Abstract
Somatic cell hybridization studies have suggested that the locus for human thymidine kinase is on a No. 17 or No. 18 chromosome. To evaluate further this possibility, fibroblast cell cultures from patients with partial monosomy 18 and trisomy 18 were assayed for thymidine kinase activity; the enzyme levels in these cell extracts were normal. If the enzyme activities reflect a simple gene dose effect, these results suggest that the human thymidine kinase locus is not situated on the No. 18 chromosome.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bollum, F. J., and Potter, V. R. (1958). Incorporation of thymidine into deoxyribonucleic acid by enzymes from rat tissues. J. Biol. Chem. 233 478.
Breitman, T. R. (1963). The feedback inhibition of thymidine kinase. Biochim. Biophys. Acta. 67 153.
Donahue, R. P., Bias, W. B., Renwick, J. H., and McKusick, V. A. (1968). Probable assignment of the Duffy blood group locus to chromosome 1 in man. Proc. Natl. Acad. Sci. 61 949.
Eker, P. (1965). Activities of thymidine kinase and thymidine deoxyribonucleoside phosphatase during growth of cells in tissue culture. J. Biol. Chem. 240 2607.
Eit, S., Piekarski, L. J., and Dubbs, D. R. (1963). Induction of thymidine kinase by vaccination. J. Mol. Biol. 6 22.
Littlefield, J. W. (1965). Studies on thymidine kinase in cultured mouse fibroblasts. Biochim. Biophys. Acta. 95 14.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 265.
Matsuya, Y., Green, H., and Basilico, C. (1968). Properties and uses of human-mouse hybrid cell lines. Nature 220 1199.
Migeon, B. R., and Miller, C. S. (1968). Human-mouse somatic cell hybrids with single human chromosome (group E): Link with thymidine kinase activity. Science 162 1005.
Migeon, B. R., Smith, S. W., and Leddy, C. L. (1969). The nature of thymidine kinase in the human-mouse hybrid cell. Biochem. Genet. 3 583.
Miller, O. J., Tuck, P. R., Khan, P. M., Shin, S., and Siniscalco, M. (1970). Mitotic separation of two human X-linked genes in man-mouse somatic cell hybrids. Proc. Natl. Acad. Sci. 68 116.
Nabholz, M., Miggiano, V., and Bodmer, W. (1970). Genetic analysis with human-mouse somatic cell hybrids. Nature 223 358.
Nance, W. E., and Engel, E. (1967). Autosomal deletion mapping in man. Science 155 692.
Tjio, J. H., and Puck, T. T. (1958). The somatic chromosomes of man. Proc. Natl. Acad. Sci. 44 1229.
Weiss, M. C., and Green, H. (1967). Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Natl. Acad. Sci. 58 1104.
Weissman, S. M., Smellie, R. M. S., and Paul, J. (1960). Studies on the biosynthesis of deoxyribonucleic acid by extracts of mammalian cells. IV. The phosphorylation of thymidine. Biochim. Biophys. Acta 45 101.
Author information
Authors and Affiliations
Additional information
Supported in part by Grant # MR 0504A-69 from the Division of Mental Retardation, Social and Rehabilitation Service, Department of Health, Education and Welfare, and by the Institute of Child Health and Human Development, Grant # DO 4612, Mental Retardation Research Center, UCLA/NPI.
Rights and permissions
About this article
Cite this article
Crandall, B.F., Parkes, R.S. Thymidine kinase activity and human chromosome No. 18. Biochem Genet 5, 451–456 (1971). https://doi.org/10.1007/BF00487134
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00487134