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Isotachophoretic analysis of organic acids in human seminal plasma

Isotachophoretische Analyse organischer Säuren im menschlichen Samenplasma

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Zusammenfassung

Die vorliegende Arbeit beschäftigt sich mit der isotachophoretischen Analyse von Pyrophosphat, Phosphat und einer Vielzahl organischer Säuren im menschlichen Samenplasma. Alle Proben wurden mittels Ultrafiltration enteiweißt. Phosphat, Citrat und Lactat wurden mit einer Genauigkeit von 3% quantitativ erfaßt. Ferner konnten Oxalat, Pyruvat, Malat, Succinat und Ascorbat in geringen Mengen nachgewiesen werden. Die Isotachophorese eignet sich hervorragend für eine umfassendere Erforschung der Fertilitätsstörungen des Mannes.

Summary

The present study deals with the isotachophoretic analysis of pyrophosphate, phosphate and a variety of organic acids in human seminal plasma. All samples were deproteinized by ultrafiltration. Phosphate, citrate and lactate were quantitatively determined with a precision of 3%. Small amounts of oxalate, pyruvate, malate, succinate and ascorbate were also detected. Isotachophoresis might facilitate future research on the nature of infertility and sterility.

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References

  1. Oefner P, Bonn G, Bartsch G (in press)

  2. Everaerts FM, Beckers JL, Verheggen TP (1976) Isotachophoresis, theory, instrumentation and applications. Elsevier Scientific Publishing Co, Amsterdam

    Google Scholar 

  3. Holloway CJ, Trautschold I (1982) Fresenius Z Anal Chem 311:81–93

    Google Scholar 

  4. Kühnau J, Nowakowski H (1960) Endokrinologie 40:1–12

    Google Scholar 

  5. Nikkanen V (1978) Andrologia 10:464–466

    Google Scholar 

  6. Schirren C, Laudahn G, Heinze I, Hartmann E, Richter E (1979) Andrologia 11:393–401

    Google Scholar 

  7. Mann T, Lutwak-Mann C (1981) Male reproductive function and semen. Themes and trends in physiology, biochemistry and investigative andrology. Springer, Berlin Heidelberg New York

    Google Scholar 

  8. Pérez-Plaza M, Padrón RS, Más J, Peralta H (1982) Intern J Androl 5:6–10

    Google Scholar 

  9. Holmdahl T, Mastroianni L (1965) Fert Steril 16:587–594

    Google Scholar 

  10. Jutte N, Grootegoed JA, Rommerts FFG, van der Molen HJ (1981) J Reprod Fert 62:399–405

    Google Scholar 

  11. Mita M, Hall PF (1982) Biol Reprod 26:445–455

    Google Scholar 

  12. Kaiser KP, Hupf H (1979) Deut Lebensm Rundsch 75:300–304

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Urology, University of Innsbruck, Anichstr 35, A-6020, Innsbruck

    P. J. Oefner & G. Bartsch

  2. Institute of Radiochemistry, University of Innsbruck, Innrain 52A, A-6020, Innsbruck, Austria

    G. Bonn

Authors
  1. P. J. Oefner
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  2. G. Bonn
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  3. G. Bartsch
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Oefner, P.J., Bonn, G. & Bartsch, G. Isotachophoretic analysis of organic acids in human seminal plasma. Z. Anal. Chem. 320, 175–178 (1985). https://doi.org/10.1007/BF00488684

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  • DOI: https://doi.org/10.1007/BF00488684

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