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Lipids in the developing enamel of the rat incisor

Parallel histochemical and biochemical investigations

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Summary

Morphologic examination of the developing enamel of rat incisors showed the presence of cell processes and remnants. Histochemical investigation of rapid-frozen freeze-substituted samples, using p-phenylenediamine or a phosphotungstic acid chromic acid mixture, revealed osmiophilic components which were extractable in chloroform-methanol solution and were located inside the tubule-like structures of the extracellular matrix. The presecretory cell-rich and developing enamel zones underwent quantitative and qualitative lipid analysis. Comparison of the biochemical data as well as of the morphological observations, suggests a cellular origin for enamel lipids randomly adsorbed by extracellular matrix components during enamel processing. The nature of the material which appeared as an osmiophilic intra-tubular filling is still unresolved.

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References

  • Ackman RG (1963) Influence of column temperature in the gas-liquid chromatographic separation of methyl esters of fatty acids on polyester substrates. J Gas Chromatogr 1:11–16

    Google Scholar 

  • Beynon AD (1976) A reappraisal of Sudan black staining of enamel matrix in the mouse molar. Arch Oral Biol 21:83–90

    Google Scholar 

  • Brockerhoff H, Hoyle RJ, Wolmark N (1966) Positional distribution of fatty acids in triglycerides of animal depot fats. Biochim Biophys Acta 116:67–72

    Google Scholar 

  • Chen PS, Toribara TY, Warner H (1956) Microdetermination of phosphorus. Anal Chem 28:1756–1758

    Google Scholar 

  • Davis WL, Jones RG, Knight JP, Hagler HK (1982) Cartilage calcification: an ultrastructural, histochemical and analytical X-ray microprobe study of the zone of calcification in the normal avian epiphyseal growth plate. J Histochem Cytochem 30:221–234

    Google Scholar 

  • Dirksen IR (1977) The role of lipids in calcification. In: Snyder F (ed) Lipid metabolism in mammals, Vol 2. Plenum Press, New York, pp 279–292

    Google Scholar 

  • Dirksen TR, Marinetti GV (1970) Lipids of bovine enamel and dentin and human bone. Calcif Tissue Res 6:1–10

    Google Scholar 

  • Escaig J (1982) New instruments which facilitate rapid freezing at 83K and 6K. J Microsc 126:221–229

    Google Scholar 

  • Fincham AG, Burkland GA, Shapiro IM (1972) Lipophilia of enamel matrix. A chemical investigation of the neutral lipids and lipophilic proteins of enamel. Calcif Tissue Res 9:247–259

    Google Scholar 

  • Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    Google Scholar 

  • Harvey DMR (1982) Freeze-substitution. J Microsc 127:209–221

    Google Scholar 

  • Hayat MA (1981) Osmium tetroxide. In: Fixation for electron microscopy. Academic Press, New York, pp 148–182

    Google Scholar 

  • Hess WC, Lee CY, Peckham SC (1956) The lipid content of enamel and dentin. J Dent Res 35:273–275

    Google Scholar 

  • Irving JT (1959) A histological staining method for sites of calcification in teeth and bone. Arch Oral Biol I:89–96

    Google Scholar 

  • Irving JT (1963) Calcification of the organic matrix of enamel. Arch Oral Biol 8:773–774

    Google Scholar 

  • Ledingham JM, Simpson FO (1970) Intensification of osmium staining by p-phenylenediamine: paraffin and Epon embedding; lipid granules in renal medulla. Stain Technol 45:255–260

    Google Scholar 

  • Ledingham JM, Simpson FO (1972) The use of p-phenylenediamine in the block to enhance osmium staining for electron microscopy. Stain Technol 47:239–243

    Google Scholar 

  • Le Lous M, Bazin S (1980) Evolution of triglycerides in rat granulomatous tissue cells. Stimulation of normal adipose tissue development. Int J Tissue React 2:77–83

    Google Scholar 

  • Morgan TE, Huber GL (1967) Loss of lipid during fixation for electron microscopy. J Cell Biol 32:757–760

    Google Scholar 

  • Mollenhauer HH, Morré DJ (1981) Selective staining of dictoysome-like structures (DLS) for spermatocytes of the guinea pig using phosphotungstic acid at low pH. Eur J Cell Biol 25:340–345

    Google Scholar 

  • Odutuga AA, Prout RES (1974) Lipid analysis of human enamel and dentine. Arch Oral Biol 19:729–731

    Google Scholar 

  • Patterson CM, Kruger BJ, Daley TJ (1977) Lipid and protein histochemistry of enamel. Effects of fluoride. Calcif Tissue Res 29:119–125

