Histochemistry

, Volume 79, Issue 2, pp 227–236 | Cite as

Immunocytochemistry of the microtubules of fat-laden cells

Brown fat cells and adrenocortical cells in primary monolayer culture
  • H. Sugihara
  • N. Yonemitsu
  • K. Ohta
  • S. Miyabara
  • A. Nagayama
Article

Summary

Cytoplasmic microtubules of fat-laden cells containing fine lipid droplets, such as brown fat cells and adrenocortical cells, were studied in relation to the metabolism of intracellular lipid. In these cells, the amount and distribution of lipid droplets reflect the state of inherent cellular function. Materials used were primary monolayer culture of fetal rat brown fat cells and that of bovine adrenocortical cells. The method was the immunocytochemistry with anti-tubulin antibody. When brown fat cells were being lipolyzed or the steroidogenesis of adrenocortical cells were being stimulated, the cytoplasmic microtubules in the cells were organized in a radial pattern in response to the behavior of the lipid droplets. It is assumed that the microtubules were in the regulation of cellular function in terms of the metabolism of lipid droplets in these cells. We have devised, in the course of the current study, a double fluorescence technique as an observational method whereby microtubules were observed immunocytochemically and lipid droplets by a secondary fluorescence with phosphine E staining.

Keywords

Public Health Lipid Cellular Function Phosphine Lipid Droplet 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • H. Sugihara
    • 1
  • N. Yonemitsu
    • 1
  • K. Ohta
    • 1
  • S. Miyabara
    • 1
  • A. Nagayama
    • 2
  1. 1.Department of PathologySaga Medical SchoolSagaJapan
  2. 2.Department of MicrobiologySaga Medical SchoolSagaJapan

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