Abstract
Hybrids between a fibroblastic Chinese hamster cell line (CH23) and a mouse lymphoma cell line (P388F36) were produced and isolated by a simple new method without using selective media and avoiding contact with the parental cells. The chromosomal situation in the two hybrid types (PCM and PCS) isolated suggested that growth on glass surface (PCM) or in suspension (PCS) depended on the number of hamster and mouse chromosomes which existed in the hybrids. Chromosomal stability in hybrids grown as monolayers (PCM) was reached at a stage in which two to four mouse chromosomes coexisted with no fewer than 19 hamster chromosomes. In a study of gene linkage utilizing clones of this hybrid population, five out of nine genes regulating the synthesis of different esterases in the mouse cells used were found to be unlinked.
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Ayad, S. R., and Delinassios, J. G. (1973). Fractionation of nucleic acids from mammalian cells in culture by polylysine-kieselguhr chromatography. J. Chromatog. 85162.
Bendich, A., Visozo, A. D., and Harris, R. G. (1967). Intercellular bridges between mammalian cells in culture. Proc. Natl. Acad. Sci. 571029.
Davidson, R. L., and Ephrussi, B. (1965). A selective system for the isolation of hybrids between L cells and normal cells. Nature (Lond.) 2051170.
Fischer, G. A., and Sartorelli, A. C. (1964). Development, maintenance and assay of drug resistance. Meth. Med. Res. 10247.
Fogh, J., Holmgren, N. B., and Ludovici, P. P. (1971). A review of cell culture contaminations. In Vitro 726.
Goldstein, S., and Lin, C. C. (1972). Somatic cell hybrids between cultured fibroblasts from Galapagos tortoise and the golden hamster. Exptl. Cell Res. 73266.
Ham, R. G. (1965). Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. 53288.
Handmaker, S. D. (1971). Cytogenetic analysis of a Chinese hamster-mouse hybrid cell. Nature (Lond.) 233416.
Harris, H., and Watkins, J. F. (1965). Hybrid cells derived from mouse and man: Artificial heterokaryons of mammalian cells from different species. Nature (Lond.) 205640.
Harris, H., Miller, O. J., Klein, G., Worst, P., and Tachibana T. (1969). Suppression of malignancy by cell fusion. Nature (Lond.) 223363.
Hsu, T. C. (1973). Longitudinal differentiation of chromosomes. Ann. Rev. Genet. 7153.
Kao, F. T., and Puck, T. T. (1970). Genetics of somatic mammalian cells: Linkage studies with human-Chinese hamster cell hybrids. Nature (Lond.) 228329.
Kao, F. T., Johnson, R. T., and Puck, T. T. (1969). Complementation analysis on virusfused Chinese hamster cells with nutritional markers. Science 164312.
Klein, G., Bregula, U., Wiener, F., and Harris, H. (1971). The analysis of malignancy by cell fusion. I. Hybrids between tumour cells and L cell derivatives. J. Cell Sci. 8659.
Koprowski, H., and Knowles, B. (1973). Viruses, immune functions and antigenic determinants in heterokaryons and hybrids. In the Proceedings of the Cell fusion and Hybridization Meeting, Winter Park, Academic Press, New York.
Koyama, H., Yatabe, I., and Ouo, T. (1970). Isolation and characterization of hybrids between mouse and Chinese hamster cell lines. Exptl. Cell Res. 62455.
Littlefield, J. W. (1964). Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145709.
Littlefield, J. W. (1966). The role of drug-resistant markers to study the hybridization of mouse fibroblasts. Exptl. Cell Res. 41190.
Marin, G., and Pugliatti-Crippa, L. (1972). Preferential segregation of homospecific groups of chromosomes in heterospecific somatic cell hybrids. Exptl. Cell Res. 70253.
Marshall-Graves, J. A. (1972). Cell cycles and chromosome replication patterns in interspecific somatic hybrids. Exptl. Cell Res. 7381.
Matsuya, Y., Green, H., and Basilico, C. (1968). Properties and uses of human-mouse hybrid cell lines. Nature (Lond.) 2201199.
Morrow, J., Meagher, D., Ruddle, F., and Patterson, M. K. (1973). Interspecific somatic cell hybridization between asparagine-requiring and drug-resistant cell lines. Exptl. Cell Res. 79368.