    Google Scholar 

  • Pearse AG (1968) Histochemistry: theoretical and applied, vol 1. Churchill, London, p 59

    Google Scholar 

  • Prout RES, Odutuga AA (1974a) Lipid composition of dentine and enamel of rats maintained on a diet deficient in essential fatty acids. Arch Oral Biol 19:725–728

    Google Scholar 

  • Prout RES, Odutuga AA (1974b) The effect of lipid composition on enamel and dentine of feeding a corn oil supplement to rat deficient in essential fatty acids. Arch Oral Biol 19:955–958

    Google Scholar 

  • Prout RES, Odutuga AA (1974c) In vivo incorporation of (1-14C)-linoleic acid into the lipids of enamel and dentine of normal and essential fatty acid deficient rats. Arch Oral Biol 19:1167–1170

    Google Scholar 

  • Prout RES, Odutuga AA, Tring FC (1973) Lipid analysis of rat enamel and dentine. Arch Oral Biol 18:373–380

    Google Scholar 

  • Rambourg A (1967) Détection des glycoprotéines en microscopie électronique: coloration de la surface cellulaire et de l'appareil de Golgi par un mélange acide chromique phosphotungstique. CR Acad Sci 265:1426–1428

    Google Scholar 

  • Robinson C, Lowe NR, Wheatherell JA (1977) Changes in amino-acid composition of developing rat incisor enamel. Calcif Tissue Res 23:19–31

    Google Scholar 

  • Roland JC, Lembi CA, Morré DJ (1972) Phosphotungstic acid as a selective electrodense stain for plasma membranes of plant cells. Stain Technol 47:195–200

    Google Scholar 

  • Searcy KL, Bergquist JL (1960) A new color reaction for the quantitation of serum cholesterol. Clin Chim Acta 5:192–199

    Google Scholar 

  • Scheinmann E, Weinreb M, Wolman M (1962) Histochemical study of the ameloblasts and the enamel matrix in rat molars. J Dent Res 41:1293–1303

    Google Scholar 

  • Shapiro IM, Wuthier RE, Irving JT (1966) A study of the phospholipids of bovine dental tissues. I Enamel matrix and dentine. Arch Oral Biol 11:501–512

    Google Scholar 

  • Skipski VP, Barclay M (1969) Thin layer chromatography of phospholipids. In: Methods in enzymology, vol 14. Academic Press, London, pp 566–586

    Google Scholar 

  • Takuma S, Nagai N (1971) Ultrastructure of rat odontoblasts in various stage of their development maturation. Arch Oral Biol 16:993–1011

    Google Scholar 

  • Terracio L, Schwabe KG (1981) Freezing and drying of biological tissues for electron microscopy. J Histochem Cytochem 29:1021–1028

    Google Scholar 

  • Ward BJ, Gloster JA (1976) Lipid losses during processing of cardiac muscle for electron microscopy. J Microsc 108:41–50

    Google Scholar 

  • Warshawsky H (1979) Radioautographic studies on amelogenesis. J Biol Buccale 7:105–126

    Google Scholar 

  • Warshawsky H, Josephsen K (1981) The behaviour of substances labelled with 3H-proline and 3H-fucose in the cellular processes of odontoblasts and ameloblasts. Anat Rec 200:1–10

    Google Scholar 

  • Warshawsky H, Smith CE (1974) Morphological classification of rat incisor ameloblasts. Anat Rec 179:423–446

    Google Scholar 

  • Yunghans WN, Clark JE, Morré DJ, Clegg ED (1978) Nature of the phosphotungstic acid-chromic acid (PACP) stain for plasma membranes of plants and mammalian sperm. Cytobiologie 17:165–172

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Histology, Faculté de Chirurgie Dentaire, (Université Paris V), 1 rue M. Arnoux, F-92120, Montrouge, France

    M. Goldberg

  2. I.N.S.E.R.M. (U 12), Hôpital des Enfants Malades, 149 rue de Sèvres, F-75730, Paris Cédex 15, France

    M. Lelous & M. Boudin

  3. Laboratoire Technologie Appliquée à la Microscopie Electronique, (C.N.R.S.), 105 boulevard Raspail, F-75006, Paris, France

    F. Escaig

Authors
  1. M. Goldberg
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  2. M. Lelous
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  3. F. Escaig
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  4. M. Boudin
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Goldberg, M., Lelous, M., Escaig, F. et al. Lipids in the developing enamel of the rat incisor. Histochemistry 78, 145–156 (1983). https://doi.org/10.1007/BF00489493

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  • DOI: https://doi.org/10.1007/BF00489493

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