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Histochemistry

, Volume 79, Issue 2, pp 167–176 | Cite as

Immunocytochemical localization of pepsinogen in rat stomach

  • S. Suzuki
  • C. Furihata
  • S. Tsuyama
  • F. Murata
Article

Summary

The localization of pepsinogen in rat stomachs was investigated by a postembedding immunoferritin method. When the preparations embedded in Epon were used, the secretory granules of chief cells were stained heavily and the granules of mucous neck cells were stained moderately. The secretory granules of cells intermediate between mucous neck cells and chief cells showed a bizonal staining; the electron dense parts were stained heavily and the electron lucent parts were stained moderately. The secretory granules of pyloric gland cells, on the other hand, were labeled faintly. However, the secretory granules of surface mucous cells, foveolar mucous cells, endocrine cells, cardiac mucous cells and cardiac serous cells were not stained by the method. The protein A-gold method showed a similar staining pattern of pepsinogen to that of the immunoferritin method. When the samples embedded in Lowicryl K4M were used to enhance the stainability of pepsinogen, essentially the same staining pattern as that of the samples embedded in Epon was obtained. In addition, the Golgi apparatus and the rough surfaced endoplasmic reticulum were more easily stained.

Keywords

Golgi Apparatus Staining Pattern Secretory Granule Endocrine Cell Gland Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Suzuki
    • 1
  • C. Furihata
    • 2
  • S. Tsuyama
    • 1
  • F. Murata
    • 1
  1. 1.Department of AnatomyKagoshima University School of MedicineKagoshimaJapan
  2. 2.Department of Molecular Oncology, Institute of Medical ScienceUniversity of TokyoTokyoJapan

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