Biochemical Genetics

, Volume 12, Issue 2, pp 163–180 | Cite as

RNA-bound reverse transcriptase in Escherichia coli and in vitro synthesis of a complementary DNA

  • Mirko Beljanski
  • Monique Beljanski
Article

Abstract

RNA-bound reverse transcriptase can be easily distinguished from RNA-free reverse transcriptase and DNA-dependent DNA polymerase after fractionation of extracts from Escherichia coli on a DEAE-cellulose column. This enzyme is capable of synthesizing a DNA-like product in the absence of exogenously added template provided that all four deoxyribonucleoside 5′-triphosphates are present in the incubation mixture. Removal of RNA from the enzyme by RNase leads to a considerably decreased polymerizing activity. The activity can be restored under appropriate conditions either by RNA originating from the enzyme or by transforming RNA excreted by showdomycin-resistant E. coli. Enzyme-bound RNA has several characteristics already found for the transforming RNA. DNA synthesized by RNA-bound reverse transcriptase is complementary to the enzyme-bound RNA.

Key words

reverse transcriptase RNA DNA Escherichia coli 

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Copyright information

© Plenum Publishing Corporation 1974

Authors and Affiliations

  • Mirko Beljanski
    • 1
  • Monique Beljanski
    • 1
  1. 1.Biochimie CellulaireInstitut PasteurParisFrance

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