Histochemical localization of adenylate cyclase in cultured sympathetic neurons
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The catecholamine-stimulated adenylate cyclase activity was histochemically demonstrated at ultrastructural level in the cultured neurons of the sympathetic ganglia of the chick embryo.
Adenylate cyclase activity was demonstrated in the axolemma of the axons and axon terminals, however not inside the axons. Heavy precipitates of the enzymatic reaction product was observed intracellularly in some cell processes identified as dendrites of the sympathetic neurons.
In contrast to the extensive reaction in the neuropil, the reaction for the catecholamine-stimulated adenylate cyclase was scarse in the perikarya of the neurons. Only occasional spots of adenylate cyclase activity could be demonstrated in the plasmalemma of some neurons, and no reaction product was observed in the cytoplasm.
No difference in the localization of the adenylate cyclase activity was observed when either dopamine or noradrenaline were used for stimulation.
The possible roles of the catecholamine-stimulated adenylate cyclase in the function of the interneuronal connnections between the aminergic neurons of the sympathetic ganglion are discussed.
KeywordsPublic Health Dopamine Noradrenaline Enzymatic Reaction Adenylate Cyclase
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- Goldstein, M., Ebstein, B., Bronaugh, R.L., Roberge, C.: Stimulation of striatal tyrosine hydroxylase by cyclic AMP. In: Chemical tools in catecholamine research (Almgren, O., Carlsson, A., Engel, J., eds.), Vol. II, pp. 257–264. Amsterdam: North-Holland Publishing Comp. 1975Google Scholar
- Greengard, P.: Presynaptic and postsynaptic roles of cyclic AMP and protein phosphorylation at catecholaminergic synapses. In: Chemical tools in catecholamine research, (Almgren, O., Carlsson, A., Engel, J., eds.), Vol. II, pp. 249–256. Amsterdam: North-Holland Publishing Comp. 1975Google Scholar
- Greengard, P., Kebabian, J.W.: Role of cyclic AMP in synaptic transmission in the mammalian peripheral nervous system. Fed. Proc. 33, 1059–1067 (1974)Google Scholar
- Greengard, P., McAfee, D.A., Kebabian, J.W.: On the mechanism of action of cyclic AMP and its role in synaptic transmission. Advances in cyclic nucleotide research, Vol. 1: Physiology and pharmacology of cyclic AMP. (Greengard, P., Paoletti, R., Robinson, G.A., eds.), New York: Raven Press 1972Google Scholar
- Hervonen, H.: Histochemical and electron microscopical study on sympathetic ganglia of chick embryo in culture. Acta Instit. anat. Univ. Helsinki., Suppl. 8, 1–36 (1975a)Google Scholar
- Hervonen, H.: Differentiation of sympathicoblasts in cultures of chick ganglia. Light and electron microscpic, fluorescence and enzyme histochemical observations. Anat. Embryol. 146, 225–243 (1975b)Google Scholar
- Hervonen, H., Rechardt, L.: Observations on closed tissue cultures of sympathetic ganglia of chick embryos in media buffered with N-Tris-(Hydroxymethyl) Methyl-Glysine or N-2Hydroxy-Ethylpiperazine-N-2-Ethanesulfonic acid. Acta physiol. scand. 90, 267–277 (1974a)Google Scholar
- Hervonen, H., Rechardt, L.: Light and electron microscopic demonstration of cholinesterases of the cultured sympathetic ganglia of the chick embryo. Histochemistry 39, 129–142 (1974b)Google Scholar
- Hungen, K. von, Roberts, S.: Neurotransmitter-sensitive adenylate cyclase systems in the brain. Reviews of neuroscience, Vol. 1 (Ehrenpreis, S., Kopin, I.J., eds.). New York: Raven Press 1974Google Scholar
- Lemay, A., Jarett, L.: Pitfalls in the use of lead nitrate for the histochemical demonstration of adenylate cyclase activity. J. Cell Biol. 65, 39–50 (1975)Google Scholar
- McAfee, D.A., Schorderet, M., Greengard, P.: Adenosine 3′, 5′,-mono-phosphate in nervous tissue: Increase associated with synaptic transmission. Science 171, 1156–1158 (1971)Google Scholar
- Rechardt, L., Härkönen, M.: Electron microscopical demonstration of adenylate cyclase activities in the nervous tissue. Ultrastruct. Res. 50, 365 (1975)Google Scholar
- Rechardt, L., Härkönen, M.: Electron microscopical demonstration of adenyl cyclase activity in nervous tissue (submitted for publication) (1976)Google Scholar
- Wagner, R.C., Bitensky, M.W.: Adenylate cyclase. In: Electron microscopy of enzymes, Vol. 2, Principles and methods (Hayat, M.A., ed.), pp. 110–131. New York: Van Nostrand Reinhold Comp. 1974Google Scholar