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Image analysis of FITC-immunofluorescence histochemistry in perivascular substance P-positive nerves

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Summary

The ability of image analysis to quantify nerves labelled by FITC-immunofluorescence histochemistry has been tested on whole-mount stretch preparations of perivascular substance P-positive nerves. It has been shown that image analysis can make consistent measurements of nerves demonstrated by immunohistochemistry despite the fading of FITC-fluorescence which occurs during storage and on exposure to ultraviolet light.

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Authors and Affiliations

  1. Department of Anatomy and Embryology, and Centre for Neuroscience, University College London, Gower Street, WC1E 6BT, London, England

    T. Cowen

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  1. T. Cowen
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Cowen, T. Image analysis of FITC-immunofluorescence histochemistry in perivascular substance P-positive nerves. Histochemistry 81, 609–610 (1984). https://doi.org/10.1007/BF00489543

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  • DOI: https://doi.org/10.1007/BF00489543

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