Analysis of phenolic compounds in spruce needle extracts using an UV-VIS-diode array detector
High-performance liquid chromatography (HPLC) coupled with ultraviolet-visible spectroscopy, using a photodiode-array detector, was applied to the investigation of plant extracts for polyphenolic compounds. Simultaneous detection at different wavelengths and measurement of the UV spectrum of each separated compound during the elution allows an easy and rapid identification of the derivatives of benzoic acid, o-coumaric acid, p-hydroxyacetophenone, stilbenes and flavonoide compounds as well as several catechins. However, as some of these compounds have closely related structures, a characterization by their UV-spectra is insufficient. Hydrolysis with hydrochloric acid and enzymes results in the formation of their monomeric non glycosidated compounds as well as in an increase of the peak area assigned to the monomeric compounds. This technique was applied for the phenol analysis of purified spruce needle extracts of picea abies species.
KeywordsHydrochloric Acid Phenolic Compound Plant Extract Benzoic Acid Catechin
Analyse phenolischer Fichtennadelinhaltsstoffe unter Verwendung eines UV-VIS-Dioden-Array-Detektors
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