-band and A-band was found. This distribution was accounted by the structural arrangement of the myofibrillar material.


Fibre Type Microscopical Level Vastus Lateralis Human Muscle Average Image 
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  1. Ashby B, Frieden C, Bishoff R (1979) Immunofluorescent and histochemical localization of AMP deaminase in skeletal muscle. J Cell Biol 81:361–373Google Scholar
  2. Bylund AC, Bjurö T, Cederblad G, Holm J, Lundholm K, Sjöström M, Ängquist KA, Scherstén T (1977) Physical training in man. Eur J Appl Physiol 36:151–169Google Scholar
  3. Brooke MH, Kaiser KK (1970) Muscle fiber types. How many and what kind? Arch Neurol. 23:369–379Google Scholar
  4. Costill DL, Gollnick PD, Jansson ED, Saltin B, Stein EM (1973) Glycogen depletion pattern in human muscle fibres during distance running. Acta Physiol Scand 89:374–383Google Scholar
  5. Dubowitz V, Brooke MH (1973) Muscle biopsy: A modern approach. WB Saunders, London Philadelphia Toronto, pp 5–33Google Scholar
  6. Essén B (1978) Glycogen depletion of different fibre types in human skeletal muscle during intermittent and continous exercise. Acta Physiol Scand 103:446–455Google Scholar
  7. Franzini-Armstrong C (1970) Details of the I band structure as revealed by the localization of ferritin. Tissue Cell 2:327–338Google Scholar
  8. Gollnick PD, Piehl K, Saltin B (1974) Selective glycogen depletion pattern in human muscle fibres after exercise of varying intensity and at varying pedalling rates. J Physiol 241:45–57Google Scholar
  9. Heuser J (1981) Quick-freeze, deep-etch preparation of samples for 3-D electron microscopy. Trends Biochem Sci 6:64–68Google Scholar
  10. Hoppeler H, Lüthi P, Claassen H, Weibel ER, Howald H (1973) The ultrastructure of the normal human skeletal muscle. A morphometric analysis on untrained men, women and well-trained orienteers. Pflügers Arch 344:217–232Google Scholar
  11. Knappeis GG, Carlsen F (1968) The ultrastructure of the M-line in skeletal muscle. J Cell Biol 38:202–211Google Scholar
  12. Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–256Google Scholar
  13. Luther PK, Munro PMG, Squire JN (1981) Three-dimensional structure of the vertebrate muscle A-band. III. M-region structure and myosin filament symmetry. J Mol Biol 151:703–730Google Scholar
  14. Luther PK, Squire JM (1978) Three-dimensional structure of the vertebrate muscle M-region. J Mol Biol 125:313–324Google Scholar
  15. Obinata T, Maruyama K, Sugita H, Kohama K, Ebashi S (1981) Dynamics aspects of structural proteins in vertebrate skeletal muscle. Muscle Nerve 4: 456–488Google Scholar
  16. Pearse AGE (1961) Histochemistry. Theoretical and applied, Appendix 9. Little Brown, Boston, MA, p 832Google Scholar
  17. Pette D (ed.) (1980) Plasticity of muscle. Walter de Gruyter, BerlinGoogle Scholar
  18. Prince FP, Hikida RS, Hagerman FC, Staron RS, Allen WH (1981) A morphometric analysis of human muscle fibers with relation to fiber types and adaptations to exercise. J Neurol Sci 49:165–179Google Scholar
  19. Sjöström M, Kidman S, Henriksson-Larsén K, Ängquist KA (1982) Z- and M-band appearance in different histochemically defined types of human skeletal muscle fibres. J Histochem Cytochem 30:1–11Google Scholar
  20. Sjöström M, Squire JM (1977a) Fine structure of the A-band in cryo-sections. I. The structure of the A-band of human skeletal muscle fibres from ultra-thin cryo-sections negatively stained. J Mol Biol 109:49–68Google Scholar
  21. Sjöström M, Squire JM (1977b) Cryo-ultramicrotomy and myofibrillar fine structure: a review. J Microsc 111:239–278Google Scholar
  22. Somlyo AV, Gonzales-Serratos H, Shuman H, McClellan G, Somlyo (1981) Calcium release and ionic changes in the sarcoplasmic reticulum of tetanized muscle: an electron-probe study. J Cell Biol 90:577–594Google Scholar
  23. Squire J, Edman A-C, Freundlich A, Harford J, Sjöström M (1982) Muscle structure, cryomethods and image analysis. J Microsc 125:215–225Google Scholar
  24. Squire JM, Harford JJ, Edman A-C, Sjöström M (1982) Fine structure of the A-band in cryosections. III. Crossbridge distribution and the axial structure of the human C-zone. J Mol Biol 155:467–494Google Scholar
  25. Strehler EE, Pelloni G, Heizmann CW, Eppenberger HM (1980) Biochemical and ultrastructural aspects of Mr 165,000 M-protein in cross-striated muscle. J Cell Biol 86:775–783Google Scholar
  26. Thiéry JP (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microsc 6:987Google Scholar
  27. Trinick J, Lowey S (1977) M-protein from chicken pectoralis muscle: isolation and characterization. J Mol Biol 131:343–368Google Scholar
  28. Trinick JA (1981) End-filaments: A new structural element of vertebrate skeletal muscle thick filaments. J Mol Biol 151:309–314Google Scholar
  29. Weibel E (ed.) (1979) Stereological methods, Vol 1. Academic Press, New YorkGoogle Scholar
  30. Yarom R, Meiri V (1971) N lines in striated muscle: a site of intracellular Ca2+ Nature (New Biol) 234:254–256Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • M. Sjöström
    • 1
    • 2
  • J. Fridén
    • 1
    • 2
  • B. Ekblom
    • 1
    • 2
  1. 1.Departments of Neurology and AnatomyUniversity of UmeåUmeåSweden
  2. 2.Department of Physiology IIIKarolinska InstitutetStockholmSweden

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