Nardone, R. M., Todd, J., Gonzales, P., and Gaffney, E. V. (1965). Nucleoside incorporation into strain L cells: Inhibition by pleuropneumonia-like organisms. Science 1491100.
Ornstein, L., and Davies, B. J. (1964). Disc electrophoresis. Ann. N.Y. Acad. Sci. 121321, 404.
Pitts, J. D. (1971). Molecular exchange and growth control in tissue culture. In Growth Control in Cell Culture, Ciba Foundation Symposium, Churchill, London, p. 89.
Pitts, J. D. (1972). Direct interaction between animal cells. In Silvestri, L. G. (ed.), Cell Interactions, Third Lepetit Colloquium, North-Holland, Amsterdam, p. 277.
Pontecorvo, G. (1971). Induction of directional chromosome elimination in somatic cell hybrids. Nature (Lond.) 230367.
Ruddle, F. H. (1970). Utilization of somatic cells for genetic analysis: Possibilities and problems. In Padykula, H. A. (ed.), Symposium of the International Society for Cell Biology, Vol. 9, Academic Press, New York, p. 233.
Ruddle, F. H. (1971). Linkage studied employing mouse-man somatic cell hybrids. Fed. Proc. 30921.
Ruddle, F. H. (1972). Linkage analysis using somatic cell hybrids. Advan. Hum. Genet. 3173.
Ruddle, F. H., and Harrington, L. (1967). Tissue specific esterase isozymes of the mouse. J. Exptl. Zool. 16651.
Ruddle, F. H., and Nichols, E. A. (1971). Starch gel electrophoretic phenotypes of mouse × human somatic cell hybrids and mouse isozyme polymorphisms. In vitro 7120.
Ruddle, F. H., Shows, T. B., and Roderick, T. H. (1969). Esterase genetics in Mus musculus: Expression, linkage and polymorphism of locus Es-2. Genetics 62393.
Scaletta, L. J., Rushforth, N. B., and Ephrussi, B. (1967). Isolation and properties of hybrids between somatic mouse and Chinese hamster cells. Genetics 57107.
Siniscalco, M., Klinger, H. P., Eagle, H., Koprowski, E., Fujimoto, W. Y., and Seegmiller, J. E. (1969). Hybridizatiin of human diploid strains carrying X-linked mutants and its potentials for studies of somatic cell genetics. Proc. Natl. Acad. Sci. 62793.
Sobel, J. S., Albrecht, A. M., Riehm, H., and Biedler, J. L. (1971). Hybridization of actinomycin D- and amethopteriin-resistant Chinese hamster cells in vitro. Cancer Res. 31297.
Stoker, M. G. P. (1967). Transfer of growth inhibition between normal and virus-transformed cells: Autoradiographic studies using marked cells. J. Cell Sci. 2293.
Subak-Sharp, H., Burk, R. R., and Pitts, J. D. (1966). Metabolic co-operation by cell to cell transfer between genetically different mammalian cells in tissue culture. Heredity 21342.
Subak-Sharp, H., Burk, R. R., and Pitts, J. D. (1969). Metabolic co-operation between biochemically marked mammalian cells in tissue culture. J. Cell Sci. 4353.
Terasiman, T., and Tolmach, L. J. (1963). Growth and nucleic acids synthesis in synchronously dividing populations of Hela cells. Exptl. Cell Res. 30344.
Tjio, J. H., and Puck, T. T. (1958). Genetics of somatic cells. II. Chromosomal constitution of cells in tissue culture. J. Exptl. Med. 108259.
Watkins, J. F., and Chen, L. (1969). Immunization of mice against Ehrlich ascites tumour using a hamster/Ehrlich ascites tumour hybrid cell line. Nature (Lond.) 2231018.
Weiss, M. C., and Green, H. (1967). Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Natl. Acad. Sci. 581104.
Westerveld, A., Meerakhan, P., Visser, R. P. L. S., and Bootsma, D. (1971). Loss of human genetic markers in man-Chinese hamster somatic cell hybrids. Nature New Biol. 23420.
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Ayad, S.R., Delinassios, J.G. Esterase gene expression in Chinese hamster and mouse lymphoma hybrids isolated under nonselective pressure. Biochem Genet 12, 147–161 (1974). https://doi.org/10.1007/BF00487822
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DOI: https://doi.org/10.1007/BF00487822