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Genome amplification and gene expression in the ciliate macronucleus

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Abstract

The focus of this review is on the micronucleus and macronucleus in the ciliated protozoa and the organization and function of the DNA molecules within them. We present (1) some of the structural and functional differences which are known, (2) the genetic evidence for macronuclear units, (3) two hypotheses for the organization of the DNA molecules in the macronucleus to explain these units, and (4) experiments designed to discriminate between these hypotheses. We conclude that the size of the genome is not reduced in the macronucleus and that there are 45 copies of the haploid genome present in the macronucleus of normal strains of Tetrahymena pyriformis and 800 copies in the macronucleus of Paramecium aurelia. The ciliate genome is relatively simple in terms of repeated sequences. However, not all copies of the genes present in the macronucleus may be identical since fractions of differing thermal stability appear after renaturation.

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Authors and Affiliations

  1. Departments of Botany and Zoology, University of Michigan, Ann Arbor, Michigan

    Sally Allen

  2. School of Biological Sciences, University of East Anglia, Norwich, England

    Ian Gibson

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  1. Sally Allen
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  2. Ian Gibson
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The work reported in this paper has been supported by a research grant, GM 15879, from the National Institute of General Medical Sciences, U.S. Public Health Service, and by grants from the Science and Medical Research Councils of Great Britain. We are also grateful to the Center for Human Growth and Development, University of Michigan, for a summer fellowship facilitating collaboration.

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Allen, S., Gibson, I. Genome amplification and gene expression in the ciliate macronucleus. Biochem Genet 6, 293–313 (1972). https://doi.org/10.1007/BF00486122

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  • DOI: https://doi.org/10.1007/BF00486122

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