Abstract
Three distinct isoenzyme sets have been demonstrated for adenylate kinase (AK) from human tissues by the technique of starch gel electrophoresis. In adult and fetal muscle, liver, kidney, brain, spleen, lung, and leukocytes the same set was found, though individual bands had different intensities. In adult red cells and in fetal red cells separate and distinctive isoenzyme sets were observed. However, in all tissues, adult and fetal, the phenotype of an individual was recognizable by the presence or absence of a characteristic cathodal band. The apparent anomaly of the same phenotype being expressed in different isoenzyme sets was resolved by the demonstration that in adult and fetal red cells AK complexed with hemoglobin. The implications of this complex are briefly discussed.
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Benesch, R., Benesch, R. E., and Yu, C. (1968). Reciprocal binding of oxygen and diphosphoglycerate by human haemoglobin. Proc. Natl. Acad. Sci. 59 526.
Bockelmann, W., and Ritter, H. (1968). Tissue variability of the phosphotransferases adenylate kinase and pyruvate kinase. Humangenetik 6 373.
Fildes, R. A., and Harris, H. (1966). Genetically determined variation of adenylate kinase in man. Nature 209 261.
Giblett, E. R. (1969). Genetic Markers in Human Blood, Blackwell, Oxford, p. 516.
Harris, H. (1966). Enzyme polymorphisms in man. Proc. Royal Soc. London B 164 298.
Harris, H. (1969). Enzyme and protein polymorphism. Brit. Med. Bull. 25 5.
Haslam, R. J., and Mills, D. C. B. (1967). The adenylate kinase of human plasma, erythrocytes and platelets in relation to the degradation of adenosine diphosphate in plasma. Biochem. J. 103 778.
Kaplan, M. O. (1968). Nature of multiple molecular forms of enzymes. Ann. N.Y. Acad. Sci. 151 382.
Klethi, J., and Mandel, R. (1968). Tissue determined variations of adenylate kinase. Nature 218 467.
Lo, H. H., and Schimmel, R. R. (1969). Interaction of human haemoglobin with adenine nucleotides. J. Biol. Chem. 244 5084.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 265.
McKusick, V. A. (1968). Mendelian Inheritance in Man, ed. 2, Johns Hopkins Press, Baltimore.
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Supported by grants from the Scottish Hospital Endowments Research Trust (HERT 309) and the Muscular Dystrophy Group of Great Britain.
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Brock, D.J.H. Adenylate kinase isoenzyme patterns in tissues of people of different phenotype. Biochem Genet 4, 617–625 (1970). https://doi.org/10.1007/BF00486099
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DOI: https://doi.org/10.1007/BF00486099