, Volume 47, Issue 4, pp 291–302 | Cite as

A preliminary microspectrofluorometric study of NAD(P) reduction in diBenzo(a, e) Fluoranthene-treated single living cells

  • Jean-Marie Salmon
  • Elli Kohen
  • Cahide Kohen
  • P. Viallet
  • F. Zajdela


The fluorescence increase, due to NAD(P) reduction, following microelectrophoretic injection of glucose 6-P (G6P) into EL2 and NCTC 8739 single living cells treated with diBenzo(ae)Fluoranthene (diB(ae)F) and non-treated, has been studied with a rapid microspectrofluorometer. This study shows the enhanced capacity of treated cells to utilize larger doses (6–10 times more) of G6P than control cells. The time course of the return to the initial fluorescence level is essentially related to the magnitude of the injection dose. There are alterations (e.g. red & blue shifts) in the fluorescence spectrum of diB(ae)F-treated cells before injection and in the increase spectrum after injection of G6P, as compared to the same spectra in the diB(ae)F-untreated cells. This is discussed in reference to the metabolization of diB(ae)F as an alternative pathway for the reoxidation of NAD(P)H.


Public Health Glucose Living Cell Control Cell Fluorescence Spectrum 
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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Jean-Marie Salmon
    • 1
  • Elli Kohen
    • 2
    • 3
    • 4
  • Cahide Kohen
    • 2
    • 3
    • 4
  • P. Viallet
    • 1
  • F. Zajdela
    • 5
  1. 1.Laboratoire de Chimie Physique, CentreUniversitaire de PrepignanPerpignanFrance
  2. 2.Papanicolaou Cancer Research InstituteMiamiUSA
  3. 3.Clinical Faculty. Department of PathologySchool of Medicine University of MiamiMiamiUSA
  4. 4.Adjunct Faculty, Department of PhysiologySchool of Medicine University of MiamiMiamiUSA
  5. 5.Unité de Physiologie Cellulaire-U-22 Faculte des Sciences Institut National de la Santé et de la Recherche MedicaleOrsayFrance

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