Kinetic assay of monoamine oxidase with 2,2′-azino-di(3-ethylbenzthiazoline-6-sulfonate) as chromogen

  • Ivan D. Iavanović
  • Nada Majkić-Singh
Posters A Enzymatic Analysis


We propose this method as a simple, quick and precise procedure for MAO catalytic activity assay in tissues. By optimization of the reaction, the zero-order kinetics were obtained enabling the initial measurement at 410 nm. We employed ABTS as chromogen; it is not carcinogenic, has good solubility and the oxidation product has an acceptable molar absorption coefficient.


Oxidation Physical Chemistry Analytical Chemistry Inorganic Chemistry Absorption Coefficient 
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Kinetische Methode für die Bestimmung der Monoamin-Oxidase mit 2,2-Azino-di(3-ethylbenzthiazolin-6-sulfonat) als Chromogen


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Ivan D. Iavanović
    • 1
  • Nada Majkić-Singh
    • 2
  1. 1.Central Laboratory of the Military Medical AcademyBelgradeYugoslavia
  2. 2.Department of BiochemistryPharmaceutical FacultyBelgradeYugoslavia

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