Biochemical Genetics

, Volume 12, Issue 6, pp 441–448 | Cite as

Genetic relationship between β-galactosidase and β-fucosidase in the mouse

  • Reza Seyedyazdani
  • Lars-G. Lundin


Evidence for the identity of β-galactosidase and β-fucosidase enzymes in the house mouse was obtained by examination of the enzyme activities in animals from different crosses between C57BL/Kl mice with high galactosidase and fucosidase activities and DBA/2/Kl mice with low activities. There is a strong correlation between the activities of these two enzymes in different tissues of F2 animals. A comparison of the fractionation properties of β-galactosidase and β-fucosidase showed that the two activities had a parallel distribution and identical thermostability. These data suggest that the same enzyme catalyzes the hydrolysis of both substrates.

Key words

mouse enzymes β-fucosidase β-galactosidase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bosmann, H. B., and Hemsworth, B. A. (1971). Intraneural glycosidases. II. Purification and properties of α-fucosidase, β-fucosidase, α-mannosidase and β-xylosidase of rat cerebral cortex. Biochim. Biophys. Acta 242152.Google Scholar
  2. Chytil, F. (1965). Mammalian β-galactosidases. Biochem. Biophys. Res. Commun. 19630.Google Scholar
  3. Dahlqvist, A., Bull, B., and Thomson, D. L. (1965). Rat intestinal 6-bromo-2-naphthyl glycosidase and disaccharidase activities. II. Solubilization and separation of the small-intestinal enzymes. Arch. Biochem. Biophys. 109159.Google Scholar
  4. Esterly, J. R., Standen, A. C., and Pearson, B. (1967). The histochemical demonstration of intestinal β-d-fucosidase with 5-bromo-4-chloroindole-3-yl-β-d-fucopyranoside. J. Histochem. Cytochem. 15470.Google Scholar
  5. Felton, J., Meisler, M., and Paigen, K. (1974). A locus determining β-galactosidase activity in the mouse. J. Biol. Chem. 2493267.Google Scholar
  6. Furth, A. J., and Robinson, D. (1965). Specificity and multiple forms of β-galactosidase in the rat. Biochem. J. 9759.Google Scholar
  7. Heyworth, R., and Walker, P. G. (1962). Almond-emulsin β-d-glucosidase and β-d-galactosidase. Biochem. J. 83331.Google Scholar
  8. Hsia, D. Y. Y., Makler, M., Semenza, G., and Prader, A. (1966). β-Galactosidase activity in human intestinal lactases. Biochim. Biophys. Acta 113390.Google Scholar
  9. Levvy, G. A., and McAllan, A. (1963). β-d-Fucosidase in the limpet, Patella vulgata. Biochem. J. 87209.Google Scholar
  10. Levvy, G. A., McAllan, A., and Hay, A. J. (1962). Inhibition of glycosidases by aldonolactones of corresponding configuration. 3. Inhibitors of β-d-galactosidase. Biochem. J. 82225.Google Scholar
  11. Lundin, L.-G., and Seyedyazdani, R. (1973). Mendelian inheritance of variations in β-galactosidase activities in the house mouse. Biochem. Genet. 10351.Google Scholar
  12. Meisler, M., and Paigen, K. (1972). Coordinated development of β-glucuronidase and β-galactosidase in mouse organs. Science 177894.Google Scholar
  13. Robinson, D., Price, R. G., and Dance, N. (1967). Separation and properties of β-galactosidase, β-glucosidase, β-glucuronidase and N-acetyl-β-glucosaminidase from rat kidney. Biochem. J. 102525.Google Scholar
  14. Seyedyazdani, R., and Lundin, L.-G. (1973). Association between β-galactosidase activities and coat color in mice. J. Hered. 64295.Google Scholar
  15. Wallenfels, K., and Fischer, J. (1960). Untersuchungen über milchzuckerspaltende Enzyme. X. Die Lactase des Kälberdarms. Hoppe-Seyler's Z. Physiol. Chem. 321223.Google Scholar
  16. Wallenfels, K., and Malhotra, O. P. (1960). β-Galactosidase. In Boyer, P. D., Lardy, H., and Myrbäck, K (eds.), The Enzymes, Vol. 4, 2nd ed., Academic Press, New York, p. 409.Google Scholar
  17. Wiederschain, G. Y., and Prokopenkov, A. A. (1971). β-d-Galactosidase and β-d-fucosidase of pig kidney. Arch. Biochem. Biophys. 158539.Google Scholar

Copyright information

© Plenum Publishing Corporation 1974

Authors and Affiliations

  • Reza Seyedyazdani
    • 1
  • Lars-G. Lundin
    • 1
  1. 1.The Institute for Medical GeneticsUppsalaSweden

Personalised recommendations