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Biochemical Genetics

, Volume 13, Issue 1–2, pp 53–64 | Cite as

Nutritional control of xanthine dehydrogenase. II. Effects on xanthine dehydrogenase and aldehyde oxidase of culturing wild-type and mutant Drosophila on different levels of molybdenum

  • E. J. Duke
  • D. R. Rushing
  • E. Glassman
Article

Abstract

Two new mutants, deficient in aldehyde oxidase and xanthine dehydrogenase, have been isolated from a wild-type stock of Drosophila melanogaster and have been provisionally termed lxdc and lxdd, respectively, as both mutants appear to be allelic with lxd (low xanthine dehydrogenase). An analysis has been made of the effects of dietary molybdenum on lxd, lxdc, lxdd, lao (low aldehyde oxidase), mal (maroon-like eye color), and pac (Pacific) wild-type flies. On the lower dietary levels of 10−3M and 10−2M molybdenum, increases in specific activity of both enzymes were observed only in lxd. Furthermore, two- to three-fold increases in specific activity of both enzymes occurred in all strains, except mal, when cultured on 5×10−2M molybdenum. The lxd and lxdc strains failed to survive on this high concentration of the ion. Similar concentrations of molybdenum had no effect in vitro. An extra electrophoretic band of xanthine dehydrogenase was observed on polyacrylamide gel from extracts of wild-type flies cultured on certain levels of molybdenum, but its appearance was not always correlated with the increases in specific activity.

Key words

aldehyde oxidase xanthine dehydrogenase Drosophila melanogaster molybdenum 

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Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • E. J. Duke
    • 1
  • D. R. Rushing
    • 2
  • E. Glassman
    • 2
  1. 1.Department of ZoologyUniversity CollegeBelfield, DublinIreland
  2. 2.Department of Biochemistry and the Genetics CurriculumUniversity of North CarolinaChapel Hill

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